Simultaneous Saccharification and Fermentation of Pretreated<i>Eucalyptus grandis</i>Under High Solids Loading

McIntosh, Shane; Palmer, J.; Zhang, Zhanying; Doherty, William O.S.; Yazdani, Syed Shams; Sukumaran, Rajeev; Vancov, Tony

doi:10.1089/ind.2016.0018

Cited by 16 publications

(9 citation statements)

References 50 publications

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“…The results obtained in this work can be positively compared with the ones reported in literature using SO 2 -catalyzed steam pretreated spruce bark, in which 87% of ethanol yield was obtained by separate hydrolysis and fermentation (SHF) (Frankó et al, 2015). The whole Eucalyptus grandis tree (including barks, branches and leaves) was pretreated by steam explosion and used as substrate for ethanol production, achieving an ethanol yield of 90% using 20% of solids and 60 FPU/g (McIntosh et al, 2017). As far as we know, there are no studies for bioethanol production from E. nitens bark using an organosolv process.…”

Section: Simultaneous Saccharification and Fermentation Of Delignifiesupporting

confidence: 52%

Valorization of Eucalyptus nitens bark by organosolv pretreatment for the production of advanced biofuels

Romaní

Larramendi

Yáñez

et al. 2019

Industrial Crops and Products

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The biofuels production from alternative and renewable raw materials is mandatory to achieve sustainable growth based on a bioeconomy. Eucalyptus bark is a waste generated during the chemical manufacturing of Eucalyptus pulp that can be used as an alternative source of biomass, suitable for the production of biofuels. In this work, Eucalyptus nitens bark (ENB) was fractionated by organosolv treatment for ethanol production. For that, a Doehlert experimental design was carried out to evaluate the dependent variables: temperature (170-200°C), time (30-90 min) and ethanol-water percentage (50-80 %) on delignification of Eucalyptus bark. Organosolv process was suitable for the fractionation of E. nitens bark. After treatment, 74-93 % of glucan was recovered and 25-52 % of delignification was achieved. Delignified ENB was subjected to simultaneous saccharification and fermentation process for bioethanol production. The results showed that the variables temperature and time of organosolv process had significant influence on ethanol production. The organosolv pretreatment improved the ethanol yield from 32 to 99%. This work shows a suitable process for the valorization of Eucalyptus bark into bioethanol.

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Section: Simultaneous Saccharification and Fermentation Of Delignifiesupporting

confidence: 52%

Valorization of Eucalyptus nitens bark by organosolv pretreatment for the production of advanced biofuels

Romaní

Larramendi

Yáñez

et al. 2019

Industrial Crops and Products

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“…1 a–e). McIntosh et al. (2017) also reported that the available glucose was rapidly converted to ethanol by the yeast in the first 6–12 h after inoculation in SSF and that 90% of ethanol production occurred during this period.…”

Section: Resultsmentioning

confidence: 88%

Comparison of ethanol yield from pretreated lignocellulo-starch biomass under fed-batch SHF or SSF modes

Mithra

Jeeva

Sajeev

et al. 2018

Heliyon

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The ethanol yields from lignocellulo-starch biomass (peels of sweet potato, elephant foot yam, tannia, greater yam and beet root) by fed-batch separate hydrolysis and fermentation (F-SHF) and simultaneous saccharification and fermentation (F-SSF) using Saccharomyces cerevisiae were compared. Fed-batch saccharification of steam or dilute sulphuric acid pretreated biomass enhanced the reducing sugar yield which resulted in high RS consumption, volumetric ethanol productivity and ethanol yield during the first 24 h fermentation under F-SHF mode, while continuous production and utilization of reducing sugars occurred up to 72 h in F-SSF. Dilute sulphuric acid pretreated residues under F-SHF gave higher ethanol yield (34–43 g/L) and productivity (274–346 ml/kg dry biomass) than steam pretreatment (27–36 g/L and 223–295 ml/kg respectively), while F-SSF was superior for steam pretreated peels of sweet potato, elephant foot yam and tannia giving ethanol yields from 281 to 302 ml/kg. Glucose and xylose were present in all the hydrolysates with a preponderance of glucose and fermentation resulted in significant reduction in glucose levels in both F-SHF and F-SSF. Higher levels of total soluble phenolics and hydroxymethyl furfural were observed in the hydrolysates from dilute sulphuric acid pretreatment and yeast assimilated/detoxified part of the inhibitors, while only trivial amounts of furfural were present due to the low xylose content in the hydrolysates. Continuous formation led to higher accumulation of inhibitors in F-SSF despite supplementation with the detoxification mix comprising Tween 20, polyethylene glycol and sodium borohydride. F-SHF of dilute sulphuric acid pretreated biomass could be considered as a comparatively advantageous process where only one time feeding of enzyme cocktail and yeast was adopted compared to multiple feeds of enzymes and yeast along with other additives such as detoxification mix or nutrient solution in F-SSF.

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“…Rapid conversion of available glucose to ethanol by yeast in the first 6 to12 h after inoculation in SSF was reported by McIntosh et al (2017) who found that 90% of ethanol production occurred during this period. As fed-batch substrate feeding strategy was adopted in the present study, continuous ethanol production was monitored up to 72 h although the rate of conversion of sugars to ethanol was reduced after 24 h. Nguyen et al (2018) obtained high yields of ethanol (33.9 g/L) from soybean residue when galactose adapted yeast was used.…”

Section: Hplc Profile Of Monosaccharidesmentioning

confidence: 60%

Comparison of SHF and SSF Processes under Fed Batch Mode on Ethanol Production from Pretreated Vegetable Processing Residues

Mithra

Sajeev

Padmaja

2019

European Journal of Sustainable Development Research

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Vegetable wastes containing high starch in contrast to lignocellulosic biomass cause environmental threat due to non-judicious disposal and have not been exploited for bioethanol production. The ethanol production from steam or dilute sulphuric acid (DSA) pretreated residues was compared under fed-batch Separate Hydrolysis and Fermentation (F-SHF) or Simultaneous Saccharification and fermentation (F-SSF). The volumetric ethanol productivity, ethanol yields and ethanol contents (g/L) were higher from DSA than steam pretreatment in F-SHF, while latter two did not differ significantly between the pretreatments under F-SSF mode. High RS utilization was observed towards the last phase (72-120 h) in steam pretreatment under F-SSF. Fermented broth from F-SSF had higher levels of inhibitors such as phenolics, furfural and Hydroxymethyl furfural compared to F-SHF and also in the DSA pretreatment. A comparison of the ethanol production from the processes vis-à-vis enzyme and yeast feeding and pretreatment conditions, overall processing time etc. showed that F-SSF had higher requirement of enzymes and yeast than F-SHF. Possibility to curtail fermentation at 24 h under F-SHF mode due to very low ethanol production after 24 h equated the processing time under the two modes to 120 h and this made F-SHF the most advantageous process for the selected residues.

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Simultaneous Saccharification and Fermentation of PretreatedEucalyptus grandisUnder High Solids Loading

Cited by 16 publications

References 50 publications

Valorization of Eucalyptus nitens bark by organosolv pretreatment for the production of advanced biofuels

Valorization of Eucalyptus nitens bark by organosolv pretreatment for the production of advanced biofuels

Comparison of ethanol yield from pretreated lignocellulo-starch biomass under fed-batch SHF or SSF modes

Comparison of SHF and SSF Processes under Fed Batch Mode on Ethanol Production from Pretreated Vegetable Processing Residues

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