Etude de l’efficacité d’une technique de coproscopie quantitative pour le diagnostic de routine et le contrôle des infestations parasitaires des bovins, ovins, équins et porcins

-The ability of the nematophagous fungus Duddingtonia flagrans to reduce the number of infective nematode larvae in coproculture was investigated in goats using different doses of chlamydospores (0, 1.25 × 10 5 , 2.5 × 10 5 , 5 × 10 5 chlamydospores/kg BW/day) given by oral administration or by voluntary consumption in feed during natural or experimental infections with nematodes. The kinetics of excretion of D. flagrans chlamydospores in the faeces was also determined using a dose of 5 × 10 5 chlamydospores/kg BW/day for five days. For all the trials, the faecal nematode egg outputs were determined by a modified McMaster method and standard coprocultures were set up (14 days, 25 °C) to determine the number of larvae emerging from culture in fungus treated and control faeces. When chlamydospores were orally administered, the number of larvae were reduced by 50 to 97% when compared to control cultures. No difference in the level of larval emergence from the culture was seen for experimental or natural infections at the different chlamydospore dose rates. In contrast, when chlamydospores were distributed in the feed, a dosedependent relationship was observed 10 days after the start of administration, the larval development being 2.0%, 14.0% and 86.9% for 5 × 10 5 , 2.5 × 10 5 and 0 spores/kg BW/day, respectively. In addition, the kinetic study showed that the larval emergence from coproculture in the fungus group was statistically lower than in the control group from the second day of administration of the chlamydospores and remained lower until the second day after the last administration (p < 0.05). The results indicate that, for goats in farm conditions, a minimum daily dose of 5 × 10 5 chlamydospores/kg BW must be used to ensure a high treatment efficacy and that daily administration is preferable for maintenance of efficacy over time.goat / biological control / Duddingtonia flagrans / nematode parasite / dose trials

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“…The faecal nematode egg outputs were determined individually using a modified McMaster method with a sensitivity of 15 eggs per gram [20].…”

Section: Measurement Of Fungi Efficiencymentioning

confidence: 99%

Administration of Duddingtonia flagrans chlamydospores to goats to control gastro-intestinal nematodes: dose trials

et al. 2005

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“…At each experimental period, faecal pellets were collected per rectum every two hours from each pair of goats during two days in order to obtain about 1 kg of faeces for each group. Nematode faecal egg output was determined by the McMaster technique [20] on three subsamples of the pooled faecal sample to evaluate the mean faecal contamination for each group at each period (see Tab. I).…”

Section: Faecal Depositions On the Pasturementioning

confidence: 99%

Effect of the nematophagous fungus, Duddingtonia flagrans, on the larval development of goat parasitic nematodes: a plot study

Chartier

Pors

2003

Vet. Res.

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-Effective alternatives to anthelmintic treatment against nematode parasites of goats are required because of the high prevalence of benzimidazole resistance. Towards this objective, the nematophagous fungus, Duddingtonia flagrans (Df), was used in a plot study against two main parasitic nematode species of goats, Teladorsagia circumcincta (Tcir) and Trichostrongylus colubriformis (Tcol). Worm-free, culled goats were experimentally infected with strains of Tcir and Tcol to constitute donors. Half of the animals were periodically given Df chlamydospores at a daily dose of 2.5 × 10 5 spores/kg BW while the remaining animals were kept as controls. At 5 time periods i.e. March, May, July, September and November 2001, corresponding to the main grazing season in France for goats, faeces were collected from the 6th day of fungus administration for the following 2 days to obtain approximately 1 kg of faeces from each group of animals: Tcir/Control, Tcol/Control, Tcir/Fungus, Tcol/Fungus. For each period and each group, the faeces were deposited on a 1 m 2 grass plot and the grass was cut (3 replicates) on weeks 2, 4, 6, 8 ,12 after deposition, for infective larval recovery. Larvae were counted and the results were expressed as a ratio of larvae/ eggs deposited. On the plots with the control faeces deposited in March, July and September, the grass infectivity due to Tcir and Tcol was similar and the maximum number occurred between 2 and 4 weeks post deposition. In May, the maximum numbers of larvae were not recorded until 8 weeks after deposition, due to high daily temperatures and dryness. In November, larval development took place only for Tcir. On the plots with the fungus treated faeces, a significant reduction in grass infectivity occurred for both nematodes and ranged from 50-60% in May, July and November deposits to 80-90% in the September deposit. On the contrary to these findings, no difference was recorded between the fungus and control plots for the March deposit. In conclusion, D. flagrans is suitable for reducing the number of infective larvae in the herbage during the main part of the grazing period for the most important digestive nematodes of goats.goat / biological control / Duddingtonia flagrans / nematode parasite

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“…The intestines were examined with the use of the sedimentation and counting technique (SCT) according to the OIE Manual (20) adapted from the previously described method (10,24). Moreover, samples of faeces (1-5 g) were collected from distal part of the rectum (51 red foxes and 50 raccoon dogs) and examined with the use of McMaster flotation method with Raynaud modification (25).…”

Section: Methodsmentioning

confidence: 99%

Intestinal helminths of raccoon dogs (Nyctereutes procyonoides) and red foxes (Vulpes vulpes) from the Augustów Primeval Forest (north-eastern Poland)

Karamon

Samorek-Pieróg

Moskwa

et al. 2016

Journal of Veterinary Research

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Introduction: The aim of the study was to determine the prevalence of intestinal helminths in red foxes (Vulpes vulpes) and raccoon dogs (Nyctereutes procyonoides) in the Augustów Primeval Forest (north-eastern Poland), with particular regard to zoonotic parasites. Material and Methods: Intestines from 53 raccoon dogs and 66 red foxes were examined with the use of sedimentation and counting technique (SCT). Samples of faeces from 51 red foxes and 50 raccoon dogs were examined with the use of flotation method. Results: Parasitic helminths were found by SCT in 98.5% of red foxes and 96.2% of raccoon dogs. Both species were infected with: Alaria alata (93.9% and 94.3%, respectively), hookworms (68.2% and 83.0%), Apophallus spp. (7.6% and 15.1%), Mesocestoides spp. (57.6% and 24.5%), Taenia spp. (40.9% and 1.9%), and Toxocara/Toxascaris nematodes (33.3% 15.1%). Echinococcus multilocularis was detected only in red foxes (6.1%), but trematodes Echinostomatidae and nematodes Molineus spp. only in raccoon dogs (18.9% and 41.5%, respectively). Additionally, Capillaria spp. eggs were detected by flotation method in 78.4% of foxes and 20.0% of raccoon dogs. Conclusion: The study showed a very high percentage of red foxes and raccoon dogs infected with intestinal helminths in the Augustów Primeval Forest. Moreover, dangerous zoonotic parasites also were found, which should be taken into consideration in the assessment of infection risk for humans in this region.

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Etude de l’efficacité d’une technique de coproscopie quantitative pour le diagnostic de routine et le contrôle des infestations parasitaires des bovins, ovins, équins et porcins

Cited by 217 publications

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Administration of Duddingtonia flagrans chlamydospores to goats to control gastro-intestinal nematodes: dose trials

Administration of Duddingtonia flagrans chlamydospores to goats to control gastro-intestinal nematodes: dose trials

Effect of the nematophagous fungus, Duddingtonia flagrans, on the larval development of goat parasitic nematodes: a plot study

Intestinal helminths of raccoon dogs (Nyctereutes procyonoides) and red foxes (Vulpes vulpes) from the Augustów Primeval Forest (north-eastern Poland)

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