Ethyl esters (biodiesel) production by Pseudomonas fluorescens lipase immobilized on chitosan with magnetic properties in a bioreactor assisted by electromagnetic field

Cubides-Román, Diana C.; Pérez, Víctor Haber; Castro, Heizir F. de; Orrego, Carlos E.; Giraldo, Óscar; Silveira, Eurípedes Garcia; David, Geraldo Ferreira

doi:10.1016/j.fuel.2017.02.014

Cited by 59 publications

(19 citation statements)

References 37 publications

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“…Microbial lipases are produced by several microbial species, including bacteria and fungi, such as Pseudomonas fluorescens 63 , Burkholderia cepacia 64 , Staphylococcus haemolyticus 65 , Chromobacterium viscosum 66 , Phichia pastoris 67 , Mucor miehei 68 , Aspergillus terreus 69 , Rhizopus oryzae 70 , Candida cylindracea 71 , and Candida rugosa 72 . The potentiality for bacterial lipase production is attributed to their ability to utilize inducers of lipids and fats such as vegetable oil, oil industry waste, cooking oil waste, surfactants, and triglycerides through hydrolytic processes by the inducible lipases 73 .…”

Section: Resultsmentioning

confidence: 99%

Optimizing the catalytic activities of methanol and thermotolerant Kocuria flava lipases for biodiesel production from cooking oil wastes

Najjar

Hassan

Zabermawi

et al. 2021

Sci Rep

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In this study, two highly thermotolerant and methanol-tolerant lipase-producing bacteria were isolated from cooking oil and they exhibited a high number of catalytic lipase activities recording 18.65 ± 0.68 U/mL and 13.14 ± 0.03 U/mL, respectively. Bacterial isolates were identified according to phenotypic and genotypic 16S rRNA characterization as Kocuria flava ASU5 (MT919305) and Bacillus circulans ASU11 (MT919306). Lipases produced from Kocuria flava ASU5 showed the highest methanol tolerance, recording 98.4% relative activity as well as exhibited high thermostability and alkaline stability. Under the optimum conditions obtained from 3D plots of response surface methodology design, the Kocuria flava ASU5 biocatalyst exhibited an 83.08% yield of biodiesel at optimized reaction variables of, 60 ○C, pH value 8 and 1:2 oil/alcohol molar ratios in the reaction mixture. As well as, the obtained results showed the interactions of temperature/methanol were significant effects, whereas this was not noted in the case of temperature/pH and pH/methanol interactions. The obtained amount of biodiesel from cooking oil was 83.08%, which was analyzed by a GC/Ms profile. The produced biodiesel was confirmed by Fourier-transform infrared spectroscopy (FTIR) approaches showing an absorption band at 1743 cm−1, which is recognized for its absorption in the carbonyl group (C=O) which is characteristic of ester absorption. The energy content generated from biodiesel synthesized was estimated as 12,628.5 kJ/mol. Consequently, Kocuria flava MT919305 may provide promising thermostable, methanol-tolerant lipases, which may improve the economic feasibility and biotechnology of enzyme biocatalysis in the synthesis of value-added green chemicals.

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Section: Resultsmentioning

confidence: 99%

Optimizing the catalytic activities of methanol and thermotolerant Kocuria flava lipases for biodiesel production from cooking oil wastes

Najjar

Hassan

Zabermawi

et al. 2021

Sci Rep

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“…Several types of beads have been described in literature using alginate and chitosan [16] , [17] . Many studies have used alginate-chitosan beads to immobilize a wide range of purified enzymes [18] , [19] . The present study demonstrates different entrapment procedures dramatically alter enzymatic loading.…”

Section: Introductionmentioning

confidence: 99%

Enzymatic hydrolysis of starch into sugars is influenced by microgel assembly

Souza

Orsi

Gomes

et al. 2019

Biotechnology Reports

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“…99–125 lipase 30 h 96.0 Li et al 2010 [39] Micro-aqueous phase Sapium sebiferum oil Lipase from Pseudomonas cepacia G63 12 h 97.1 Present work Activated carbon Sardine oil Lipase from Aspergillus niger 10 h 94.0 Yucel, 2011 [40] Olive pomace Pomace oil Lipase from Thermomyces lanuginosus 24 h 93.0 Kawakami et al, 2011 [41] Silica monolith Jatropha oil Burkholderia cepacia lipase 12 h 90.0 Huang et al, 2015 [42] Crystalline PVA microspheres Microalgae Oil Rhizomucor miehei lipase 24 h 90.0 Yan et al 2010 [43] Cross-linking and lipase coating with K 2 SO 4 Waste cooking oil Geotrichum sp. Lipase 4 h 85.0 Kalantari et al 2013 [44] Magnetic silica nanocomposite Soybean oil lipase from Pseudomonas cepacia 24 h 55.0 Roman et al 2017 [45] Chitosan Coconut oil ...…”

Section: Resultsmentioning

confidence: 99%

Production of biodiesel by enzymatic transesterification of waste sardine oil and evaluation of its engine performance

Arumugam

Venkatachalam

2017

Heliyon

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Waste sardine oil, a byproduct of fish industry, was employed as a low cost feedstock for biodiesel production. It has relatively high free fatty acid (FFA) content (32 mg KOH/g of oil). Lipase enzyme immobilized on activated carbon was used as the catalyst for the transesterification reaction. Process variables viz. reaction temperature, water content and oil to methanol molar ratio were optimized. Optimum methanol to oil molar ratio, water content and temperature were found to be 9:1, 10 v/v% and 30 °C respectively. Reusability of immobilized lipase was studied and it was found after 5 cycles of reuse there was about 13% drop in FAME yield. Engine performance of the produced biodiesel was studied in a Variable Compression Engine and the results confirm that waste sardine oil is a potential alternate and low-cost feedstock for biodiesel production.

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Ethyl esters (biodiesel) production by Pseudomonas fluorescens lipase immobilized on chitosan with magnetic properties in a bioreactor assisted by electromagnetic field

Cited by 59 publications

References 37 publications

Optimizing the catalytic activities of methanol and thermotolerant Kocuria flava lipases for biodiesel production from cooking oil wastes

Optimizing the catalytic activities of methanol and thermotolerant Kocuria flava lipases for biodiesel production from cooking oil wastes

Enzymatic hydrolysis of starch into sugars is influenced by microgel assembly

Production of biodiesel by enzymatic transesterification of waste sardine oil and evaluation of its engine performance

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