Ethyl glucuronide: Unusual distribution between head hair and pubic hair

Kintz, Pascal; Villain, Marion; Vallet, Emilie; Etter, Mathieu; Salquèbre, Guillaume; Cirimele, Vincent

doi:10.1016/j.forsciint.2007.08.012

Cited by 77 publications

(52 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Even though the GC-MS and GC-MS/MS procedures proved to be accurate and highly sensitive [13], [14], [15], [16], [17] and [18], most toxicology laboratories currently prefer LC-MS/MS methods, because they are faster and no derivatization is needed. Among these, different sample preparation steps, hair amounts and chromatographic conditions were adopted [19], [20], [21], [22], [23], [24], [25], [26], [27] and [28].…”

Section: Introductionmentioning

confidence: 99%

Determination of ethyl glucuronide levels in hair for the assessment of alcohol abstinence

Pirro¹,

Corcia²,

Seganti³

et al. 2013

Forensic Science International

View full text Add to dashboard Cite

This study examined the potential of a highly sensitive LC-MS/MS method for the determination of EtG in head hair (i) to ascertain alcohol abstinence, (ii) to estimate the basal level of EtG (sub-ppb concentrations) in head hair in a population of alcohol abstainers and (iii) to suggest a revision of cut-off values for assessing alcohol abstinence. An UHPLC-MS/MS protocol previously developed was modified and validated again to detect low EtG levels in head hair samples from a population of 44 certain abstainers and teetotalers. Basal level of EtG in hair was determined by a standard addition quantification method. The validated UHPLC-MS/MS method allowed detecting and quantifying 0.5 and 1.0 pg/mg of EtG in hair, respectively. EtG concentrations lower than 1.0 pg/mg were determined for 95% of abstainers; 30% of them had non-detectable (<0.5 pg/mg) EtG values.Two samples evidenced EtG concentrations higher than 1.0 pg/mg that were subsequently explained by unintentional ethanol exposure. The method's feature of high analytical sensitivity makes it particularly suitable for alcohol abstinence ascertainment and, in the same time, allows to tentatively estimate basal EtG concentrations in hair around 0.8 ± 0.4 pg/mg. This finding opens a discussion on the possible origin of basal EtG concentration and potential sources of bias in the evaluation of alcohol abstinence. Cut-off value in the range of 1.0-2.0 pg/mg can be reliably proposed to support alcohol abstinence.

show abstract

Section: Introductionmentioning

confidence: 99%

Determination of ethyl glucuronide levels in hair for the assessment of alcohol abstinence

Pirro¹,

Corcia²,

Seganti³

et al. 2013

Forensic Science International

View full text Add to dashboard Cite

show abstract

“…In the literature there are many papers reporting different cut-off levels to indicate abstinence, moderate and alcohol abuse [11][12][13][14][15][16]. However, there is also a consensus that positive result above cut-off should not be regarded as definitive, above certain levels it would imply that the subject has a probable alcohol abuse.…”

Section: Introductionmentioning

confidence: 99%

Testing for alcohol use in hair: is ethyl glucuronide (EtG) stable in hair?

Tsanaclis¹,

Kingston²,

Wicks³

2009

Ann Toxicol Anal

View full text Add to dashboard Cite

-Purpose: Ethyl glucuronide (EtG) is a biological marker in hair used to indicate abstinence or excessive alcohol consumption. The main aim of this paper is to evaluate the stability of EtG in hair samples as it can potentially be affected by normal hygiene, and affect interpretation of the results. Methods: EtG was measured by GC-MS/MS in 102 hair samples, which were sectioned in three monthly sections when available to produce 291 sections of hair (sectioned set) and 468 hair samples (not sectioned set), where the most recent centimetre was analysed. Results: The 95% percentiles of the EtG levels detected in the not sectioned set and in the first section of the sectioned set were 0.23 ng/mg (N = 468) and 0.22 ng/mg (N = 102), respectively. The 95% percentiles of the levels detected in the second sections and in the third sections were 0.15 ng/mg (N = 102) and 0.10 ng/mg (N = 87), respectively. Levels were below cut-off (0.01 ng/mg) in 61% in the not sectioned set and 67% in the sectioned set. Of the samples where EtG was detected, the second section samples showed mean EtG levels 74% lower the levels detected in the first section. The mean levels detected of EtG in third section were 62% the levels of the second section and 47% the levels detected in the first section. Analysis of variance showed the levels of the third section significantly lower (p < 0.05) than the first section. Conclusion: The results of this study suggest that normal hair hygiene might wash out EtG from the hair. The recommendation is therefore that only the most recent month must reliably monitor abstinence or chronic alcohol abuse using head hair and that data should be evaluated in conjunction with other biochemical tests and clinical evaluation.

show abstract

“…Les poils pubiens semblent plus sensibles que les cheveux de la tête pour mettre en évidence une consommation d'étha-nol [5]. En effet, la gamme des concentrations d'éthyl glucuronide varie de 5 à 5000 pg/mg pour les poils pubiens versus 4 à 300 pour les cheveux.…”

Section: Discussionunclassified

Interprétation des concentrations d’éthyl glucuronide dans les cheveux

Kintz¹

2010

Ann Toxicol Anal

Self Cite

View full text Add to dashboard Cite

Ethyl glucuronide: Unusual distribution between head hair and pubic hair

Cited by 77 publications

References 8 publications

Determination of ethyl glucuronide levels in hair for the assessment of alcohol abstinence

Determination of ethyl glucuronide levels in hair for the assessment of alcohol abstinence

Testing for alcohol use in hair: is ethyl glucuronide (EtG) stable in hair?

Interprétation des concentrations d’éthyl glucuronide dans les cheveux

Contact Info

Product

Resources

About