3D multiscale imaging of human vocal folds using synchrotron X-ray microtomography in phase retrieval mode

Bailly, Lucie; Cochereau, Thibaud; Orgéas, Laurent; Bernardoni, Nathalie Henrich; Roscoat, Sabine Rolland Du; McLeer-Florin, Anne; Robert, Y.; Laval, Xavier; Laurencin, T.; Chaffanjon, Philippe; Fayard, Barbara; Boller, Élodie

doi:10.1038/s41598-018-31849-w

Cited by 31 publications

(37 citation statements)

References 66 publications

(89 reference statements)

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“…Recently, synchrotron X-ray microtomography have been used successfully on human cadavers 16 , and nanotomography on rabbits 17 to identify the tri-dimensional structures of the VF ECM, including its muscle fibers morphology. However, these techniques are not collagen or elastin sensitive and suffer from low contrast since they instead rely on tissue density (X-ray attenuation coefficient) as a contrast mechanism.…”

Section: Introductionmentioning

confidence: 99%

Quantitative evaluation of the human vocal fold extracellular matrix using multiphoton microscopy and optical coherence tomography

Benboujja

Hartnick

2021

Sci Rep

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Identifying distinct normal extracellular matrix (ECM) features from pathology is of the upmost clinical importance for laryngeal diagnostics and therapy. Despite remarkable histological contributions, our understanding of the vocal fold (VF) physiology remains murky. The emerging field of non-invasive 3D optical imaging may be well-suited to unravel the complexity of the VF microanatomy. This study focused on characterizing the entire VF ECM in length and depth with optical imaging. A quantitative morphometric evaluation of the human vocal fold lamina propria using two-photon excitation fluorescence (TPEF), second harmonic generation (SHG), and optical coherence tomography (OCT) was investigated. Fibrillar morphological features, such as fiber diameter, orientation, anisotropy, waviness and second-order statistics features were evaluated and compared according to their spatial distribution. The evidence acquired in this study suggests that the VF ECM is not a strict discrete three-layer structure as traditionally described but instead a continuous assembly of different fibrillar arrangement anchored by predominant collagen transitions zones. We demonstrated that the ECM composition is distinct and markedly thinned in the anterior one-third of itself, which may play a role in the development of some laryngeal diseases. We further examined and extracted the relationship between OCT and multiphoton imaging, promoting correspondences that could lead to accurate 3D mapping of the VF architecture in real-time during phonosurgeries. As miniaturization of optical probes is consistently improving, a clinical translation of OCT imaging and multiphoton imaging, with valuable qualitative and quantitative features, may have significant implications for treating voice disorders.

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Section: Introductionmentioning

confidence: 99%

Quantitative evaluation of the human vocal fold extracellular matrix using multiphoton microscopy and optical coherence tomography

Benboujja

Hartnick

2021

Sci Rep

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“…The water content of the specimens in future tests could be controlled by raising the relative humidity of the air within the specimen chamber to nearly 100 % using a humidifier. [46] Tensile behavior…”

Section: The Effect Of Water Contentmentioning

confidence: 99%

Gaining Insight into the Deformation of Achilles Tendon Entheses in Mice

Sartori

Koehring

Bruns

et al. 2021

Preprint

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Understanding the biomechanics of tendon entheses is fundamental for surgical repair and tissue engineering, but also relevant in biomimetics and palaeontology. 3D imaging is becoming increasingly important in the examination of soft tissue deformation. But entheses are particularly difficult objects for micro-computed tomography because they exhibit extreme differences in X-ray attenuation. In this article, the ex vivo examination of Achilles tendon entheses from mice using a combination of tensile tests and synchrotron radiation-based micro-computed tomography is reported. Two groups of specimens with different water content are compared with regard to strains and volume changes in the more proximal free tendon and the distal tendon that wraps around the Tuber calcanei. Tomograms of relaxed and deformed entheses are recorded with propagation-based phase contrast. The tissue structure is rendered in sufficient detail to enable manual tracking of patterns along the tendon, as well as 3D optical flow analysis in a suitable pair of tomograms. High water content is found to increase strain and to change the strain distribution among proximal and distal tendon. In both groups, the volume changes are higher in the distal than in the proximal tendon. These results support the existence of a compliant zone near the insertion. They also show that the humidity of the specimen environment has to be controlled. Necessary steps to extend the automatic tracking of tissue displacements to all force steps are discussed.

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“…Current laryngeal image modalities include Laryngeal VS, Videokymography (VKG), High-Speed Digital Kymograghy (DKG) and Laryngeal HSV. However, there are new modalities that emerge for analyzing the vocal folds dynamics [22] and also devices for 3D visualization of the vibration of vocal folds superior margins [23][24][25][26]. This paper is focused on two laryngeal image modalities: VS and HSV.…”

Section: Laryngeal Image Modalitiesmentioning

confidence: 99%

Laryngeal Image Processing of Vocal Folds Motion

et al. 2020

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This review provides a comprehensive compilation, from a digital image processing point of view of the most important techniques currently developed to characterize and quantify the vibration behaviour of the vocal folds, along with a detailed description of the laryngeal image modalities currently used in the clinic. The review presents an overview of the most significant glottal-gap segmentation and facilitative playbacks techniques used in the literature for the mentioned purpose, and shows the drawbacks and challenges that still remain unsolved to develop robust vocal folds vibration function analysis tools based on digital image processing.

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3D multiscale imaging of human vocal folds using synchrotron X-ray microtomography in phase retrieval mode

Cited by 31 publications

References 66 publications

Quantitative evaluation of the human vocal fold extracellular matrix using multiphoton microscopy and optical coherence tomography

Quantitative evaluation of the human vocal fold extracellular matrix using multiphoton microscopy and optical coherence tomography

Gaining Insight into the Deformation of Achilles Tendon Entheses in Mice

Laryngeal Image Processing of Vocal Folds Motion

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