Ethanol Production from Residual Wood Chips of Cellulose Industry: Acid Pretreatment Investigation, Hemicellulosic Hydrolysate Fermentation, and Remaining Solid Fraction Fermentation by SSF Process

Silva, Neumara Luci Conceição; Betancur, Gabriel Jaime Vargas; Vásquez, Mariana Peñuela; Gomes, Edelvio de Barros; Pereira, Nei

doi:10.1007/s12010-010-9096-8

Cited by 34 publications

(12 citation statements)

References 18 publications

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“…23 Likewise, using hydrothermally pretreated and wet disk milled E. globulus at a solids loading of 35 wt%, Fujii et al achieved ethanol titers of 53.5 g/L. 26 Moreover, our ethanol productivities surpassed the 43.7 g/L (13 wt% solids loading) reported for organosolve pretreated E. globulus 27 and the 28 g/L from H 2 SO 4 pretreated E. grandis wood in high solids loading (20 wt.%) SSF by Silva et al 38 In comparison to previous studies (Table 1), it is evident that high solids loading SSF using SEWC required substantially higher cellulase doses in order to maximize ethanol titers and yields. This is mostly likely due to the fact that the SEWC substrate was not detoxified and was composed of very large, non-uniform particle sizes (that is, a heterogenous mixture of wood, branches, bark, and leaves).…”

Section: Resultsmentioning

confidence: 48%

Simultaneous Saccharification and Fermentation of PretreatedEucalyptus grandisUnder High Solids Loading

McIntosh

Palmer

Zhang

et al. 2017

Industrial Biotechnology

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Whole Eucalyptus grandis trees (including bark, branches and leaves) were investigated as a potential feedstock for bioethanol production. To demonstrate and maximize ethanol production, unwashed steam exploded wood chips (SEWC) were used as substrates in high solids load simultaneous saccharification fermentations (SSF) with an industrial Saccharomyces cerevisiae strain (Fali Ò ). Under optimized SSF conditions-20 wt% solids, 60 filter paper units (FPU)/g glucan, and 36°C-ethanol titer and glucan-to-ethanol yields of 56 g/L and 90%, respectively, were achieved. Raising the SSF temperature to 39°C showed no observable benefit in final ethanol titers and/or yields. Addition of 3 wt% polyethylene glycol (PEG) further increased final ethanol titers and yields to 60 g/L and 95%, respectively, at a 30% lower cellulase dosage. Considering the mass balance of the best-performing SSF (20 wt% SEWC, 40 FPU Ctec 2/g glucan, 30 mg PEG/g dry solid at 36°C) process, the maximum ethanol attainable was estimated at 187 kg (85.8% yield) and 299 kg (95% yield) per dry metric ton of original and SEWC biomass, respectively.

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Section: Resultsmentioning

confidence: 48%

Simultaneous Saccharification and Fermentation of PretreatedEucalyptus grandisUnder High Solids Loading

McIntosh

Palmer

Zhang

et al. 2017

Industrial Biotechnology

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“…Without delignification, up to 70% of cellulases remain immobilized within lignin (Berlin et al, 2005; Jørgensen et al, 2007). This has resulted in development of a variety of pretreatments for eucalypt biomass, to increase efficiency of enzymatic hydrolysis (Yu et al, 2010; Silva et al, 2011; Papa et al, 2012; Santos et al, 2012; Yáñez-S et al, 2013; Zhang C. et al, 2015). Lignin removal also creates pores in the cell wall through which cellulases can gain access to cellulose microfibrils (Yu et al, 2011).…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Relationships between Growth Rate, Tree Size, Lignocellulose Composition, and Enzymatic Saccharification in Interspecific Corymbia Hybrids and Parental Taxa

Healey

Lee

Lupoi³

et al. 2016

Front. Plant Sci.

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In order for a lignocellulosic bioenergy feedstock to be considered sustainable, it must possess a high rate of growth to supply biomass for conversion. Despite the desirability of a fast growth rate for industrial application, it is unclear what effect growth rate has on biomass composition or saccharification. We characterized Klason lignin, glucan, and xylan content with response to growth in Corymbia interspecific F1 hybrid families (HF) and parental species Corymbia torelliana and C. citriodora subspecies variegata and measured the effects on enzymatic hydrolysis from hydrothermally pretreated biomass. Analysis of biomass composition within Corymbia populations found similar amounts of Klason lignin content (19.7–21.3%) among parental and hybrid populations, whereas glucan content was clearly distinguished within C. citriodora subspecies variegata (52%) and HF148 (60%) as compared to other populations (28–38%). Multiple linear regression indicates that biomass composition is significantly impacted by tree size measured at the same age, with Klason lignin content increasing with diameter breast height (DBH) (+0.12% per cm DBH increase), and glucan and xylan typically decreasing per DBH cm increase (-0.7 and -0.3%, respectively). Polysaccharide content within C. citriodora subspecies variegata and HF-148 were not significantly affected by tree size. High-throughput enzymatic saccharification of hydrothermally pretreated biomass found significant differences among Corymbia populations for total glucose production from biomass, with parental Corymbia torelliana and hybrids HF-148 and HF-51 generating the highest amounts of glucose (~180 mg/g biomass, respectively), with HF-51 undergoing the most efficient glucan-to-glucose conversion (74%). Based on growth rate, biomass composition, and further optimization of enzymatic saccharification yield, high production Corymbia hybrid trees are potentially suitable for fast-rotation bioenergy or biomaterial production.

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“…The lignocelluosic biomass is composed of three polymeric fractions: the cellulose, the hemicellulose and the lignin. 1,2 The use of the lignocellulosic biomass to obtain saccharides that can be used for the production of bioethanol, throughout the fermentation process, is a straight focus in the renewable energy area. For this purpose, the cellulose should be hydrolyzed by an enzymatic complex constituted by different enzymes as endo-β(1-4) glucanase (EC 3.2.1.4), exo-β(1-4) glucanase or celobiohydrolase (EC 3.2.1.91) and celobiase or β(1-4) glucosidase (EC 3.2.1.21).…”

Section: Introductionmentioning

confidence: 99%

Potential of the Aspergillus labruscus ITAL 22.223 as a producer of cellulolytic enzymes and xylanase under solid-state fermentation

Maestrello

Guimarães

2018

Int J Sci Rep

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Background: The enzymatic hydrolysis of the lignocellulosic biomass to obtain saccharides that can be used for the production of bioethanol is an important field in the renewable energy area. For this purpose, fungal cellulases and xylanases can be applied.Methods: Aspergillus labruscus ITAL 22.223 was cultured under SSF with agroindustrial residues and by-products as substrates, humidified with different moistening agents, at different proportions (1:0.5, 1:1, 1:1.5 and 1:2; m/v), for different periods (24-216 h) at 25ºC. The extract obtained was used for determination of the cellulase and xylanase activities. The influence of temperature, pH and different compounds on xylanase activity was analyzed. Results: A. labruscus produced cellulases and xylanase under solid-state fermentation (SSF) using agroindustrial by products and residues as carbon source/substrates. The best production of β-glucosidase (6.3 U/g of substrate) was obtained in the presence of rye bran, whereas for the CMCase it was in the presence of crushed soybean (5.1 U/g of substrate) and xylanase using oat bran (74.8 U/g of substrate) as substrates, for 168 h of cultivation at 25ºC. Considering the high xylanase production, the best moistening agent and its proportion (tap water, 1:2 m/v) were determined. Optimum of temperature and pH for xylanase activity was determined as 55ºC and pH 5.5. The xylanase activity was inhibited by different salts, with exception of MnSO4. It was also inhibited by organic solvents, detergents, EDTA, urea and β-mercaptoethanol.Conclusions: The fungus A. labruscus presented potential to produce enzymes from the cellulolytic complex and xylanase using low cost substrates.

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Ethanol Production from Residual Wood Chips of Cellulose Industry: Acid Pretreatment Investigation, Hemicellulosic Hydrolysate Fermentation, and Remaining Solid Fraction Fermentation by SSF Process

Cited by 34 publications

References 18 publications

Simultaneous Saccharification and Fermentation of PretreatedEucalyptus grandisUnder High Solids Loading

Simultaneous Saccharification and Fermentation of PretreatedEucalyptus grandisUnder High Solids Loading

Evaluation of Relationships between Growth Rate, Tree Size, Lignocellulose Composition, and Enzymatic Saccharification in Interspecific Corymbia Hybrids and Parental Taxa

Potential of the Aspergillus labruscus ITAL 22.223 as a producer of cellulolytic enzymes and xylanase under solid-state fermentation

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