Ethanol production at high temperature from cassava pulp by a newly isolated &lt;em&gt;Kluyveromyces marxianus&lt;/em&gt; strain, TISTR 5925

Apiwatanapiwat, Waraporn; Rugthaworn, Prapassorn; Vaithanomsat, Pilanee; Thanapase, Warunee; Kosugi, Akihiko; Arai, T.; Mori, Yutaka; Murata, Yoshinori

doi:10.3934/energy.2013.1.3

Cited by 17 publications

(9 citation statements)

References 13 publications

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“…However, the low ethanol concentration obtained might be due to the inefficiency of raw starch hydrolysis at 42°C, which works with 30% of the maximum activity at the optimum temperature of 50°C . Apiwatanapiwat et al reported that 50 g/L ethanol was obtained from 20% cooked cassava pulp by the simultaneous saccharification and fermentation process using K. marxianus TISTR 5925 at 41°C for 28 h.…”

Section: Resultsmentioning

confidence: 99%

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Production of raw starch degrading enzyme by the thermophilic filamentous bacterium Laceyella sacchari LP175 and its application for ethanol production from dried cassava chips

Lertwattanasakul

Kitpreechavanich

2016

Starch Stärke

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Physical factors that enhanced the production of raw starch degrading enzyme by a thermophilic filamentous bacterium, Laceyella sacchari LP175 in a 3 L airlift fermenter, were optimized at a pH of 6.5, a temperature of 45°C, and an aeration rate of 0.5 vvm using a central composite design. This yielded 278 U/mL, at 36 h cultivation, an increase in enzyme production and productivity of 1.53‐ and 2.04‐fold, respectively, as compared with a shaking flask cultivation. Raw starch degrading enzyme produced by L. sacchari LP175 with commercial glucoamylase synergistically enhanced the hydrolysis of 300 g/L dried cassava chips (71% starch content) to 157 g/L reducing sugars, equivalent to 66.3% hydrolysis at pH 6.5 and 50°C for 12 h. The comparative study on bioethanol production in a non‐sterile system from dried cassava chip by three different processes; the maximum production, 90.9 g/L (88% theoretical yield), was obtained by modified simultaneous saccharification and fermentation at 50°C for 6 h and subsequent fermentation by the thermotolerant Kluyveromyces marxianus DMKU‐KS07 at 42°C within 18 h with the highest productivity of 3.79 g/L/h. The other processes tested – separate hydrolysis and fermentation and simultaneous saccharification and fermentation – yielded 83 and 51.6 g/L of ethanol, respectively.

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Section: Resultsmentioning

confidence: 99%

“…Ethanol concentration was analyzed by gas chromatography (GC4000; GL Sciences, Tokyo, Japan) using a glass column (Chromosorb 103, 60/80 mesh, ID 3 mm × 3 m; Shinwa Chemical Industries, Kyoto, Japan) with a flame ionization detector and n ‐propanol as an internal standard .…”

Section: Methodsmentioning

confidence: 99%

Production of raw starch degrading enzyme by the thermophilic filamentous bacterium Laceyella sacchari LP175 and its application for ethanol production from dried cassava chips

Lertwattanasakul

Kitpreechavanich

2016

Starch Stärke

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“…The optimal temperatures for most of the carbohydrate hydrolyzing enzymes are usually higher than the optimal temperatures for the conventional yeast strains used to convert the products of hydrolysis to ethanol. Although conversion of cassava pulp to ethanol can be done in two sequential steps of hydrolysis followed by fermentation, many reports have demonstrated that simultaneous hydrolysis and fermentation leads to higher productivities and yields [14]. However, the use of Simultaneous Saccharification and Fermentation process requires a yeast strain capable of growing, fermenting sugars as well as producing high concentration of ethanol at temperatures close to the temperature of hydrolysis [15].…”

Section: Introductionmentioning

confidence: 99%

“…Also, the use of thermo-tolerant yeast will save cost of cooling especially in tropical climates where ambient temperature can easily exceed 40˚C [16]. There have been documentations on thermotolerant yeast strains capable of producing ethanol at a temperature up to 42˚C [14] is need to screen for more thermotolerant yeast strains for industrial production of bioethanol. The aim of this work is to screen for thermotolerant ethanol fermenting yeasts and evaluate their potentials for bioethanol production from cassava pulp.…”

Section: Introductionmentioning

confidence: 99%

Ethanol Production from Cassava Pulp by a Newly Isolated Thermotolerant <i>Pichia kudriavzevii</i> LC375240

Ndubuisi¹,

Nweze²,

Onoyima³

et al. 2018

EPE

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A total of 500 thermotolerant fermenting yeast isolates (100 from palm-wine and 400 from spoilt fruits) were screened for ethanol production at high temperatures. The best isolate that produced up to 4% ethanol from 10% glucose at 45˚C was selected for further experiments. The optimum pH for ethanol production by the isolate was pH 6 at both 30˚C and 42˚C. The isolate was identified as Pichia kudriavzevii base on the 18s ribosomal DNA. Ethanol production from 200 g/L cassava pulp using Simultaneous Saccharification and Fermentation (SSF) method at 30˚C and 42˚C by the isolate was investigated. At 30˚C, an ethanol concentration of 30 g/L was produced. This represents an ethanol yield of 0.15 g/g of cassava pulp and 58.8% of the theoretical yield. However at 42˚C, the concentration of ethanol produced increased to 42 g/L representing an ethanol yield of 0.21 g/g of cassava pulp and 82.4% of the theoretical yield. The isolate produced slightly higher ethanol than the two typed strains NCYC 587 and NCYC 2791 at 42˚C. This isolate has a good potential to be used for commercial bioethanol production since it can produce ethanol at 45˚C without a significant drop in ethanol yield.

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“…They reported that SSF gave a 13% higher overall ethanol yield than SHF (72.4% versus 59.1% of the theoretical). In addition, Apiwatanapiwat et al (2013) also found that the ethanol concentration produced from cassava pulp using the SSF process was higher than that using the SHF process. Combination of the two process steps also results in a lower capital cost, the reduction of the requirements for many reactors and the fact that the ethanol concentration is higher during SSF than SHF reduces the risk of contamination (Adekunle et al, 2016).…”

Section: Ethanol Fermentationmentioning

confidence: 81%

Ethanol production from cellulosic cassava waste

Rawaengsungnoen¹,

Leklai²,

Tantipaibulvut³

2018

j.appsci.

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Cassava cellulosic waste obtained from starch processing was utilized in this work for bio-ethanol production. The 10% (w/v) of cassava waste was pretreated with either water or 1% (v/v) acetic acid and steam treated by autoclaving at 121 °C under pressure of 15 pound/inch 2 for 15 min. After neutralization of the acid-autoclaved pretreated waste with 2% (w/v) NaHCO 3 , the mixture was hydrolyzed further by using 3% or 6% (w/w) cellulase for sugar production. It appeared that the optimum hydrolysis condition was 1% (v/v) acetic acid and 6% (w/w) enzyme (shaking at 50 °C, 150 rpm for 96 h), which gave the maximum sugar yield of 199.82 ± 0.27 mg/g dried cellulosic waste. A total of 515.39 ± 0.48 mg ethanol/g dried cellulosic waste was obtained from 9% (w/v) of Saccharomyces cerevisiae in 7-days fermentation at room temperature. This indicates that cassava wastes actually could be used as substrate for biochemical production, such as fuels, organic acids and etc. In addition, the pretreatment method using dilute acetic acid and steam is environmentally friendly and not complex.

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Ethanol production at high temperature from cassava pulp by a newly isolated <em>Kluyveromyces marxianus</em> strain, TISTR 5925

Cited by 17 publications

References 13 publications

Production of raw starch degrading enzyme by the thermophilic filamentous bacterium Laceyella sacchari LP175 and its application for ethanol production from dried cassava chips

Production of raw starch degrading enzyme by the thermophilic filamentous bacterium Laceyella sacchari LP175 and its application for ethanol production from dried cassava chips

Ethanol Production from Cassava Pulp by a Newly Isolated Thermotolerant <i>Pichia kudriavzevii</i> LC375240

Ethanol production from cellulosic cassava waste

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Ethanol production at high temperature from cassava pulp by a newly isolated <em>Kluyveromyces marxianus</em> strain, TISTR 5925

Cited by 17 publications

References 13 publications

Production of raw starch degrading enzyme by the thermophilic filamentous bacterium Laceyella sacchari LP175 and its application for ethanol production from dried cassava chips

Production of raw starch degrading enzyme by the thermophilic filamentous bacterium Laceyella sacchari LP175 and its application for ethanol production from dried cassava chips

Ethanol Production from Cassava Pulp by a Newly Isolated Thermotolerant &lt;i&gt;Pichia kudriavzevii&lt;/i&gt; LC375240

Ethanol production from cellulosic cassava waste

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Ethanol Production from Cassava Pulp by a Newly Isolated Thermotolerant <i>Pichia kudriavzevii</i> LC375240