Ethanol fixed brain imaging by phase-contrast X-ray technique

Takeda, Takeshi; Thet-Thet-Lwin,; Kunii, Takuya; Sirai, Ryota; Ohizumi, Takahito; Maruyama, Hiroko; Hyodo, Kazuyuki; Yoneyama, Akio; Ueda, Kazuhiro

doi:10.1088/1742-6596/425/2/022004

Cited by 10 publications

(9 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…S2 , formaldehyde reduces contrast and was therefore discarded: the muscular fibrous networks of the vocalis now appear as a fully homogeneous material. On the contrary, the use of ethanol solutions improve contrast, in agreement with the 3D images obtained in previous studies 28 , 35 , 36 . After a 3-day immersion in an aqueous solution with 30% of ethanol (Supplementary Fig.…”

Section: Optimisation Of X-ray Imaging Conditionssupporting

confidence: 91%

3D multiscale imaging of human vocal folds using synchrotron X-ray microtomography in phase retrieval mode

Bailly¹,

Cochereau²,

Orgéas³

et al. 2018

Sci Rep

View full text Add to dashboard Cite

Human vocal folds possess outstanding abilities to endure large, reversible deformations and to vibrate up to more than thousand cycles per second. This unique performance mainly results from their complex specific 3D and multiscale structure, which is very difficult to investigate experimentally and still presents challenges using either confocal microscopy, MRI or X-ray microtomography in absorption mode. To circumvent these difficulties, we used high-resolution synchrotron X-ray microtomography with phase retrieval and report the first ex vivo 3D images of human vocal-fold tissues at multiple scales. Various relevant descriptors of structure were extracted from the images: geometry of vocal folds at rest or in a stretched phonatory-like position, shape and size of their layered fibrous architectures, orientation, shape and size of the muscle fibres as well as the set of collagen and elastin fibre bundles constituting these layers. The developed methodology opens a promising insight into voice biomechanics, which will allow further assessment of the micromechanics of the vocal folds and their vibratory properties. This will then provide valuable guidelines for the design of new mimetic biomaterials for the next generation of artificial larynges.

show abstract

Section: Optimisation Of X-ray Imaging Conditionssupporting

confidence: 91%

3D multiscale imaging of human vocal folds using synchrotron X-ray microtomography in phase retrieval mode

Bailly¹,

Cochereau²,

Orgéas³

et al. 2018

Sci Rep

View full text Add to dashboard Cite

show abstract

“…Very good soft tissue contrast together with non-destructive volumetric information can be provided also by highly sophisticated methods such as X-ray phase-contrast 23 24 25 26 27 28 , dark-field imaging 29 30 or X-ray microscopy 31 32 . These methods, however, require an advanced setup and very special X-ray beam characteristics, which in most of the cases can be found only at large scale synchrotron facilities.…”

Section: Discussionmentioning

confidence: 99%

“…In this work we demonstrate performance of this recently developed large area hybrid pixel photon counting detector for high resolution X-ray microradiography and micro-tomography of ex-vivo ethanol-only preserved murine organs. The effect of ex-vivo soft tissue contrast enhancement after ethanol-preservation has been demonstrated in studies related to phase contrast X-ray imaging 23 24 . However, in the case of techniques based on X-ray absorption, ethanol is generally used as a soft tissue fixative and the sample needs to be further stained by a contrast agent (Lugol solution, phosphotungstic acid, osmium tetroxide, etc.)…”

Section: Photon Counting Detector Technologymentioning

confidence: 99%

High-contrast X-ray micro-radiography and micro-CT of ex-vivo soft tissue murine organs utilizing ethanol fixation and large area photon-counting detector

Dudák

Žemlička

Karch

et al. 2016

Sci Rep

View full text Add to dashboard Cite

Using dedicated contrast agents high-quality X-ray imaging of soft tissue structures with isotropic micrometre resolution has become feasible. This technique is frequently titled as virtual histology as it allows production of slices of tissue without destroying the sample. The use of contrast agents is, however, often an irreversible time-consuming procedure and despite the non-destructive principle of X-ray imaging, the sample is usually no longer usable for other research methods. In this work we present the application of recently developed large-area photon counting detector for high resolution X-ray micro-radiography and micro-tomography of whole ex-vivo ethanol-preserved mouse organs. The photon counting detectors provide dark-current-free quantum-counting operation enabling acquisition of data with virtually unlimited contrast-to-noise ratio (CNR). Thanks to the very high CNR even ethanol-only preserved soft-tissue samples without addition of any contrast agent can be visualized in great detail. As ethanol preservation is one of the standard steps of tissue fixation for histology, the presented method can open a way for widespread use of micro-CT with all its advantages for routine 3D non-destructive soft-tissue visualisation.

show abstract

“…As propagation-based imaging is sensitive to relative changes in electron density, a medium with significantly smaller density with respect to the tissue of interest can lead to superior contrast in the reconstructed images [41,69,84,161].…”

Section: Embedding Mediamentioning

confidence: 99%

3d virtual histology of neuronal tissue by propagation-based x-ray phase-contrast tomography

Töpperwien¹

2018

Göttingen Series in X-Ray Physics

View full text Add to dashboard Cite

Ethanol fixed brain imaging by phase-contrast X-ray technique

Cited by 10 publications

References 18 publications

3D multiscale imaging of human vocal folds using synchrotron X-ray microtomography in phase retrieval mode

3D multiscale imaging of human vocal folds using synchrotron X-ray microtomography in phase retrieval mode

High-contrast X-ray micro-radiography and micro-CT of ex-vivo soft tissue murine organs utilizing ethanol fixation and large area photon-counting detector

3d virtual histology of neuronal tissue by propagation-based x-ray phase-contrast tomography

Contact Info

Product

Resources

About