Ethanolic Fermentation Efficiency of Seaweed Solid Waste hydrolysates by Saccharomyces cerevisiae

Abstract

The objective of this research was to determine the saccharification and the fermentation efficiency of seaweed solid waste hydrolysate by Saccharomyces cerevisiae (Accession number 3012.254) in anaerobic condition. The optimum saccharification yield of acid pretreated waste (40.93±1.72)% was obtained after 48 hours with saccharification rate of (0.51±0.02) g/l.h. Higher yield was shown by NaOH pretreated waste (67.29±1.24)% after 24 hours with saccharification rate of (0.81±0.06) g/l.h. The fermentation of enzymatic hydrolysates of acid and alkali pretreated samples by S. cerevisiae produced a maximum of 7.52±0.24 g/l and 14.5±0.54 g/l ethanol respectively after 72 hours fermentation. Maximum ethanol yield was 0.31±0.03 g/g and 0.40±0.02 g/g sugar respectively for acid and alkali pretreated samples. The ethanol yields showed that alkali pretreated sample produces higher conversion substrate ratio (80% of theoretical yield) compared to acid pretreated sample (62% of theoretical yield).

Characteristics of Solid Waste Agar Industries

“…Meanwhile, the waste still contains cellulose (Marinho-Soriano, 2001;Marinho-Soriano & Bourret, 2005) and the amount is approaching 40% (Kumar et al, 2013). The solid waste from agar processing industries in Indonesia (domestic scale) has been explored to be used as a raw material for bioethanol (Anggarawati, 2012;Rahmadini, 2012;Martosuyono, Munifah, & Ningrum, 2016) processed by using strong acids, thermal treatment, commercial enzymes, as well as the addition of the Trichoderma resei, and Saccharomyces cerevisiae (John, Anisha, Nampoothiri, & Pandey, 2011;Kim et al 2011Kim et al , 2012Kim et al , 2013Yanagisawa, Nakamura, Ariga, & Nakasaki, 2011;Ruiz, Jasso, Fernandes, Vicente, & Teixeira, 2013).…”