Ethanol fermentation from Jerusalem artichoke powder using Saccharomyces cerevisiae KCCM50549 without pretreatment for inulin hydrolysis

Invertase SUC2 Is the Key Hydrolase for Inulin Degradation in Saccharomyces cerevisiae

“…Invertase has been reported to have low activity with inulin, but the DP bound of the digestion was previously unknown (6). Generally, S. cerevisiae strains are only able to utilize fructooligosaccharides with a DP value up to around 6 (6,11). Our study demonstrated that SUC2 from strain JZ1C hydrolyzed fructooligosaccharides with a DP value of 20.…”

“…With the aid of genomic tools, a significantly wide range of genotype and phenotype variations has been realized for diversified yeast populations (7)(8)(9)(10). Recently, strains of S. cerevisiae have been reported to be able to utilize inulin and convert inulin-type sugars to ethanol (11). We also identified strains able to utilize inulin from varied S. cerevisiae populations (12).…”

<b>Agave syrup as a substrate for inulinase production by <i>Kluyveromyces marxianus</i> NRRL Y-7571

“…The application of inulinases in high-fructose syrup production has been investigated for decades (Vandamme & Derycke, 1983;Parekh & Margaritis, 1985;Etallibi & Baratti, 2001). In addition, these enzymes have many other applications, such as in the production of fructooligosaccharides, which are fermentable sugars that enhance alcoholic beverages production, such as tequila, and biofuels production from inulin-rich substrates (Chi et al, 2011;Lim et al, 2011;Waleckx et al, 2011;Flores-Gallegos, Morlett-Chávez, Aguilar, & Rodríguez-Herrera, 2012).…”

Yacon Flour and Corn Steep Liquor as Substrate for Inulinase and Biomass Production by Kluyveromyces Marxianus NRRL Y-7571

“…They have received a lot of attention recently due to their functional properties, because they improve food texture and fiber content without altering the appearance and flavor of the end product and they also have a beneficial effect on the intestinal flora and serum parameters due to their non-digestibility [1][2][3][4]. Furthermore, they have been used as a renewable raw material to produce several substances of industrial interest, especially high fructose (HFS) syrup and bioethanol [5][6][7][8]. Many plants store inulin as reserve carbohydrate, usually in bulbs, tubers or tuberous roots, such as Jerusalem artichoke (Helianthus tuberosus), dahlia (Dahlia pinnata), chicory (Cichoriumendivia), dandelion (Taraxacum officinale) and yacon (Smallanthus sonchifolius) [6,9].…”