Ethylene-Induced Vinblastine Accumulation Is Related to Activated Expression of Downstream TIA Pathway Genes inCatharanthus roseus

Abstract

We selected different concentrations of ethephon, to stress C. roseus. We used qRT-PCR and HPLC followed by PCA to obtain comprehensive profiling of the vinblastine biosynthesis in response to ethephon. Based on our findings, the results showed that the high concentration of ethephon had a positive effect at both transcriptional and metabolite level. Meanwhile, there was a remarkable decrease of hydrogen peroxide content and a promoted peroxidase activity in leaves. The loading plot combination with correlation analysis suggested that CrPrx1 could be regarded as a positive regulator and interacts with ethylene response factor (ERF) to play a key role in vinblastine content and peroxidase (POD) activity. This study provides the foundation for a better understanding of the regulation and accumulation of vinblastine in response to ethephon.

Metabolomics analysis reveals both plant variety and choice of hormone treatment modulate vinca alkaloid production in Catharanthus roseus

“…Similarly to observations in previous studies (Aerts et al., 1994; El‐Sayed & Verpoorte, 2004; Jaleel, Gopi, Gomathinayagam, & Panneerselvam, 2009; Pan et al., 2010; Wang et al, 2016; Zhang et al., 2018), treatment of both LBE and SSA seedlings with either methyl jasmonate or ethephon induced the production of various precursor alkaloids. These hormones also affected the expression levels of several key biosynthetic enzymes and transcription factors.…”

“…Over the last 50 years, laboratory studies of vinca alkaloid production has been induced in planta with MeJA via root uptake from growth medium or through exposure to vapor in an enclosed system (Aerts, Gisi, Carolis, De Luca, & Baumann, 1994; El‐Sayed & Verpoorte, 2004; Rijhwani & Shanks, 1998). Ethylene and its derivative ethephon (EPTN) have more recently been identified as an induction agent for the TIA pathways (Pan et al., 2010; Wang et al, 2016). Foliar application of ETPN, a compound that is quickly converted into ethylene upon uptake into the cell, does not require any special equipment and is a method that can be straightforwardly transferred from a laboratory setting into a greenhouse setting for agricultural‐scale production of these desirable compounds.…”

CrERF5, an AP2/ERF Transcription Factor, Positively Regulates the Biosynthesis of Bisindole Alkaloids and Their Precursors in Catharanthus roseus

“…Significant accumulation of vinblastine was found in response to high concentration of ethylene and Cu, in which ERF and MPK formed a positive feedback loop connecting two pathways actively involved in C. roseus (Pan et al, 2014). Comprehensive profiling of the vinblastine biosynthesis in response to ethephon suggested that ERF could regulate and interact with CrPRX1 to play a key role in vinblastine accumulation and peroxidase activity (Wang X. et al, 2016). These previous researches supported our results that CrERF5, responding to both ethylene and JA signals, might be involved in the bisindole alkaloids accumulation.…”

“…Negative AP2/ERF TF CR1 only involved in the accumulation of vindoline and serpentine in C. roseus (Liu et al, 2017). It was reported that an AP2/ERF TF might interact with CrPRX1 to play a key role in the vinblastine content (Wang X. et al, 2016). But no further investigation was performed.…”

Terpene Moiety Enhancement by Overexpression of Geranyl(geranyl) Diphosphate Synthase and Geraniol Synthase Elevates Monomeric and Dimeric Monoterpene Indole Alkaloids in Transgenic Catharanthus roseus

“…It has been previously reported that the expression of PRX1 and accumulation of 3′,4′-anhydrovinblastine is under complex regulation and also depends on the availability of hydrogen peroxide ( Costa et al, 2008 ). In addition, PRX1 has been suggested to act as positive regulator and interacts with ethylene responsive factor (ERF) regulating accumulation of 3′,4′-anhydrovinblastine in C. roseus ( Wang et al, 2016 ).…”