2017
DOI: 10.21873/anticanres.11560
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Detection and Quantification of Nuclear Morphology Changes in Apoptotic Cells by Fluorescence Microscopy and Subsequent Analysis of Visualized Fluorescent Signals

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Cited by 77 publications
(65 citation statements)
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“…Interestingly, the TUNEL assay confirmed and expanded the results of nuclear chromatin morphology analysis as statistical significances found on chromatin damage were reproduced on DNA fragmentation. These results allow to confirm the suitability of apoptosis analysis by nuclear chromatin morphology, as reported in other studies (Mandelkow et al, ), in the cumulus oophorus, a numerically limited cellular system consisting of cells aggregated together.…”
Section: Discussionsupporting
confidence: 88%
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“…Interestingly, the TUNEL assay confirmed and expanded the results of nuclear chromatin morphology analysis as statistical significances found on chromatin damage were reproduced on DNA fragmentation. These results allow to confirm the suitability of apoptosis analysis by nuclear chromatin morphology, as reported in other studies (Mandelkow et al, ), in the cumulus oophorus, a numerically limited cellular system consisting of cells aggregated together.…”
Section: Discussionsupporting
confidence: 88%
“…To identify possible mechanisms underlying BEA‐induced reduction of MII rates, the incidence of apoptosis after COC exposure to BEA was investigated. Cell shrinking has been reported among morphological alterations of apoptosis (Mandelkow et al, ). It was found that BEA induces ooplasmic shrinking, even in MII oocytes.…”
Section: Discussionmentioning
confidence: 99%
“…The effect of AuNPs-PEG-RNase A on apoptosis induction was evaluated by DAPI staining assay. 38 Approximately 2×10 3 cells were seeded in 96-well cell culture plate. After incubation at 37°C for 24 hours, the cells were washed 3 times with PBS (NaCl, KCl, KH 2 PO 4 , and Na 2 HPO 4 , pH 7.4).…”
Section: Apoptosis Assay By Dapimentioning
confidence: 99%
“…In U2-OS and MNNG/HOS OS cell lines, CAP treatment activated the apoptosis-specific proteases caspase-3 and caspase-7 [unpublished data]. Later in the apoptotic cascade, cell shrinking has been demonstrated by fluorescence dye stained OS cell nuclei [ 110 ]. Microscopic analysis revealed decreased nuclei measured by a reduced nuclear area and perimeter.…”
Section: Cap Effects On Os Cellsmentioning
confidence: 99%