Etude De l'ACTH Plasmatique Au Cours De 100 Epreuves a La Lysine-Vasopressine Chez l'HOMME

Binoux, M; Gourmelen-Combourieu, M.; Luton, J. P.; Pham-Huu-Trung, M T; Girard, F; Courvalin, Jean-Claude; Raux, M C; Lebrun, M. F.; Marrec, D.

doi:10.1530/acta.0.0680001

Cited by 16 publications

(11 citation statements)

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“…However, no dose-dependency could be ob¬ served. This finding is in agreement with results from human studies, showing that LVP-induced pituitary ACTH release can be stimulated to a certain extent only (Rinne & Nordström 1967;Brostoff et al 1968;Binoux et al 1971;Toft et al 1971;Andersson et al 1972). To our knowledge, no data on ADH-induced corticosteroid release in the pig have as yet been published.…”

Section: Discussionsupporting

confidence: 92%

Corticotropin- and lysine-vasopressin induced changes of plasma corticosteroids and testosterone in the adult male pig

Hahmeier

Fenske

Pitzel

et al. 1980

Acta Endocrinologica

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Intravenous injection of 10.0 \g=m\g/kgbody weight synthetic corticotropin (1-24 ACTH) into chronically cannulated boars resulted in significantly elevated plasma corticosteroid and testosterone levels between 20 and 140 min (corticosteroids) and 20\p=n-\80 min (testosterone) after injection. Administration of lysine-vasopressin (LVP) at doses of 0.1, 0.2 and 0.4 IU/kg body weight elicited a significant increase of plasma corticosteroids between 20 and 40 min after injection; on the other hand, plasma testosterone concentrations tended to fall when compared to pre-treatment levels. From our results it can be concluded that exogenously applied ACTH can effectively stimulate the release of corticosteroids and testosterone. Intravenous administration of LVP results in significantly, although not maximally increased plasma corticosteroid concentrations; the release of endogenous ACTH induced by LVP injection, on the other hand, appeared to be too small to stimulate testosterone release significantly.When administering short-acting corticotropin (ACTH) (Liptrap & Raeside 1975;Holtz et al. 1978) or cortisol (Liptrap & Raeside 1978) to pigs, significantly elevated plasma corticosteroid and testosterone levels were obtained. This indicates that both exogenous ACTH and cortisol exert a stimulatory effect upon testosterone secretion. We therefore decided to compare the effects of an elevation of endogenous ACTH on plasma corticosteroid and testosterone levels to that by iv ACTH injection. Endogenous ACTH was increased by injecting lysine-vasopressin which is known to pos¬ sess corticotropin-releasing activity in a number of mammalian species (for ref. see Yates & Maran 1974). Material and MethodsSeventeen adult male miniature pigs (Göttingen strain) with an average body weight of 38 kg (36-40 kg) were used. Thev were kept in individual pens in the same room under natural lighting conditions and fed a stand¬ ard diet containing 15.59? crude protein and 12.59? crude fibre. One week before test performance the animals were fitted with permanently indwelling cathe¬ ters leading to the external jugular vein. The catheter consisted of a silicone rubber tube (Silastic, Dow Corning; length: 40.0 cm, inner diameter: 1.57 mm, outer dia¬ meter: 3.18 mm) which ended inside the cranial vena cava close to the right atrium of the heart. The free end was brought sc to a stab wound in the dorsal neck region.The catheter was secured in the body bv a disc of silicone rubber sheeting (1 mm thick, 30 mm in diameter), which was attached to the rubber with 382 Elasomer adhesive (Dow Corning) close to where it entered the jugular vein.Between blood collections the catheters were filled with sterile saline (0.99? sodium chloride solution) containing 100 IU/ml heparin (Liquemin 25.000®, Hoffmann-La Roche, Grenzach). Blood samples were withdrawn before (referred as basal levels) and 20, 30, 40, 80 and 140 min after iv injection of saline, ACTH or LVP. The heparin¬ ized samples were centrifuged immediately and plasma (5.0 ml) was stored at -20°C un...

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Section: Discussionsupporting

confidence: 92%

Corticotropin- and lysine-vasopressin induced changes of plasma corticosteroids and testosterone in the adult male pig

Hahmeier

Fenske

Pitzel

et al. 1980

Acta Endocrinologica

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“…A constant finding in our superfusion experiments, as well as in most static incubations, was the high stimulatory effect of LVP, which was also found to be highly effective in vivo in NS (Binoux et al 1971).…”

Section: Discussionsupporting

confidence: 74%

In-vitro secretion of ACTH in Nelson's syndrome

Lüdecke¹,

Schabet²,

Westphal³

et al. 1982

Acta neurochir

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Cell suspensions of ACTH cell adenomas of 10 patients with Nelson's syndrome were investigated for in-vitro secretion of ACTH. Two incubation systems, one using incubation beakers and the other a superfusion system, were employed. The cells were tested for their reactivity to lysine-vasopressin (LVP), cortisol, and combinations of both. LVP regularly provoked a rapid significant increase of ACTH secretion. The effect of cortisol was heterogeneous. Paradoxical initial stimulatory effects of cortisol were observed. There was a suppressive effect in some patients, which correlates to low proliferative activity in histological evaluation. In both systems a low secretory activity coincided with high proliferative activity in vivo.

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“…9,10,15). No patient with overt or occult ectopic ACTH syndrome responded to CRH even when the test was repeated.…”

Section: Crh Testmentioning

confidence: 95%

“…The diagnostic value of the LVP test in the differ¬ ential diagnosis of Cushing's disease has been em¬ phasized in many early studies (6)(7)(8)(9)(10)(11). However, definite proof of the diagnosis, such as histological verification of the tumour or cure by surgical re¬ moval, was often lacking, especially in the case of Cushing's disease.…”

Section: Crh Testmentioning

confidence: 99%

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The corticotropin-releasing factor test in the differential diagnosis of Cushing's syndrome: A comparison with the lysine-vasopressin test

Tabarin

Galli

Dezou

et al. 1990

Acta Endocrinologica

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The diagnostic accuracy of the CRH test was compared with that of the LVP test in 28 consecutive patients with ACTH-dependent Cushing's syndrome. A false negative response to CRH was found in 3 of 21 patients with pituitary-dependent Cushing's disease and to LVP in 4. The 7 patients with ectopic ACTH secretion were unresponsive to CRH, whereas 2 did respond to LVP. CRH and high-dose dexamethasone tests combination led to concordant results in 79% of patients. In all cases the etiological diagnosis suggested was correct. LVP and high-dose dexamethasone tests combination led to concordant results in only 71 % of patients and the etiological diagnosis suggested was erroneous in one. Individual tolerance to the CRH test was also clearly better than that to the LVP test. It is concluded that the CRH test, alone or in combination with the high-dose dexamethasone test must be preferentially used to the LVP test in the differential diagnosis of ACTH-dependent Cushing's disease.

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Etude De l'ACTH Plasmatique Au Cours De 100 Epreuves a La Lysine-Vasopressine Chez l'HOMME

Cited by 16 publications

References 0 publications

Corticotropin- and lysine-vasopressin induced changes of plasma corticosteroids and testosterone in the adult male pig

Corticotropin- and lysine-vasopressin induced changes of plasma corticosteroids and testosterone in the adult male pig

In-vitro secretion of ACTH in Nelson's syndrome

The corticotropin-releasing factor test in the differential diagnosis of Cushing's syndrome: A comparison with the lysine-vasopressin test

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