Etiology and prevalence of subclinical mastitis in the Manchega sheep at mid-late lactation

Cruz, Mauro; Serrano, Emma; Montoro, Vidal; Marco, Juan Carlos Gutiérrez; Romeo, Marzia; Baselga, R.; Albizu, Iñaki; Amorena, B.

doi:10.1016/0921-4488(94)90108-2

Cited by 41 publications

(8 citation statements)

References 12 publications

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“…Staphylococci were the most common bacteria detected in this study representing a total percentage of 73.08% of positive cultures, with CNS representing 48.72% from these isolates. Similar findings were reported in previous studies by De la Cruz et al (1994) Bergonier and Berthelot (2003) and who recorded that CNS were the most prevalent pathogens of the mammary gland in sheep. The highest infection rate by CNS may be explained by the fact that CNS is encountered in the environment as an environmental pathogen.…”

Section: Discussionsupporting

confidence: 92%

“…Mastitis is one of the most serious health and economic problems in all dairy sheep flocks all over the major sheep breeding countries Jones, 1990 andGlenn, 1996). Although ovine clinical mastitis is typically gangrenous and causes death, the more important economically is subclinical mastitis due to its high prevalence rate (Marco, 1994) and association with decrease in milk production in addition to growth retardation and high mortality rate among lambs in suckling ewes (Gross et al, 1978;Waston and Buswell, 1984;McCarthy et al, 1988;Fthenakis and Jones, 1990;Mavrogenis et al, 1995;Dario et al, 1996;Peris et al, 1996 andSaratsis et al, 1999).…”

Section: Introductionmentioning

confidence: 99%

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Prevalence and Etiology of Subclinical Mastitis in Dairy Ewes at Fayoum Governorate, Egypt

Moawad

Osman

2005

Assiut Veterinary Medical Journal

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Section: Discussionsupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Prevalence and Etiology of Subclinical Mastitis in Dairy Ewes at Fayoum Governorate, Egypt

Moawad

Osman

2005

Assiut Veterinary Medical Journal

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“…The prevalence of subclinical mastitis in the flock investigated was similar to those reported in the literature regarding lactating ewes from different geographical areas (Watkins et al 1991), especially with reference to countries in the Mediterranean basin (Italy, Greece, France and Spain) (De La Cruz et al 1994; Gonzalez-Rodriguez et al 1995; Albenzio et al 2002; Bergonier et al 2003; Contreras et al 2007; Kiossis et al 2007).…”

Section: Discussionsupporting

confidence: 83%

Use of milk amyloid A in the diagnosis of subclinical mastitis in dairy ewes

Miglio

Moscati

Fruganti

et al. 2013

Journal of Dairy Research

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Subclinical mastitis (SM) is one of the most important diseases affecting dairy ewes worldwide, with negative impact on the animal health, farm income and public health. Animals with SM often remain untreated because the disease may not be revealed. Increase in somatic cell count (SCC) and positive bacteriology for mastitis pathogens in milk samples are indicative of SM but the evidence of only one of these alterations must suggest an uncertain SM (UM). UM is defined when positive bacteriological examination (Latent-SM) or SCC>500 000 cells/ml (non-specific-SM) are detected in milk. Nevertheless, SCC and bacteriological examination are expensive, time consuming and are not yet in use at the farm level in dairy ewes. Recently, a sensitive acute phase protein, amyloid A, displaying multiple isoforms in plasma and different body fluids including mammary secretion (milk amyloid A-MAA), has been investigated as a marker of mastitis in cows and, in a few studies, in sheep. The aim of this trial was to compare the concentration of MAA of single udder-halves in ewes with healthy udder-halves (HU-control group) and naturally occurring subclinical mastitis, both confirmed (SM group) and uncertain (UM groups: Latent-SM and non-specific-SM), for monitoring udder health. The reliability of a specific ELISA kit for the measurement of MAA was also tested. During a 3-month trial period, 153 udder halves were assigned to the experimental groups based on their health status: 25 with SM, 40 with UM (11 with latent-SM and 29 with non-specific-SM) and 88 HU. SCC and bacteriological analysis were performed to establish the control and subclinical mastitis groups. MAA concentrations in milk samples were measured using a specific commercially milk ELISA kit. The data were submitted to statistical analysis. Significant (P<0·05) differences among the groups SM, non-specific-SM and HU were detected with the SM having the highest level and HU the lowest. MAA concentration is affected by the udder health status and is a useful indicator of subclinical mastitis and increased SCC in sheep.

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“…Mastitis prevalence in dairy sheep flocks may be quite high, with subclinical mastitis figures, in Portugal, ranging from 1% to 92.5%, with an average prevalence of 32.2% 1 Other countries refer ewes’ prevalence rates between 0% and 85%. 2 – 16 These numbers justify the importance of implementing prophylactic measures such as hygienic procedures during milking routine, which are undoubtedly crucial to reduce microorganism access to the mammary gland. Furthermore, the drying off treatment with antimicrobials, currently used as prophylactic management of mastitis in cattle, has already proved to be effective in the control of mastitis in small ruminants.…”

Section: Introductionmentioning

confidence: 99%

Local and systemic humoral response to ovine mastitis caused by Staphylococcus epidermidis

Queiroga

2018

SAGE Open Medicine

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Objectives:Mastitis is responsible for a decrease in milk yield and quality. Disease control is vital for producers’ profit and for consumer’s welfare. This study aimed at investigating the immune response to Staphylococcus epidermidis intramammary infection.Methods:A total of 14 S. epidermidis isolates from milk collected from ewes with subclinical mastitis were used. Protein extracts were prepared and analysed by sodium dodecyl sulphate–polyacrylamide gel electrophoresis. Immunoblotting assay was performed for the detection of specific IgG and IgA in blood and milk from S. epidermidis mastitic ewes and from healthy animals.Results:The presence of pathogen-specific IgG was detected in blood of both infected and healthy animals. However, in milk, pathogen-specific IgG was only identified in infected animals, while IgA was found in both groups. Proteins with 59 and 43 kDa were recognized by all immunoglobulins screened in blood and milk provided by both healthy and mastitic ewes. In addition, in milk, IgG and IgA for proteins with 35 kDa were also detected.Conclusion:The results have lead to propose a theory for immunoglobulin dynamics in mammary gland’s defence: blood IgG1, specifically targeting intestinal antigens, is transported to the mammary gland with the main purpose of protecting the newborn, while IgG2 is specific for mammary pathogens and is transported to the mammary gland exclusively during inflammation. This study suggests that only local immunization should trigger IgG-producing cells in the mammary gland as a response to mastitis antigens. Moreover, IgA seems to be of crucial value for the defence of the ewe mammary gland, and stimulation strategies towards an increase in IgA should be addressed for mastitis prevention.

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Etiology and prevalence of subclinical mastitis in the Manchega sheep at mid-late lactation

Cited by 41 publications

References 12 publications

Prevalence and Etiology of Subclinical Mastitis in Dairy Ewes at Fayoum Governorate, Egypt

Prevalence and Etiology of Subclinical Mastitis in Dairy Ewes at Fayoum Governorate, Egypt

Use of milk amyloid A in the diagnosis of subclinical mastitis in dairy ewes

Local and systemic humoral response to ovine mastitis caused by Staphylococcus epidermidis

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