Ethylene Production by Tobacco (Nicotiana tabacum) Callus

Abstract

Tobacco callus cultures grown on defined agar-solidified media produced ethylene in differing amounts, which were related to cultural treatment and age of the callus. There was a close correlation between the rate of etbylene production and growth. In darkness, maximal rates occurred in the third week of growth with ethylene production in the range of 750 ni (calius piece)^^ d^' (fr, wt, = 1,5 g),, and in the light, maximal rates occurred in the first week of growth, 200 nl (callus piece)"' d"' (fr, wt, = 200 mg). Growth was also correlated with ethylene production when the latter was altered by exposure of the callus to inhibitors of ethylene synthesis, L-canaline, benzyl isothiocyanate, and 3,5-diiodo-4hydroxy-beazoic acid. No correlation was found following treatment with AgNOj, a presumptive inhibitor of ethylene action. The inhibition of growth and ethylene production by L-canaline was partially reversed by gassing the cultures with etiiyiene (1 ^1/1), A mercuric perchtorate sink had no significant effect on growth, A possible relationship between ethylene evolution and growth is discussed.Abbreviations: BITC, benzyl isothiocyanate; DIHB, 3,5-diiodc-4-hydroxybenzoic acid; IAA, iiidole-3-acetic acid; GC, gas chromatcgraphy. Materials and MethodsCultural procedures. Calltis of tobacco stem pith wa: obtained from Nicotiana tabacum L, cv, Wisconsin 3S 0031-9317/79/080374-07 $03,00/0 © 1979 Physiologia Plantarum Physiol, Plant, 46, 1979

Arabidopsis thaliana ethylene-responsive element binding protein (AtEBP), an ethylene-inducible, GCC box DNA-binding protein interacts with an ocs element binding protein

“…Whether AtEBP expression in callus is due to elevated ethylene levels or other factors is not clear. There are conflicting reports on ethylene production in callus cultures and the amount of ethylene produced appears to be dependent on the culturing conditions (41). Chitinases have been shown to be inducible by ethylene in yam callus (42), suggesting that ethylene levels are not saturating in this tissue type.…”

Differences in the Rates of Ethylene Production and Growth between the Calluses Derived from Rice (Oryza sativaL.) and Soybean (Glycine max(L.) Merr.)

“…Studies on lavandin callus cultures showed that callus growth was not affected by ethylene production (Mensuali-Sodi et al, 1989), but the regeneration of lavandin callus was associated with a low rate of ethylene production (Panizza et al, 1997). Conversely, a close positive correlation between ethylene production and callus growth was found in tobacco (Huxter et al, 1979) as well as in carob callus cultures (Martins-Loucao and Rodriguez-Barrueco, 1983) . From these results, a clear conclusion has not been drawn on the interaction between ethylene production and the growth in tissue culture.…”

“…In plant tissue and cell cultures, the cells are fairly uniform and undiffe~entiated and it is possible to study the studies also gave different results about the correlation between ethylene production and growth even in the same species (Huxter et al, 1979;Koves and Szab6, 1987). It is also known that dicot plants produce a larger amount of ethylene and are more sensitive to· exogenous ethylene than monocot plants (Tittle et al, 1990;Grossmann and Kwiatkowski, 1993).…”

Ethylene Production by Sunflower Cell Suspensions

“…The influence on ethylene, 1-aminocyclopropanecarboxylic acid (ACC), and N-malonyl-ACC (MACC) Undifferentiated plant cell suspensions are able to produce ethylene depending on the kind of plant from which the cell originated but also on their physiological state (2-4, 15, 17, 21, 24). With respect to its physiological significance, data suggested no functional relationship between growth and ethylene synthesis (10,15,24). Ethylene was assumed to be a by-product of actively dividing cells.…”

“…With respect to its physiological significance, data suggested no functional relationship between growth and ethylene synthesis (10,15,24). Ethylene was assumed to be a by-product of actively dividing cells.…”