Ethanolic extract of the Goldenseal, Hydrastis canadensis, has demonstrable chemopreventive effects on HeLa cells in vitro: Drug–DNA interaction with calf thymus DNA as target

Saha, Santu Kumar; Sikdar, Sourav; Mukherjee, Avinaba; Bhadra, Kakali; Boujedaini, Naoual; Khuda‐Bukhsh, Anisur Rahman

doi:10.1016/j.etap.2013.03.023

Cited by 26 publications

(23 citation statements)

References 22 publications

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“…Apoptosis measurement is frequently performed by fluorescence microscopy showing exemplary images of apoptotic bodies and chromatin condensation compared to non-apoptotic control cells (9)(10)(11). Although the underlying cellular processes are characteristic for apoptosis, the methodological evidence is limited by lack of quantification and statistical analysis of a sufficient number of nuclei.…”

Section: Discussionmentioning

confidence: 99%

Detection and Quantification of Nuclear Morphology Changes in Apoptotic Cells by Fluorescence Microscopy and Subsequent Analysis of Visualized Fluorescent Signals

Mandelkow¹,

Gümbel²,

Ahrend³

et al. 2017

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Section: Discussionmentioning

confidence: 99%

Detection and Quantification of Nuclear Morphology Changes in Apoptotic Cells by Fluorescence Microscopy and Subsequent Analysis of Visualized Fluorescent Signals

Mandelkow¹,

Gümbel²,

Ahrend³

et al. 2017

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“…It is reported that saponins exert its cytotoxic activity through apoptosis through signalling pathways to prevent the tumors (Han et al, 2013). DAPI staining is most commonly used assay for observing the apoptosis at DNA level (Saha et al, 2013). In this study, saponins extract induced morphological changes in apoptotic cells which were observed in DAPI staining.…”

Section: Discussionmentioning

confidence: 65%

Effect of crude saponins from Gaultheria trichophylla extract on growth inhibition in human colorectal cancer cells

Alam

Saqib

Waheed

2015

Bangladesh J Pharmacol

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Research ArticleEffect of crude saponins from Effect of crude saponins from Effect of crude saponins from Gaul-GaulGaultheria trichophylla theria trichophylla theria trichophylla extract on extract on extract on growth inhibition in human colorecgrowth inhibition in human colorecgrowth inhibition in human colorectal cancer cells tal cancer cells tal cancer cells BJP IntroductionColorectal cancer is the third most common malignant neoplasm worldwide and has become one of the major causes of cancer mortality (Siegel et al. , 2013). Several studies revealed that loss of control of apoptosis results in initiation of cancer and therefore, many new treatment procedures targeting apoptosis are feasible and may be used to treat various types of cancers (Wong, 2011).It is well known that in the past some medicinally important plants like Catharanthus roseus, Podophyllum peltatum and Taxus brevifolia and others provided therapeutically active metabolites which were used to control the advance stages of malignancies (Farnsworth and Soejarto, 1991). However, most of these chemotherapeutic agents showed untoward effects like mutagenicity and teratogenicity. Therefore, as always, there is need to find alternative drugs with low toxicity, more effective and accessible to common man.The present study was carried out to investigate the anticancer activity of crude extract and respective saponins fraction of Gaultheria trichophylla in cultured human colorectal cancer cells (Caco-2).G. trichophylla belongs to family Ericaceae which consists of 90 genera and over 1,700 species. It is native to Himalayas and commonly known as Himalayan snowberry (Huang, 2003). The flowers range in colour from red, to pink; fruits are blue-coloured berries; and small green leaves are approximately 3-7 mm in length. This plant is an inhabitant of cold and lofty locations of the mountains and like many plants of such locations, it is furnished with setae. The calyx is adherent to the lower part of the capsule, becomes succulent, and forms an edible fruit in the AbstractThe genus Gaultheria also comprised of species with reported cytotoxic activities. Current research work was carried out to evaluate G. trichophylla crude extract and respective saponins fraction against human colorectal cancer cell line (Caco-2) based on cell viability assays. Caco-2 cells treated with the crude extract showed significant growth inhibition (p< 0.001) in a dose dependent manner with apparent IC50 value of 200 μg/mL and 100 μg/ mL in MTT and NRU assays respectively. The fractioned crude saponins showed an enhanced response and inhibited the growth of Caco-2 by 93.6 and 97.4% in MTT and NRU assays respectively, with compared to actinomycin-D (65%). The DAPI staining of cell treated with crude saponins observed under confocal microscope showed shrunken nuclei with apparent nuclear fragmentation and chromatin condensation indicating apoptosis mode of cell death. The study exhibited that the G. Trichophylla saponins induced apoptosis of Caco-2 cell lines. This study provides...

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“…Structurally, the Table III Cytotoxic activity of saponins fractions from Gaultheria trichophylla using NRU assay during the initial stages of necrosis (Nanji and HillerSturmhöfel, 1997). DAPI staining is most commonly used assay for observing the apoptosis at the DNA level (Saha et al, 2013). In this study G. Sa induced morphological changes in apoptotic cells, which were observed in DAPI staining of MCF-7 cells.…”

Section: Discussionmentioning

confidence: 91%

“…After treat-ment, cells were briefly equilibrated with PBS, fixed with 4% paraformaldehyde for 15 min, permeabilized with methanol for 5 min, and mounted in a DAPI-containing medium (Vector Shield, Vector Labs, Peter-borough, UK). The morphology of the nuclei was observed using a confocal fluorescence microscope, Leica SP2 AOBS confocal microscope (Leica Microsystems, Mann-heim, Germany) with excitation at 350 nm and emission 460 nm under a x40 oil objective (Saha et al, 2013).…”

Section: Cytomorphological Alterations (Dapi Staining)mentioning

confidence: 99%

Cytotoxic activity of crude saponins from Gaultheria trichophylla against human breast cancer cells MCF-7 and MDA-MB-468

Alam

Saqib

Waheed

2015

Bangladesh J Pharmacol

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This study was conducted to evaluate Gaultheria trichophylla crude extract and respective saponins fraction against human breast cancer cell lines. In MTT assay, cell viability was inhibited by G. trichophylla crude extract (500 µg/mL) and saponins (200 µg/mL) in a dose dependent manner with maximum inhibition of (82% and 85%) and (71% and 42%) against MCF-7 and MDA MB-468, respectively. In neutral red uptake assay, the cell viability was inhibited by crude extract and saponins (100 µg/mL) in a similar manner with maximum inhibitions of (96% and 93%) and (87% and 61%) against MCF-7 and MDA MB-468, respectively, with respect to 91% and 93% inhibition by actinomycin-D (4 µM). The DAPI (4',6-diamidino-2-phenylindole) (10 µg/mL) staining of MCF-7 cells treated with crude saponins showed shrunken nuclei with apparent nuclear fragmentation indicating apoptosis and in contrast, MDA MB-468 showed necrosis mode of cell death. The study exhibited that the G. trichophylla provides new evidences to further explore this plant for the novel targets in anticancer drug development.

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Ethanolic extract of the Goldenseal, Hydrastis canadensis, has demonstrable chemopreventive effects on HeLa cells in vitro: Drug–DNA interaction with calf thymus DNA as target

Cited by 26 publications

References 22 publications

Detection and Quantification of Nuclear Morphology Changes in Apoptotic Cells by Fluorescence Microscopy and Subsequent Analysis of Visualized Fluorescent Signals

Detection and Quantification of Nuclear Morphology Changes in Apoptotic Cells by Fluorescence Microscopy and Subsequent Analysis of Visualized Fluorescent Signals

Effect of crude saponins from <i>Gaultheria trichophylla</i> extract on growth inhibition in human colorectal cancer cells

Cytotoxic activity of crude saponins from <i>Gaultheria trichophylla</i> against human breast cancer cells MCF-7 and MDA-MB-468

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