Ethnic variation in the CYP2E1 gene: polymorphism analysis of 695 African-Americans, European-Americans and Taiwanese

Stephens, Elizabeth; Taylor, Jack A.; Kaplan, Norman; Yang, Chung-Hui; Hsieh, Ling Ling; Lucier, George W.; Bell, Douglas A.

doi:10.1097/00008571-199408000-00002

Cited by 160 publications

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“…However, the distribution of CYP2E1 and ADH 3 genotypes was not significantly different among the disease groups, though the power to detect such differences is low. In addition, frequencies of CYP2E1 C or c2 alleles in our control population (6.4% and 2.3%, respectively) were in agreement with those previously reported in Caucasians (Kato et al, 1992;Lucas et al, 1995;Matthias et al, 1998;Stephens et al, 1994). The ADH 3 allele frequencies among our control subjects (0.57 for ADH 3 1 and 0.43 for ADH 3 2) were also similar to those reported for French subjects (0.55 and 0.45, respectively) .…”

Section: Discussionsupporting

confidence: 93%

“…The CYP2E1 genotypes were also determined by PCR-based methods as described in detail elsewhere (Stephens et al, 1994). Briefly, after the amplification reactions, aliquots (10 to 15 l) were digested for 2 to 3 hr at 37°C with 4 units of Dra1 or Rsa1 restriction enzymes, electrophoresed on ethidium bromide-stained 4% Nusieve agarose gels, and photographed.…”

Section: Genotypingmentioning

confidence: 99%

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Role of alcohol dehydrogenase 3 and cytochrome P‐4502E1 genotypes in susceptibility to cancers of the upper aerodigestive tract

et al. 2000

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Alcohol is a recognized risk factor for upper aerodigestive tract (UAT) cancers, but the mechanism by which alcohol causes cancer remains obscure. Ethanol is oxidized to acetaldehyde (the suspected carcinogenic agent in alcohol) by alcohol dehydrogenases (ADHs) and cytochrome P-4502E1 (CYP2E1), both of which exhibit great inter-individual variability in activity. The hypothesis that these polymorphisms influence susceptibility to alcohol-related cancers remains poorly documented. We investigated whether ADH 3 and CYP2E1 DraI and RsaI genotypes modified the risk of UAT cancers among 121 oral cavity/pharyngeal cancer patients, 129 laryngeal cancer patients, and 172 controls, all French Caucasians. Cancer risks and gene-alcohol interactions were analyzed by unconditional logistic regression, accounting for potential confounders. ADH 3 genotype was not associated with UAT cancer. In contrast, a 2-fold risk of oral cavity/ pharyngeal (OR ‫؍‬ 2.0, 95% CI 1.0 -3.9) and laryngeal (OR ‫؍‬ 1.8, 95% CI 1.0 -3.5) cancers was observed for carriers of the CYP2E1 DraI C variant allele compared with other individuals. The risk associated with the CYP2E1 RsaI c2 variant allele also increased for oral cavity/pharyngeal cancer (OR ‫؍‬ 2.6, 95% CI 1.0 -6.6). The effects of ADH 3 or CYP2E1 genotype and alcohol or tobacco were independent. The highest risk of oral cavity/pharyngeal cancer was observed among the heaviest drinkers (>80 g/day) with the CYP2E1 DraI C allele (OR ‫؍‬ 5.8, 95% CI 1.9 -18.2) or the CYP2E1 RsaI c2 allele (OR ‫؍‬ 7.2, 95% CI 1.4 -38.2) compared with lighter drinkers with other genotypes. Our study suggests that CYP2E1 genotype modifies the risk of UAT cancers, but due to the low frequency of CYP2E1 variant alleles, large-scale studies are needed to confirm our findings. Int.

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Section: Discussionsupporting

confidence: 93%

Section: Genotypingmentioning

confidence: 99%

Role of alcohol dehydrogenase 3 and cytochrome P‐4502E1 genotypes in susceptibility to cancers of the upper aerodigestive tract

et al. 2000

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“…In all populations, the CYP2E1*c2 allele is generally considerably less frequent than the CYP2E1*c1 allele (9). The CYP2E1*c2 alleles are more common among Asians than Caucasians (7).…”

Section: Discussionmentioning

confidence: 99%

“…The results of studies of Asian and European populations show that ADH1B*1 predominated among alcohol addicts and suggest a high risk of alcohol addiction (9,(26)(27)(28)(29). Hence, these investigators emphasize the importance of ADH1B*2 protection against developing alcohol dependence (3,24,(30)(31)(32)(33)(34)(35)(36)(37).…”

Section: Discussionmentioning

confidence: 99%

“…Alcohol metabolism is one of the biological determinants that can significantly influence drinking behavior and the development of alcohol dependence (2). Alcohol dehydrogenase (ADH), aldehyde dehydrogenase and microsomal ethanol-oxidizing system (MEOS/CYP2E1) allele and genotypes occur at different frequencies in particular ethnic groups, and their role in the development of alcohol dependence differs between races and populations (3)(4)(5)(6)(7)(8)(9)(10)(11). These enzymes play a role in the variation in health effect outcomes seen in different populations owing to alcohol consumption.…”

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confidence: 99%

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Genetic polymorphism of alcohol-metabolizing enzyme and alcohol dependence in Polish men

Cichoż‐Lach

Celiński

Wojcierowski

et al. 2010

Braz J Med Biol Res

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Role of alcohol dehydrogenase 3 and cytochrome P-4502E1 genotypes in susceptibility to cancers of the upper aerodigestive tract

Bouchardy¹,

et al. 2000

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Alcohol is a recognized risk factor for upper aerodigestive tract (UAT) cancers, but the mechanism by which alcohol causes cancer remains obscure. Ethanol is oxidized to acetaldehyde (the suspected carcinogenic agent in alcohol) by alcohol dehydrogenases (ADHs) and cytochrome P‐4502E1 (CYP2E1), both of which exhibit great inter‐individual variability in activity. The hypothesis that these polymorphisms influence susceptibility to alcohol‐related cancers remains poorly documented. We investigated whether ADH3 and CYP2E1 DraI and RsaI genotypes modified the risk of UAT cancers among 121 oral cavity/pharyngeal cancer patients, 129 laryngeal cancer patients, and 172 controls, all French Caucasians. Cancer risks and gene–alcohol interactions were analyzed by unconditional logistic regression, accounting for potential confounders. ADH3 genotype was not associated with UAT cancer. In contrast, a 2‐fold risk of oral cavity/pharyngeal (OR = 2.0, 95% CI 1.0–3.9) and laryngeal (OR = 1.8, 95% CI 1.0–3.5) cancers was observed for carriers of the CYP2E1 DraI C variant allele compared with other individuals. The risk associated with the CYP2E1 RsaI c2 variant allele also increased for oral cavity/pharyngeal cancer (OR = 2.6, 95% CI 1.0–6.6). The effects of ADH3 or CYP2E1 genotype and alcohol or tobacco were independent. The highest risk of oral cavity/pharyngeal cancer was observed among the heaviest drinkers (>80 g/day) with the CYP2E1 DraI C allele (OR = 5.8, 95% CI 1.9–18.2) or the CYP2E1 RsaI c2 allele (OR = 7.2, 95% CI 1.4–38.2) compared with lighter drinkers with other genotypes. Our study suggests that CYP2E1 genotype modifies the risk of UAT cancers, but due to the low frequency of CYP2E1 variant alleles, large‐scale studies are needed to confirm our findings. Int. J. Cancer 87:734–740, 2000. © 2000 Wiley‐Liss, Inc.

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Ethnic variation in the CYP2E1 gene: polymorphism analysis of 695 African-Americans, European-Americans and Taiwanese

Cited by 160 publications

References 0 publications

Role of alcohol dehydrogenase 3 and cytochrome P‐4502E1 genotypes in susceptibility to cancers of the upper aerodigestive tract

Role of alcohol dehydrogenase 3 and cytochrome P‐4502E1 genotypes in susceptibility to cancers of the upper aerodigestive tract

Genetic polymorphism of alcohol-metabolizing enzyme and alcohol dependence in Polish men

Role of alcohol dehydrogenase 3 and cytochrome P-4502E1 genotypes in susceptibility to cancers of the upper aerodigestive tract

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