Metabolomics analysis reveals both plant variety and choice of hormone treatment modulate vinca alkaloid production in <i>Catharanthus roseus</i>

Fraser, Valerie N.; Philmus, Benjamin; Megraw, Molly

doi:10.1002/pld3.267

Cited by 6 publications

(3 citation statements)

References 40 publications

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“…It is used in many plants to induce the accumulation of alkaloids, such as D. Officinale , C. roseus , and T. wilfordii . [ 39 , 40 , 41 ]. However, there have been no reports on the induction of diterpene alkaloids by MJ.…”

Section: Discussionmentioning

confidence: 99%

Integrating Metabolomics and Transcriptomics to Unveil Atisine Biosynthesis in Aconitum gymnandrum Maxim

Chen

Tian

Jin

et al. 2022

IJMS

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Diterpene alkaloids (DAs) are characteristic compounds in Aconitum, which are classified into four skeletal types: C18, C19, C20, and bisditerpenoid alkaloids. C20-DAs are thought to be the precursor of the other types. Their biosynthetic pathway, however, is largely unclear. Herein, we combine metabolomics and transcriptomics to unveil the methyl jasmonate (MJ) inducible biosynthesis of DAs in the sterile seedling of A. gymnandrum, the only species in the Subgenus Gymnaconitum (Stapf) Rapaics. Target metabolomics based on root and aerial portions identified 51 C19-DAs and 15 C20-DAs, with 40 inducible compounds. The highest content of C20-DA atisine was selected for further network analysis. PacBio Isoform sequencing integrated with RNA sequencing not only provided the full-length transcriptome but also their response to induction, revealing 1994 genes that exhibited up-regulated expression. Further, 38 genes involved in terpenoid biosynthesis were identified, including 7 diterpene synthases. In addition to the expected function of the four diterpene synthases, AgCPS5 was identified to be a new ent-8,13-CPP synthase in Aconitum and could also combine with AgKSL1 to form the C20-DAs precursor ent-atiserene. Combined with multiple network analyses, six CYP450 and seven 2-ODD genes predicted to be involved in the biosynthesis of atisine were also identified. This study not only sheds light on diterpene synthase evolution in Aconitum but also provides a rich dataset of full-length transcriptomes, systemic metabolomes, and gene expression profiles, setting the groundwork for further investigation of the C20-DAs biosynthesis pathway.

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Section: Discussionmentioning

confidence: 99%

Integrating Metabolomics and Transcriptomics to Unveil Atisine Biosynthesis in Aconitum gymnandrum Maxim

Chen

Tian

Jin

et al. 2022

IJMS

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“…According to Verrananda (2016), in his research entitled "Identification of Secondary Metabolites and Antioxidant Activity of Tapak Dara Flower Extract (Catharanthus roseus)" stated that the phytochemical screening results contained alkaloids [9][10] [11], flavonoids, phenolics, tannins and terpenoids [12] [13]. And the secondary metabolite group with IC50 value in the tapak dara flower (Catharanthus roseus) shows the natural antioxidant activity as an antidote to free radicals [14].…”

Section: Introductionmentioning

confidence: 99%

Toxicity Test of Catharanthus roseus Flower Extract with Brine Shrimp Lethality Test Method

Sari

Surbakti

Khairani

et al. 2022

IJSE

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Tapak dara (Catharanthus roseus) is a plant belonging to the Apocynaceae family originating from Central America and is generally grown as an ornamental plant. The purpose of this study was to determine the percentage of mortality data and the LC50 value of tapak dara methanol extract (Catharanthus roseus) against Artemia salina Leach larvae using the Brine Shrimp Lethality Test (BSLT) method. The BSLT method used consisted of 6 concentration treatments, namely 20 ppm, 60 ppm, 100 ppm, 140 ppm, 180 ppm, and 0 ppm a negative control, each of which was repeated three times. At each concentration, 10 experimental animals of 48 hours old Artemia salina Leach larvae were used. The toxic effect of the extract was identified by the percentage of mortality of Artemia salina Leach larvae using probit analysis (LC50). The results showed the linear regression equation y = 3.0809x – 12.065. This shows that the mortality of the test animals reached 50% when the compound concentration reached 34,599 ppm. Based on the toxicity test of the methanol extract of the tapak dara flower (Catharanthus roseus) using the Brine Shrimp Lethality Test (BSLT) method, in this study it was toxic because LC50>1000 ppm so it has potential as an anticancer.

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“…Catharanthus roseus (C. roseus), a member of the Apocynaceae, is an ornamental plant in botanical gardens [23]. This plant produces various secondary metabolites during its growth [24] and is a medicinal plant model for secondary metabolism studies [25]. Terpenoid indole alkaloids (TIAs) are the essential secondary metabolites of the species [26].…”

Section: Introductionmentioning

confidence: 99%

A source-sink model explains the difference in the metabolic mechanism of mechanical damage to young and senescing leaves in Catharanthus roseus

Chen

Guo

et al. 2021

BMC Plant Biol

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Background Mechanical damage is an unavoidable threat to the growth and survival of plants. Although a wound to senescing (lower) leaves improves plant vitality, a wound to younger (upper) leaves often causes damage to or death of the whole plant. Source-sink models are often used to explain how plants respond to biotic or abiotic stresses. In this study, a source-sink model was used to explain the difference in the metabolic mechanism of mechanical damage to young and senescing leaves of Catharanthus roseus. Results In our study, GC-MS and LC-QTOF-MS metabolomics techniques were used to explore the differences in source-sink allocation and metabolic regulation in different organs of Catharanthus roseus after mechanical damage to the upper/lower leaves (WUL/WLL). Compared with that of the control group, the energy supplies of the WUL and WLL groups were increased and delivered to the secondary metabolic pathway through the TCA cycle. The two treatment groups adopted different secondary metabolic response strategies. The WLL group increased the input to the defense response after damage by increasing the accumulation of phenolics. A source-sink model was applied to the defensive responses to local (damaged leaves) and systemic (whole plant) damage. In the WUL group, the number of sinks increased due to damage to young leaves, and the tolerance response was emphasized. Conclusion The accumulation of primary and secondary metabolites was significantly different between the two mechanical damage treatments. Catharanthus roseus uses different trade-offs between tolerance (repair) and defense to respond to mechanical damage. Repairing damage and chemical defenses are thought to be more energetically expensive than growth development, confirming the trade-offs and allocation of resources seen in this source-sink model.

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Metabolomics analysis reveals both plant variety and choice of hormone treatment modulate vinca alkaloid production in Catharanthus roseus

Cited by 6 publications

References 40 publications

Integrating Metabolomics and Transcriptomics to Unveil Atisine Biosynthesis in Aconitum gymnandrum Maxim

Integrating Metabolomics and Transcriptomics to Unveil Atisine Biosynthesis in Aconitum gymnandrum Maxim

Toxicity Test of Catharanthus roseus Flower Extract with Brine Shrimp Lethality Test Method

A source-sink model explains the difference in the metabolic mechanism of mechanical damage to young and senescing leaves in Catharanthus roseus

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