Ethanol production from non-starch carbohydrates of wheat bran

Palmarola-Adrados, Beatriz; Chotěborská, Pavla; Galbe, Mats; Zacchi, Guido

doi:10.1016/j.biortech.2004.07.004

Cited by 150 publications

(74 citation statements)

References 9 publications

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“…AMG is an enzyme that acts from the non-reducing end and it is used to terminate the starch saccharification cleaving both α1,4 and α1,6 bonds (Palmarola-Adrados, Chotěborská, Galbe, & Zacchi, 2005). Therefore, the concentration and the time of action of these enzymes are of extreme importance for the complete starch hydrolysis.…”

Section: Results and Discussion Enzymatic Hydrolysis Of Drbmentioning

confidence: 99%

“…Palmarola-Adrados, Chotěborská, Galbe, & Zacchi, 2005)., the enzyme reaction speed increases since the substrate is in excess, justifying the need to shorten the action time of AMG, taking in to account the range studied for the DRB dilution rate. For the concentration of both enzymes, the effect was positive and significant (p ≤ 0.10), evidencing an incomplete starch hydrolysis, requiring an increase in the concentration of both enzymes.…”

Section: Results and Discussion Enzymatic Hydrolysis Of Drbmentioning

confidence: 99%

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<b>Release optimization of fermentable sugars from defatted rice bran for bioethanol production

et al. 2018

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ABSTRACT. The aim of this study was to optimize the enzymatic hydrolysis of defatted rice bran (DRB) for release fermentable sugars with subsequent bioethanol production. Optimization of enzymatic hydrolysis was achieved using a sequential experimental design, performing a fractional factorial design of amyloglucosidase (AMG), at action times of two and three hours, respectively. In bioethanol production, it was evaluated the effect of adding of 15 μL g -1 of protease prior to the action of amylases. Fermentation was conducted at 29°C, with 4.0% Saccharomyces cerevisiae and initial pH of 5.0. The hydrolysis with protease yielded 3.55% ethanol, an increase of 115.15% in relation to the medium without protease addition, and the conversion had a yield of 101.5% in 24h of alcoholic fermentation. This study demonstrates the possibility to obtain bioethanol from DRB.

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Section: Results and Discussion Enzymatic Hydrolysis Of Drbmentioning

confidence: 99%

Section: Results and Discussion Enzymatic Hydrolysis Of Drbmentioning

confidence: 99%

<b>Release optimization of fermentable sugars from defatted rice bran for bioethanol production

et al. 2018

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“…Then the mixture was added to 50 mL of the above solution with 250 mL of distilled water. Absorbance readings were taken at 540 nm, with one unit of enzyme activity (IU) corresponding to 1 mol of glucose released per 123 mg of enzyme per minute during hydrolysis (Adrado et al 2005).…”

Section: Quantitative Determination Of Endoglucanase Activity In the mentioning

confidence: 99%

Quantification of Endoglucanase Activity based on Carboxymethyl Cellulose in Four Fungi Isolated from an Aerated Lagoon in a Pulp and Paper Mill

Ordaz-Díaz¹,

Rojas‐Contreras

Flores-Vichi³

et al. 2016

BioResources

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The aim of this study was to identify cellulolytic fungal strains capable of degrading cellulose from an aerated lagoon in a pulp and paper mill. Four fungal strains that were found to be highly active were isolated on carboxymethyl cellulose (CMC) and suggested to be CMCase/endoglucanase. The identified strains were Aspergillus niger, Penicillium sp., Aspergillus fumigatus, and Mucor sp. All the strains were studied in terms of cultural morphological characteristics and microscopic examinations. The endoglucanase with the highest isolate production was Penicillium sp., which also showed the highest qualitative endoglucanase activity (1.3 cm), in addition to the main activity of endoglucanase with 297 mmol/mg.min after 116 h. The results indicated that CMC is able to induce endoglucanase enzyme production and that the fungal isolates showed significant cellulose degradation properties.

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“…Twenty milliliters of supernatant was transferred to 100 ml serum bottles for fermentation. The hydrolyzate was then adjusted to pH 7 by adding 2 N NaOH and inoculated with S. cerevisiae at a cell concentration of 10 g dry matter/l [20]. All samples were then incubated at 30±1°C for 72 h. Liquid samples were analyzed for ethanol content after fermentation.…”

Section: Fermentationmentioning

confidence: 99%

Potential of Agricultural Residues and Hay for Bioethanol Production

Chen

Sharma-Shivappa

Keshwani

et al. 2007

Appl Biochem Biotechnol

157

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Production of bioethanol from agricultural residues and hays (wheat, barley, and triticale straws, and barley, triticale, pearl millet, and sweet sorghum hays) through a series of chemical pretreatment, enzymatic hydrolysis, and fermentation processes was investigated in this study. Composition analysis suggested that the agricultural straws and hays studied contained approximately 28.62-38.58% glucan, 11.19-20.78% xylan, and 22.01-27.57% lignin, making them good candidates for bioethanol production. Chemical pretreatment with sulfuric acid or sodium hydroxide at concentrations of 0.5, 1.0, and 2.0% indicated that concentration and treatment agent play a significant role during pretreatment. After 2.0% sulfuric acid pretreatment at 121°C/15 psi for 60 min, 78.10-81.27% of the xylan in untreated feedstocks was solubilized, while 75.09-84.52% of the lignin was reduced after 2.0% sodium hydroxide pretreatment under similar conditions. Enzymatic hydrolysis of chemically pretreated (2.0% NaOH or H 2 SO 4 ) solids with Celluclast 1.5 LNovozym 188 (cellobiase) enzyme combination resulted in equal or higher glucan and xylan conversion than with Spezyme® CP-xylanase combination. The glucan and xylan conversions during hydrolysis with Celluclast 1.5 L-cellobiase at 40 FPU/g glucan were 78.09 to 100.36% and 74.03 to 84.89%, respectively. Increasing the enzyme loading from 40 to 60 FPU/g glucan did not significantly increase sugar yield. The ethanol yield after fermentation of the hydrolyzate from different feedstocks with Saccharomyces cerevisiae ranged from 0.27 to 0.34 g/g glucose or 52.00-65.82% of the theoretical maximum ethanol yield.

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Ethanol production from non-starch carbohydrates of wheat bran

Cited by 150 publications

References 9 publications

<b>Release optimization of fermentable sugars from defatted rice bran for bioethanol production

<b>Release optimization of fermentable sugars from defatted rice bran for bioethanol production

Quantification of Endoglucanase Activity based on Carboxymethyl Cellulose in Four Fungi Isolated from an Aerated Lagoon in a Pulp and Paper Mill

Potential of Agricultural Residues and Hay for Bioethanol Production

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