Ethanol Production from Cassava Pulp by a Newly Isolated Thermotolerant &amp;lt;i&amp;gt;Pichia kudriavzevii&amp;lt;/i&amp;gt; LC375240

Ndubuisi, Ifeanyi A.; Nweze, Julius Eyiuche; Onoyima, Nnaemeka J.; Murata, Yoshinori; Ogbonna, James C.

doi:10.4236/epe.2018.1010029

Cited by 15 publications

(16 citation statements)

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“…Such conditions trigger a series of biological responses to sustain cell cycle progression and growth, cell division and cell proliferation (Mager and Ferreira 1993). Recently, a number of reports have highlighted P. kudriavzevii as an effective thermotolerant yeast strain for the fermentation of bioethanol from sugarcane juice (Dhaliwal et al 2011), sweet sorghum juice (Techaparin et al 2017) and cassava pulp (Ndubuisi et al 2018). Our thermotolerance data were consistent with the previous literature, with other P. kudriavzevii strains being reported to grow at temperatures above 40°C (Sirisan et al 2013;Costa et al 2014;Choi et al 2017;D ıaz-Nava et al 2017).…”

Section: Resultssupporting

confidence: 90%

“…A high ethanol concentration of 33Á84 g l -1 at 40°C was shown by P. kudriavzevii RZ8-1 (Chamnipa et al 2018), while P. kudriavzevii KKU-TH33 and KKU-TH43 show heat and ethanol stress tolerance (Techaparin et al 2017). P. kudriavzevii also demonstrated other characteristics suitable for industrial processes, such as the ability to utilize a wide variety of substrates including hexose, pentose sugar or food waste (Koutinas et al 2016;Ndubuisi et al 2018). The potential application of various non-Saccharomyces yeasts has also been explored for bioethanol fermentation.…”

Section: Introductionmentioning

confidence: 99%

“…2016; Ndubuisi et al . 2018). The potential application of various non‐ Saccharomyces yeasts has also been explored for bioethanol fermentation.…”

Section: Introductionmentioning

confidence: 99%

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The ability of Pichia kudriavzevii to tolerate multiple stresses makes it promising for developing improved bioethanol production processes

Pongcharoen

2022

Letters in Applied Microbiology

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Thermotolerant ethanol fermenting yeasts have been extensively used in industrial bioethanol production. However, little is known about yeast physiology under stress during bioethanol processing. This study investigated the physiological characteristics of the thermotolerant yeast Pichia kudriavzevii, strains NUNS‐4, NUNS‐5 and NUNS‐6, under the multiple stresses of heat, ethanol and sodium chloride. Results showed that NUNS‐4, NUNS‐5 and NUNS‐6 displayed higher growth rates under each stress condition than the reference strain, Saccharomyces cerevisiae TISTR5606. Maximum specific growth rates under stresses of heat (45°C), 15% v/v ethanol and 1·0 M sodium chloride were 0·23 ± 0·04 (NUNS‐4), 0·11 ± 0·01 (NUNS‐5) and 0·15 ± 0·01 h–1 (NUNS‐5), respectively. Morphological features of all yeast studied changed distinctly with the production of granules and vacuoles when exposed to ethanol, and cells were elongated under increased sodium chloride concentration. This study suggests that the three P. kudriavzevii strains are potential candidates to use in industrial–scale fermentation due to a high specific growth rate under multiple stress conditions. Multiple stress‐tolerant P. kudriavzevii NUNS strains have received much attention not only for improving large‐scale fuel ethanol production, but also for utilizing these strains in other biotechnological industries.

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Section: Resultssupporting

confidence: 90%

Section: Introductionmentioning

confidence: 99%

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The ability of Pichia kudriavzevii to tolerate multiple stresses makes it promising for developing improved bioethanol production processes

Pongcharoen

2022

Letters in Applied Microbiology

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“…Majority of the agricultural wastes are lignocellulosic materials which are abundant and cheap biomass resources f or production of useful metabolites (Edama et al, 2014;Kongkiattikajorn, 2012;Pooja and Padmaja,2014). Thus, they have been used as carbon sources f or production of various products such as single cell protein, industrial enzyme, and f ine chemicals (Mohammed et al, 2013;Ndubuisi et al, 2018;Murata, et al, 2021). However, since cellulose, and starch are the major components of most of these agricultural wastes, their ef f icient utilization requires the use of both cellulase and amylase enzymes.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous production of cellulase and amylase by Aspergillus fumigatus IB-A1

Ezea¹,

Murata²,

Ogbonna³

2022

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Utilization of agricultural wastes for production of useful metabolites requires hydrolysis using both cellulase and amylase enzymes. We isolated Aspergillus fumigatus IB-A1 and evaluated its ability to simultaneously produce both cellulase and amylase. Although the isolate could produce both cellulase and amylase from either soluble starch or carboxymethyl cellulose, amylase activity was higher with soluble starch while cellulase activity was higher when carboxymethyl cellulose was used as the sole carbon source. With a mixture of carboxymethyl cellulose and soluble starch, both the amylase and cellulase activities increased with increase in the ratio of soluble starch. The optima ratio of carboxymethyl cellulose to soluble starch for cellulase and amylase activities were 0.7:0.3, and 0.4 to 0.6 respectively. For practical application, the optimum ratio of carboxymethyl cellulose to soluble starch in the production medium depends on the relative composition of cellulose and starch in the substrate to be hydrolyzed. The isolate was also able to efficiently produce both amylase and cellulase from cassava peel. With 10 g/L cassava peel, the cellulase and amylase activities were 6.122± 0.320 U/ml/min and 4.342± 0.210 U/ml/min respectively. When the cells were immobilized on loofa sponge and subjected to alternating air phase- liquid phase culture, cellulase and amylase production from cassava peel increased to 8.106± 0.620 U/ml/min and 5.206± 1.24 U/ml/min respectively. The optimum ratio of the air phase to the liquid phase was 3 hours of air phase to 21 hours of liquid phase.

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“…However, to produce it at levels that could be maintained ad infinitum to meet world demand, many hurdles still have to be crossed. Among many others is the fact that at present, its cost of production is much higher than those of fossil fuels; additionally, many food crops are required to produce it at large commercial scales currently [4]. Sugarcane which contains the necessary sucrose feedstock for its production is the major raw material mostly used, although other starchy materials like cassava and corn are increasingly been utilized also [5].…”

Section: Introductionmentioning

confidence: 99%

Studies on Bioethanol Production with Thermo Tolerant Yeast Isolates and their Co-Cultures using African Wild Cocoyam as Feedstock

Chukwudi

OkeChukwu

Ifeanyi

et al. 2021

AJB2T

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In this work different ways of optimally producing bioethanol at various pH with thermotolerant yeasts and their cocultures using a non-human edible starchy food as feedstock was examined. African wild cocoyam, Xanthosoma roseum, sourced from abandoned farmlands in Obukpa, Nsukka, Nigeria was used as the substrate, while strains of Kluyveromyces marxianus and Pichia stipitis were used to ferment them. First the tubers were gelatinized by boiling under pressure above 100oC before hydrolysis with concentrated H2SO4. The hydrolysates were then fermented at 35oC with the thermotolerant yeasts for five days at different pH. Results obtained showed that gelatinized sample of the substrate gave optimum glucose yield when hydrolysed with 1M H2SO4 for 60 minutes. Kluyveromyces marxianus produced more ethanol than Pichia stipitis at all the four fermentation pH values tested. However, optimum ethanol production was obtained when the two yeast strains were used as coculture at pH 4.5. The peak time for ethanol production was 96 hours for the individual yeast cultures while that of their coculture was 72 hours. The results of the study indicated that wild cocoyam is an excellent feedstock for bioethanol production with many advantages including being non-edible, thereby eliminating concerns for food security, and containing high amount of carbohydrate. The study also revealed that fermenting sugar hydrolysates with a coculture of microorganisms during bioethanol production is a more efficient process than using individual cultures.

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Ethanol Production from Cassava Pulp by a Newly Isolated Thermotolerant <i>Pichia kudriavzevii</i> LC375240

Cited by 15 publications

References 29 publications

The ability of Pichia kudriavzevii to tolerate multiple stresses makes it promising for developing improved bioethanol production processes

The ability of Pichia kudriavzevii to tolerate multiple stresses makes it promising for developing improved bioethanol production processes

Simultaneous production of cellulase and amylase by Aspergillus fumigatus IB-A1

Studies on Bioethanol Production with Thermo Tolerant Yeast Isolates and their Co-Cultures using African Wild Cocoyam as Feedstock

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