Étude de souches de Stenotrophomonas maltophilia sécrétrices de BLSE : détection de CTX-M et étude de la virulence

Lavigne, Jean‐Philippe; Gaillard, Julien; Bourg, Gisèle; Tichit, C.; Lecaillon, E.; Sotto, Albert

doi:10.1016/j.patbio.2008.07.013

Cited by 10 publications

(4 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Of the 34 IMP- or CTX-resistant isolates, except E. coli and Pantoea isolates, all other isolates were heterotrophic Gram-negative non-fermentative bacilli, indigenous to wide aquatic environmental sources; they are also known as opportunistic pathogens responsible for nosocomial infections (4, 24). In addition to resistance to IMP or CTX, these bacteria have marked resistance to most β-lactams (penicillins and second and third generation cephalosporins).…”

Section: Discussionmentioning

confidence: 99%

“…Indeed, the majority of our isolates ( n =22) belonged to the genus Pseudomonas , which is characterized by its natural resistance to many β-lactams, including third generation cephalosporins, and non-β-lactams (TE, C and NA), and by its susceptibility to ureidopenicillins, carboxypenicillins, aminoglycosides and fluoroquinolones. S. maltophilia ( n =4) produces metallo-β-lactamase L1 resistant to clavulanate and confers resistance to all β-lactams except ATM, and serine-β-lactamase L2, which is clavulanate susceptible and able to degrade penicillins, cephalosporins and ATM (2, 24). …”

Section: Discussionmentioning

confidence: 99%

“…This synergistic image is characteristic of chromosomic β-lactamase L2 for S. maltophilia (24), while it is indicative of ESBL production for E. coli . PCR analysis and nucleotide sequencing identified the ESBL of E. coli isolate as CTX-M-15.…”

Section: Discussionmentioning

confidence: 99%

“…Gram-negative bacteria in the environment, such as Enterobacteriaceae , Vibrionaceae Pseudomonadaceae , Shewanellaceae , Burkholderiaceae , Xanthomonadaceae and Moraxellaceae , were found to be resistant to major classes of antibiotics such as β-lactams and quinolones and their involvement in infectious diseases is a cause of treatment failure (4, 24). This undesirable evolution of bacteria exposes humans to health risks; indeed, aquatic environments can constitute a reservoir or a return path of microorganisms that can reach humans through the food chain (22, 26, 47) or recreational activities (31, 35).…”

mentioning

confidence: 99%

See 3 more Smart Citations

Antibiotic Resistance and Extended-Spectrum β-Lactamases in Isolated Bacteria from Seawater of Algiers Beaches (Algeria)

et al. 2012

View full text Add to dashboard Cite

The aim of the study was to evaluate bacterial antibiotic resistance in seawater from four beaches in Algiers. The most significant resistance rates were observed for amoxicillin and ticarcillin, whereas they were relatively low for ceftazidime, cefotaxime and imipenem. According to sampling sites, the highest resistance rates were recorded for 2 sites subjected to chemical and microbiological inputs (amoxicillin, 43% and 52%; ticarcillin, 19.6% and 47.7%), and for 2 sites relatively preserved from anthropogenic influence, resistance rates were lowest (amoxicillin, 1.5% and 16%; ticarcillin, 0.8% and 2.6%). Thirty-four bacteria resistant to imipenem (n=14) or cefotaxime (n=20) were identified as Pseudomonas aeruginosa (n=15), Pseudomonas fluorescens(7), Stenotrophomonas maltophilia(4), Burkholderia cepacia(2), Bordetella sp. (1), Pantoea sp. (1), Acinetobacter baumannii(1), Chryseomonas luteola(1), Ochrobactrum anthropi(1) and Escherichia coli(1). Screening for extended spectrum β-lactamase showed the presence of CTX-M-15 β-lactamase in the E. coli isolate, and the encoding gene was transferable in association with the IncI1 plasmid of about 50 kbp. Insertion sequence ISEcp1B was located upstream of the CTX-M-15 gene. This work showed a significant level of resistance to antibiotics, mainly among environmental saprophytic bacteria. Transmissible CTX-M-15 was detected in E. coli; this may mean that contamination of the environment by resistant bacteria may cause the spread of resistance genes.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%