Ethyl-methane-sulphonate effects on anther cultures of Nicotiana tabacum

Medrano, H.; Millo, Eduardo Primo; Guerri, José

doi:10.1007/bf00028553

Cited by 15 publications

(2 citation statements)

References 15 publications

(8 reference statements)

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“…Chemical and physical mutagens are available for mutagenic treatment of crop plants. Nevertheless, several chemical mutagens have been applied of which ethyl methane sulfonate (EMS), Nnitroso-N-methylurea (NMU), N-Nitroso, N-Ethylurea (ENU) and sodium azide are the preferred agents in plant mutation induction (Medrano et al, 1986;Szarejko and Forster, 2007). Alkylating agents are the most important chemical mutagens used in mutation breeding.…”

Section: Introductionmentioning

confidence: 99%

Seed viability, germination and seedling growth of canola (Brassica napus L.) as influenced by chemical mutagens

Emrani¹,

Arzani²,

Saeidi³

2011

Afr. J. Biotechnol.

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Mutation induction is considered as an effective way to enrich plant genetic variation, particularly for traits with a very low level of genetic variation. The objectives of this study were to evaluate the effect of different dosages of chemical mutagens on seed germination, seed viability and seedling growth characteristics and to identify optimum treatment conditions for chemical mutagens based on the LD 50 criterion in canola (Brassica napus L.). Two pretreatment conditions of soaking in distilled water and non-soaking, different concentrations of chemical mutagens, and four treatment periods were investigated. The effect of mutagen dosage on seed viability was also assessed using the tetrazolium staining test. Results revealed the significant effects of mutagen dosages and treatment periods on seed viability and seed germination as well as on seedling characteristics for all the mutagens tested. Additionally, it was found that increased dosage and period in each treatment led to significant reductions in seed viability for the tested mutagens. Pretreatment did not significantly influence most of the studied characteristics. The 0.8% ethyl methanesulfonate (EMS) for 6 h, 12 mM N-nitroso-Nethylurea (ENU) and 6 mM sodium azide for 8 h and 9 mM N-nitroso-N-methylurea (NMU) for 4 h were considered as optimum treatment conditions.

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Section: Introductionmentioning

confidence: 99%

Seed viability, germination and seedling growth of canola (Brassica napus L.) as influenced by chemical mutagens

Emrani¹,

Arzani²,

Saeidi³

2011

Afr. J. Biotechnol.

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“…Procedures for producing haploid plants have been established for different plant species (Sunderland 1974, Maheshwari et al 1982. To obtain a useful mutant, anther culture combined with radiation and chemical treatment has been used (Mondeil 1974, Medrano et al 1986, Kinoshita et al 1989, Hasegawa et al 1995.…”

Section: Introductionmentioning

confidence: 99%

Potato Virus Y-resistant Mutation Induced by the Combination Treatment of Ion beam Exposure and Anther Culture in Nicotiana tabacum L.

Hamada

Inoue

Tanaka

et al. 1999

Plant Biotechnology

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To develop an efficient procedure for obtaining a desired mutation, we performed ion beam exposure on tobacco anthers and selected Potato Virus Y (PVY)-resistant mutants in the haploid generation.Anthers were exposed to C ion beams of 5 to 200Gy or He ion beams of 5 to 400Gy, and cultured on modified Nakata's medium. Anther culture response, the percentage of anthers producing plantlets, was markedly reduced by the exposure and, at higher doses, all plantlets were vitrified. RD50 based on the anther culture response was about 5Gy for C ion beams and about 10Gy for He ion beams. The frequency of mitotic cells with chromosome aberrations in root tips of plantlets derived from exposed anthers, ranged from 11.9% to 16.4%, compared to 3.1% in the control.All plants in the non-exposed regimen died within 21 days after inoculation of PVY. On the other hand, 15 of 472 plants in the exposed regimen were viable and continued to grow, with minor disease symptoms observed in some plants after 50 days. Accordingly, these plants were considered to be PVYresistant. In particular, one plant showed no disease symptoms and grew vigorously.

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In Vitro Mutagenesis and Genetic Improvement

Ullah

Naeem

et al. 2011

Technological Innovations in Major World Oil Crops, Volume 2

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Ethyl-methane-sulphonate effects on anther cultures of Nicotiana tabacum

Cited by 15 publications

References 15 publications

Seed viability, germination and seedling growth of canola (Brassica napus L.) as influenced by chemical mutagens

Seed viability, germination and seedling growth of canola (Brassica napus L.) as influenced by chemical mutagens

Potato Virus Y-resistant Mutation Induced by the Combination Treatment of Ion beam Exposure and Anther Culture in Nicotiana tabacum L.

In Vitro Mutagenesis and Genetic Improvement

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