<i>Arabidopsis thaliana</i>
            ethylene-responsive element binding protein (AtEBP), an ethylene-inducible, GCC box DNA-binding protein interacts with an ocs element binding protein

Büttner, Michael; Singh, Karam B.

doi:10.1073/pnas.94.11.5961

Cited by 328 publications

(264 citation statements)

References 59 publications

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“…6, K-O). AtERF1b, AtERF2, and AtEBP1 are ET-responsive activators of GCC-box genes (Bü ttner and Singh, 1997;Fujimoto et al, 2000).The weakly up-regulated AtRCD1 modulates ET, abscisic acid (ABA), and methyl-JA responses (Ahlfors et al, 2004). Two uncharacterized putative TFs, At5g07580 and At5g25190, annotated as possibly involved in ET signaling, are down-regulated (Supplemental Table XIII), one yields no significant signal.…”

Section: Ethylenementioning

confidence: 99%

Transcription Analysis of Arabidopsis Membrane Transporters and Hormone Pathways during Developmental and Induced Leaf Senescence

et al. 2006

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A comparative transcriptome analysis for successive stages of Arabidopsis (Arabidopsis thaliana) developmental leaf senescence (NS), darkening-induced senescence of individual leaves attached to the plant (DIS), and senescence in dark-incubated detached leaves (DET) revealed many novel senescence-associated genes with distinct expression profiles. The three senescence processes share a high number of regulated genes, although the overall number of regulated genes during DIS and DET is about 2 times lower than during NS. Consequently, the number of NS-specific genes is much higher than the number of DIS-or DET-specific genes. The expression profiles of transporters (TPs), receptor-like kinases, autophagy genes, and hormone pathways were analyzed in detail. The Arabidopsis TPs and other integral membrane proteins were systematically reclassified based on the Transporter Classification system. Coordinate activation or inactivation of several genes is observed in some TP families in all three or only in individual senescence types, indicating differences in the genetic programs for remobilization of catabolites. Characteristic senescence type-specific differences were also apparent in the expression profiles of (putative) signaling kinases. For eight hormones, the expression of biosynthesis, metabolism, signaling, and (partially) response genes was investigated. In most pathways, novel senescence-associated genes were identified. The expression profiles of hormone homeostasis and signaling genes reveal additional players in the senescence regulatory network.

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Section: Ethylenementioning

confidence: 99%

Transcription Analysis of Arabidopsis Membrane Transporters and Hormone Pathways during Developmental and Induced Leaf Senescence

et al. 2006

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“…Recently, it has been shown that DELLAs counteract the effect of ethylene during apical hook development by inhibiting the activity of ETHYLENE INSENSITIVE3 (EIN3) through physical interaction, providing a mechanism for the coregulation of this process by GAs and ethylene . It was previously shown that RAP2.3 expression is induced by ethylene (Büttner and Singh, 1997). Significantly, EIN3 binds in vivo to the RAP2.3 promoter (Chang et al, 2013) and activates its expression in etiolated seedlings (Fig.…”

Section: Dellas Prevent the Binding Of Rap23 To The Promoter Of Its mentioning

confidence: 78%

“…RAP2.3 belongs to the group VII ERFs of the AP2/EREBP superfamily (Nakano et al, 2006). Given that (1) no member of this family has been previously reported as a DELLA interactor (Locascio et al, 2013b) and (2) it participates in the ethylene signaling cascade (Büttner and Singh, 1997), which is known to control apical hook formation (Guzmán and Ecker, 1990), we decided to investigate the functional connection between GAI and RAP2.3.…”

Section: Identified Della Interactors Affect Ga Responsesmentioning

confidence: 99%

“…In other cases, the interaction with the TF occurs in the vicinity of the promoters of certain genes to promote their expression (Lim et al, 2013;Park et al, 2013). RAP2.3 binds in vitro and in vivo to the GCC box (Büttner and Singh, 1997;Yang et al, 2009;Gibbs et al, 2014), and it is able to activate transcription when transiently overexpressed in Arabidopsis leaves (Yang et al, 2009). To test the effect that the interaction with GAI may have on the transcriptional activation ability of RAP2.3, we performed transactivation assays in leaves of Nicotiana benthamiana.…”

Section: Gai Counteracts Rap23-mediated Transcriptional Activationmentioning

confidence: 99%

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Large-Scale Identification of Gibberellin-Related Transcription Factors Defines Group VII ETHYLENE RESPONSE FACTORS as Functional DELLA Partners

et al. 2014

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DELLA proteins are the master negative regulators in gibberellin (GA) signaling acting in the nucleus as transcriptional regulators. The current view of DELLA action indicates that their activity relies on the physical interaction with transcription factors (TFs). Therefore, the identification of TFs through which DELLAs regulate GA responses is key to understanding these responses from a mechanistic point of view. Here, we have determined the TF interactome of the Arabidopsis (Arabidopsis thaliana) DELLA protein GIBBERELLIN INSENSITIVE and screened a collection of conditional TF overexpressors in search of those that alter GA sensitivity. As a result, we have found RELATED TO APETALA2.3, an ethylene-induced TF belonging to the group VII ETHYLENE RESPONSE FACTOR of the APETALA2/ethylene responsive element binding protein superfamily, as a DELLA interactor with physiological relevance in the context of apical hook development. The combination of transactivation assays and chromatin immunoprecipitation indicates that the interaction with GIBBERELLIN INSENSITIVE impairs the activity of RELATED TO APETALA2.3 on the target promoters. This mechanism represents a unique node in the cross regulation between the GA and ethylene signaling pathways controlling differential growth during apical hook development.

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“…22 In D. japonica callus culture, exogenous ethylene elevated the chitinase activity. 23 In addition, jasmonic acid (JA) acts as signal that promotes defense system in plant and regulates the plant's responses to wounding. The expression of the SafchiA from C. sativus was affected by JA and wounding directly, but not by ethylene, abscissic acid or salicylic acid (SA).…”

Section: Discussionmentioning

confidence: 99%

Purification, characterization of a CkChn134 protein from Cynanchum komarovii seeds and synergistic effect with CkTLP against Verticillium dahliae

et al. 2012

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Cynanchum komarovii Al Iljinski is a desert plant that has been used as analgesic, anthelminthic, and antidiarrheal, but also as herbal medicine to treat cholecystitis in people. In this work, an antifungal protein with sequence homology to chitinase was isolated from C. komarovii seeds and named CkChn134. The three-dimensional structure prediction of CkChn134 indicated that the protein has a loop domain formed a thin cleft, which is able to bind molecules and substrates. The protein and CkTLP synergistically inhibited the fungal growth of Verticillium dahliae, Fusarium oxysporum, Rhizoctonia solani, Botrytis cinerea, and Valsa mali in vitro. The fulllength cDNA was cloned by RT-PCR and RACE-PCR according to the partial protein sequences obtained by nanoESI-MS/MS. The real-time PCR showed that the transcription level of CkChn134 had a significant increase under the stress of ethylene, NaCl, low temperature, drought, and pathogen infection, which indicates that CkChn134 may play an important role in response to abiotic and biotic stresses. The CkChn134 protein was located in the extracellular space/cell wall by CkChn134::GFP fusion protein in transgenic Arabidopsis. Furthermore, overexpression of CkChn134 significantly enhanced the resistance of transgenic Arabidopsis against V. dahliae. Interestingly, the coexpression of CkChn134 and CkTLP showed substantially greater protection against the fungal pathogen V. dahliae than either transgene alone. The results suggest that the CkChn134 is a good candidate protein or gene, and it had a potential synergistic effect with CkTLP for contributing to the development of disease-resistant crops.

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Arabidopsis thaliana ethylene-responsive element binding protein (AtEBP), an ethylene-inducible, GCC box DNA-binding protein interacts with an ocs element binding protein

Abstract: Ocs elements are a group of promoter sequences required for the expression of both pathogen genes in infected plants and plant defense genes. Genes for ocs element binding factors (OBFs), belonging to a specific class of basic-region leucine zipper (

Cited by 328 publications

References 59 publications

Transcription Analysis of Arabidopsis Membrane Transporters and Hormone Pathways during Developmental and Induced Leaf Senescence

Transcription Analysis of Arabidopsis Membrane Transporters and Hormone Pathways during Developmental and Induced Leaf Senescence

Large-Scale Identification of Gibberellin-Related Transcription Factors Defines Group VII ETHYLENE RESPONSE FACTORS as Functional DELLA Partners

Purification, characterization of a CkChn134 protein from Cynanchum komarovii seeds and synergistic effect with CkTLP against Verticillium dahliae

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