Enzymatic hydrolysis of starch into sugars is influenced by microgel assembly

Souza, Igor Albuquerque de; Orsi, Daniela Castilho; Gomes, Anderson J.; Lunardi, Claure N.

doi:10.1016/j.btre.2019.e00342

Cited by 13 publications

(4 citation statements)

References 31 publications

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“…Residual sugar contents in the fermentation broth were assessed using the 3,5‐dinitrosalicylic acid (DNS) method (de Souza & Orsi, 2019). The different concentrations of glucose standard solution (0–1 mg/ml and 0.2 mg/ml gradient) were prepared by dissolving 1 g of glucose (dry basis) in 1 L distilled water and diluting it exactly to the desired gradient.…”

Section: Methodsmentioning

confidence: 99%

Stimulation of in situ low intensity ultrasound on batch fermentation of Saccharomyces cerevisiae to enhance the GSH yield

Yang

Xiang

Zhang

et al. 2020

J Food Process Engineering

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In this study, an in situ 7.5 L ultrasonic fermentation tank was used to culture Saccharomyces cerevisiae at 30 C for 36 hr to explore the effects of low intensity ultrasound on the production of glutathione (GSH). Ultrasonic conditions of 28 kHz frequency, 28 W/L power density for fermentation time of 0-12 hr, 280 W/L power density for fermentation time of 12-36 hr, 3 s of interval and fixed frequency mode were used for the setup and a nonsonicated setup served as the control for the experiment. Results showed that ultrasound treatment increased total GSH production by 15.60% from a maximum value of 45.52 mg/L in the control to a maximum value of 52.62 mg/L in the sonicated sample. Also, intracellular and extracellular GSH productions in the sonicated sample increased by 5.60% and 42.92% respectively. Regular detection of the changes of biomass yield, residual sugar, dissolved oxygen and pH showed that sonication resulted in biomass increased by 7.25%, glucose consumption rate increased by 53.08% and dissolved oxygen content decreased by 41.67%. The biomass models of sonication and nonsonication treatments were established according to growth of S. cerevisiae showed that the model fitted well with Logistic regression in which the strain specific growth rate increased by 60.95% after sonication. GSH production model agreed with Luedeking-Piret regression in which GSH synthesis parameters of sonication, α and β increased by 1.96% and 19.64% in comparison to the nonsonicated sample. This study clearly demonstrated that ultrasonic stimulation could improve the biosynthesis of GSH during fermentation. Practical Applications In this study, the GSH was producted by microbial fermentation in in situ 7.5 L ultrasonic fermentation tank. During the fermentation, sonication was applied to increasing yield of GSH. The results show that the study provides a theoretical basis for manufacture GSH combined with sonication industrialization. The fermentation kinetic model was established, which provided experimental basis for continous fermentation.

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Section: Methodsmentioning

confidence: 99%

Stimulation of in situ low intensity ultrasound on batch fermentation of Saccharomyces cerevisiae to enhance the GSH yield

Yang

Xiang

Zhang

et al. 2020

J Food Process Engineering

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“…The glucoamylase consists of a catalytic domain associated with a starch-binding molecule linked with O-glycosylated linker region that vigorously acts upon the partly treated starch molecules to release monomeric form of sugar and resulted in the formation of rough surface of the substrate as compared to the dextrinized sample (Sauer et al 2000;Betiku et al 2013). A similar kind of experiment was also conducted to digest the complex starch molecules by the action of acids and some significant starch hydrolyzing enzymes (Zhang et al 2010;de Souza et al 2019;Strąk-Graczyk and Balcerek 2020).…”

Section: Structural Property Of Treated Sprfmentioning

confidence: 99%

Optimization of saccharification prospective from starch of sweet potato roots through acid-enzyme hydrolysis: structural, chemical and elemental profiling

et al. 2021

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The sweet potato root, a potent source of starch which is being considered as an efficient alternative for fuel ethanol production in recent times. The starchy substrate needs to be subsequently dextrinized and saccharified so as to enhance the utilization of its carbohydrates for ethanol production. In the present investigation, acid-enzyme process was conducted for the dextrinization and saccharification of sweet potato root flour (SPRF). The best optimized condition for dextrinization was achieved with an incubation period of 60 min, temperature 100 ºC and 1M HCl. However, for saccharification, the best result was obtained with an incubation of 18 h, pH 4, temperature 65 ºC and 1000 U concentration of Palkodex®. After the dextrinization process, maximum concentrations of total sugar and hydroxymethylfurfural (HMF) [380.44 ± 3.17 g/kg and 13.28 ± 0.25 mg/g, respectively] were released. Nevertheless, after saccharification, 658.80 ± 7.83 g/kg of total sugar was obtained which was about 73% more than that of dextrinization. After successful dextrinization and saccharification, the structural, chemical and elemental analysis were investigated using techniques such as scanning electron microscopy (SEM), Fourier-transforms infrared spectroscopy (FTIR) and energy-dispersive X-ray fluorescence spectrophotometer (EDXRF), respectively. Effective hydrolysis was demonstrated in thin layer chromatography (TLC) where the HCl was able to generate monomeric sugar such as glucose and maltose. On the other hand, only glucose is synthesized on the mutual effect of HCl and Palkodex®. The SEM findings indicate that the rough structure of both dextrinized and saccharified sample was gained due to the vigorous effect of both acid and enzyme subsequently. The saccharified SPRF when subjected to fermentation with Saccharomyces cerevisiae and Zymomonas mobilis separately, it was observed that Z. mobilis produced more stretching vibration of –OH than S. cerevisiae, which evidenced the better production of bioethanol. Additionally, evaluation of the influence of S. cerevisiae and Z. mobilis through elemental analysis revealed upsurge in the concentrations of S, Cl, Ca, Mn, Fe and Zn and decline in the concentrations of P, K and Cu in the fermented residue of S. cerevisiae and Z. mobilis, however, Z. mobilis showed little more variation than that of S. cerevisiae.

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“…These variations of production may due to the way of acid treatment that was carried out under a higher temperature of 120°C and utilizing mild acid concentration. However, enzymatic treatment leads to complete hydrolysis of the starch component and is highly substrate specific (de Souza, et al, 2019).…”

Section: Enzymatic Hydrolysis Of Ppsmentioning

confidence: 99%

Potato Peels and Mixed Grasses as Raw Materials for Biofuel Production

Omar

Hasan

Lalov

2020

ARO

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Biogas and fuel ethanol are renewable energy sources, can be produced from complex organic materials that are decomposed by microorganisms in the anaerobic digestion method. Potato peels (PPs) and mixed Lolium perenne and Dactylis glomerata grasses were assessed as a potential substrate for biomethanation in a batch method under mesophilic condition (35°C) and ethanol fermentation. The first approach of this work was focused on pretreatment of PPs using acidic and enzymatic hydrolysis to produce biogas and ethanol fermentation using Saccharomyces cerevisiae and Safbrew S-33. These experiments proved that enzymatic hydrolysis produced 1.2 g/L of ethanol involved 115 h of fermentation and 665 ml/h of biogas after 451 h of biomethanation, this was more than the outcomes of acidic treatment. The second approach was concentrated on ability of biogas and ethanol production from mixed grasses treated with different acid concentrations that produced 0.16 g/L ethanol over 8 days of fermentation and 500 ml/h of biogas after 13 days of methanation technique. In general, the results pointed out that PPs and combined grasses can be used as potential substrates with raw materials for biogas and ethanol production.

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Enzymatic hydrolysis of starch into sugars is influenced by microgel assembly

Abstract: Highlights The method of building the microgel influencing sugar reduction. A single-step way to immobilize crude fungal amylase extracts. Microgel as a reusable support of enzymes in starch hydrolysis.

Cited by 13 publications

References 31 publications

Stimulation of in situ low intensity ultrasound on batch fermentation of Saccharomyces cerevisiae to enhance the GSH yield

Stimulation of in situ low intensity ultrasound on batch fermentation of Saccharomyces cerevisiae to enhance the GSH yield

Optimization of saccharification prospective from starch of sweet potato roots through acid-enzyme hydrolysis: structural, chemical and elemental profiling

Potato Peels and Mixed Grasses as Raw Materials for Biofuel Production

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