Ethyl Iso-allocholate from a Medicinal Rice Karungkavuni Inhibits Dihydropteroate Synthase in Escherichia coli: A Molecular Docking and Dynamics Study

Kullappan, Malathi; Anbarasu,; Ramaiah, Sudha

doi:10.4172/pharmaceutical-sciences.1000184

Cited by 26 publications

(16 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To identify the chemical constituents in the two crude extracts of F. hamiltonii, the GC-MS technique was used in the study. The five (5) compounds were identified in the dichloromethane extract (FHAD), whereas four (4) compounds were identified in methanol extract (FHAM) of aerial part of F. hamiltonii (Tables 3 and 4) [17][18][19][20][21][22][23][24].…”

Section: Resultsmentioning

confidence: 99%

Biological potential and GC-MS analysis of phytochemicals of Farsetia hamiltonii (Royle)

Hayat¹,

Uzair²

2019

biomedicalresearch

View full text Add to dashboard Cite

www.biomedres.info Farsetia hamiltonii (Royle) is a desert plant and is endemic to the desert area of Pakistan and India. It is an annual shrubby, often woody at base, 10-40 cm long, vary variable in size, densely appressed white hairs, braches erect or sub spreading, leaves oblong linear, thick woody roots, fruit a little longer, narrowed at either end, are distinctive. Flowers are white or pink in color, petals slightly longer than sepals and flowering season is March to September [4]. The medicinal use of plant is to treat pain, inflammation and swelling in joints, diabetes, gastrointestinal and infectious diseases. This plant is used with "Ghee" as a cooling medicine and tonic. Its boiled juice is applied to treat camel wound in Cholistan [3]. The local name is "Fareed Booti" [5]. This plant is selected for study keeping in view the medicinal importance of genus Farsetia. The ethyl alcohol extract of aerial parts of F. aegyptia was in vitro investigated for cytotoxicity and exhibited cytotoxicity against A549 and HepG2 carcinomas. The phenolic rich fraction was further fractionized and isolated a new flavonoid kampferol-8-CO -β-diglucopyranoside which showed the high cytotoxicity against Hela and MCF-7 cell lines [6]. The alcoholic extract of F. aegyptia is evaluated for anti-bacterial and anti-fungal activities which show maximum inhibition against Klebsiella pneumonia and no activity against Candida albicans [7]. According to literature survey, no phytochemical and biological activities on F. hamiltonii have been reported and this species is found unexplored. Methods Enzymes, chemicals and equipments Alpha glucosidase (EC 3.2.1.20), chymotrypsin (EC 3.4.21.1), urease (EC 3.5.1.5), Butyryl cholinesterase (EC 3.1.1.8), Acarbose, Chymostatin, Thiourea, Eserine, chloroform, acetone, diethyl ether, dichloromethane and methanol were purchased from Sigma-Aldrich and Merck companies. Ciprofloxacin, KH2PO4, DTNB [5,5-dithiobis (2-nitrobenzoic acid)], phosphate buffer, N-succinyl phenylalanine-P-nitroanilide and 4-nitrophenyl acetate were of analytical grade. The gram-positive bacteria (Bacillus subtilis, Staphylococcus aureus) and gram-negative (Escherichia coli, Pseudomonas aeruginosa, Salmonella typhi) were used in this study. The instruments synergy HT BioTek 96 microplate reader, Rotary evaporator (Buchi), Vacuum pump, Spectrophotometer (Shimadzo, Japan), EZ-Fit Enzyme Kinetics software (Perrella Scientific Inc. Amherst, USA) and Gas chromatography-mass spectrometry (GC Agilent system, USA) were used.

show abstract

Section: Resultsmentioning

confidence: 99%

Biological potential and GC-MS analysis of phytochemicals of Farsetia hamiltonii (Royle)

Hayat¹,

Uzair²

2019

biomedicalresearch

View full text Add to dashboard Cite

show abstract

“…Although, there is scanty documentation on its biological activity, [40] observed its anti-tumour activity when "monoconjugated to paclitaxel (PTX)." Additionally, Ethyl iso-allocholate also detected in the sweetened drink showed antimicrobial activity [41,42]. The biological function of n-Hexadecanoic acid, 9,12-Octadecadienoic acid (Z,Z)-and oleic acid also identified in the sample was discussed above.…”

Section: Gc-ms Analysis For Sweetenedmentioning

confidence: 86%

GC-MS Characterization of Unsweetened and Sweetened “Kunun-zaki” Beverage and Pulp

Efiong

Nwaedozie

Ubhenin

et al. 2020

AFSJ

View full text Add to dashboard Cite

Aims: To characterize unsweetened and sweetened “kunun-zaki” drink/pulp using GC-MS. Study Design: The experimental groups were divided into four: unsweetened “kunun-zaki” drink, sweetened “kunun-zaki” drink, unsweetened “kunun-zaki” pulp and sweetened “kunun-zaki” pulp respectively. Place of Study: Department of Biochemistry, Federal University of Lafia, Nasarawa State and Central Diagnostic and Research Laboratory, Ilorin, Nigeria. Methodology: The processes involved sorting, cleaning, soaking, decanting, washing, milling, gelatinization, fermentation, and sieving. Unsweetened “kunun-zaki” beverage was made from millet, sorghum, soybean, dried sweet potatoes, germinated rice, ginger, and cloves. The same process was followed for the sweetened variety, with addition of dried dates. After sieving to collect “kunun-zaki” beverage, the remnant was the pulp. Hydrodistillation and Soxhlet apparatus were used to extract the essential oils, and fatty acids respectively. Fatty acid methyl esters of the samples were then analysed using GC-MS. Results: The GC chromatogram identified three constituents in unsweetened “kunun-zaki” drink. The dominant constituents were 9,12-octadecadienenoic acid (z,)- (73.36%), and n-hexadecanoic acid (19.31%). Eleven compounds were identified in sweetened “kunun-zaki” drink. The most abundant was 9, 12-octadecadienoic acid (Z,Z)- 54.062%, followed by n-hexadecanoic acid (17.278%). Twenty-one peaks corresponding to eighteen compounds were detected in unsweetened “kunun-zaki” pulp. The dominant constituents were gingerol (20.262%), 1H-3a,7-methanoazulene,2,3,4,7,8,8a-hexahydro-3,6,8,8-teramethyl-,[3R-(3α,3aβ,7 β,8aα)]- (19.925%), 2-Butanone,4-(4-hydroxy-3-methoxyphenyl)- (15.59%), and cedrene (10.977%). For sweetened “kunun-zaki” pulp, four compounds were detected. The dominant compounds being 9,12-octadecadienoic acid (z,z)- (68.664%), n-hexadecanoic acid (18.654%), and oleic acid (10.199%). Conclusion: The results showed safe fatty acid profile of all samples for different health status. Other components showed bioactivities with significant health benefits. This study identified compounds which validate the medicinal claims of “kunun-zaki.” This is the first documentation of GC-MS profiling of “kunun-zaki” parts.

show abstract

“…The antioxidant activity of phenolic compounds was explained due to their scavenging capacity against the free radicals by donation of hydrogen atoms and electrons as well as chelation of the metal cations [ 19 ]. In addition, polyphenols were reported to have bactericidal and bacteriostatic action via inhibition of the bacterial enzymes and toxins and alter their cytoplasmic membrane as well as they can exert synergistic effect with the commonly used antibiotics and increase their effect on the bacterial pathogens [ [20] , [21] , [22] , [23] ]…”

Section: Discussionmentioning

confidence: 99%

Effect of habitat variations on the chemical composition, antioxidant, and antimicrobial activities of Achillea fragrantissima (Forssk) Sch. Bip

Elsharkawy

Elmorsy

2021

Biotechnology Reports

View full text Add to dashboard Cite

Highlights Achilea fragmentisma plant is widely distributed along northern regions of Saudi Arabia. plant was collected from Tabuk and Arar regions. Geographical variation on the chemical compositions, antimicrobial and antioxidant of the plant. The antioxidant and antimicrobial of the plant showed significant variation in two habitats. Plant collected from Arar showed high antioxidant activity by DPPH radical scavenging methods, and good antibacterial activity with gram-positive bacteria. GC/MS data showed marked variation in chemical compositions of both phenolic and alkaloid in plants collected from both regions. Phenolic were accumulated with higher amounts in Tabouk than Arar region plants.

show abstract

Ethyl Iso-allocholate from a Medicinal Rice Karungkavuni Inhibits Dihydropteroate Synthase in Escherichia coli: A Molecular Docking and Dynamics Study

Cited by 26 publications

References 25 publications

Biological potential and GC-MS analysis of phytochemicals of Farsetia hamiltonii (Royle)

Biological potential and GC-MS analysis of phytochemicals of Farsetia hamiltonii (Royle)

GC-MS Characterization of Unsweetened and Sweetened “Kunun-zaki” Beverage and Pulp

Effect of habitat variations on the chemical composition, antioxidant, and antimicrobial activities of Achillea fragrantissima (Forssk) Sch. Bip

Contact Info

Product

Resources

About