Invertase SUC2 Is the Key Hydrolase for Inulin Degradation in Saccharomyces cerevisiae

Wang, Shian; Li, Fu‐Li

doi:10.1128/aem.02658-12

Cited by 36 publications

(32 citation statements)

References 24 publications

(23 reference statements)

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“…2). Our previous study observed that disruption of the SUC2 gene in the parental strain of JZH completely inhibited its growth in inulin but it remained slow growth on sucrose (Wang & Li, 2013). This finding indicated that there are additional enzymes possessing low invertase activities in the strain JZH, which results in sucrose utilization and benefits high-content FOS production by the strain.…”

Section: Resultsmentioning

confidence: 75%

“…The yeast S. cerevisiae can hydrolyze inulin with low DP into fructose and glucose by secretively expression of the invertase SUC2 that has an exo-inulinase activity (Wang & Li, 2013;Li, Fu et al, 2015). To remove the exo-inulinase activity, the SUC2 gene was disrupted in the S. cerevisiae strain JZH generating a strain JZHΔS.…”

Section: Resultsmentioning

confidence: 99%

“…(Wang et al, 2016) pUG6-TSC Ty1-P TEF1 -SP SUC2 -InuC opz -T CYC1 This study pUG6-ΔSUC2 DNA cassette for deletion of SUC2 (Wang & Li, 2013) …”

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confidence: 99%

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A one-step bioprocess for production of high-content fructo-oligosaccharides from inulin by yeast

Wang

2016

Carbohydrate Polymers

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Section: Resultsmentioning

confidence: 75%

Section: Resultsmentioning

confidence: 99%

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A one-step bioprocess for production of high-content fructo-oligosaccharides from inulin by yeast

Wang

2016

Carbohydrate Polymers

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“…5 that more monosaccharides (glucose and fructose) were released from inulin by the action of the recombinant inulinase produced by the transformant W14-3-INU-112 than by that of invertase produced by the strain W12d carrying the DNA fragments without the INU1 gene, indicating that the INU1 gene cloned in this study indeed was expressed in the transformant W14-3-INU-112 and inulin hydrolysis was enhanced by the action of the recombinant inulinase produced by the transformant W14-3-INU-112. Indeed, many results have shown that the invertase produced by various strains of S. cerevisiae have low inulinase activity [26,27]. That was why the inulinase gene obtained in this study was expressed in Saccharomyces sp.…”

Section: Discussionmentioning

confidence: 90%

Cloning and Characterization of an Inulinase Gene From the Marine Yeast Candida membranifaciens subsp. flavinogenie W14-3 and Its Expression in Saccharomyces sp. W0 for Ethanol Production

et al. 2014

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The INU1 gene encoding an exo-inulinase from the marine-derived yeast Candida membranifaciens subsp. flavinogenie W14-3 was cloned and characterized. It had an open reading frame of 1,536 bp long encoding an inulinase. The coding region of it was not interrupted by any intron. The cloned gene encoded 512 amino acid residues of a protein with a putative signal peptide of 23 amino acids and a calculated molecular mass of 57.8 kDa. The protein sequence deduced from the inulinase gene contained the inulinase consensus sequences (WMNDPNGL), (RDP), ECP FS and Q. The protein also had six conserved putative N-glycosylation sites. The deduced inulinase from the yeast strain W14-3 was found to be closely related to that from Candida kutaonensis sp. nov. KRF1, Kluyveromyces marxianus, and Cryptococcus aureus G7a. The inulinase gene with its signal peptide encoding sequence was subcloned into the pMIRSC11 expression vector and expressed in Saccharomyces sp. W0. The recombinant yeast strain W14-3-INU-112 obtained could produce 16.8 U/ml of inulinase activity and 12.5 % (v/v) ethanol from 250 g/l of inulin within 168 h. The monosaccharides were detected after the hydrolysis of inulin with the crude inulinase (the yeast culture). All the results indicated that the cloned gene and the recombinant yeast strain W14-3-INU-112 had potential applications in biotechnology.

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“…However, it has been well documented that K. marxianus cannot tolerate high concentrations of ethanol differently from Saccharomyces cerevisiae that is also a widely used industrial microorganism for its high ethanol production (Lane and Morrissey 2010;Nevoigt 2008;Ostergaard et al 2000). Unfortunately, S. cerevisiae cannot produce high concentrations of ethanol from inulin due to its weak capability of utilizing fructooligosaccharides with a high degree of polymerization Lim et al 2011;Wang and Li 2013). Therefore, it has been confirmed that introduction of a heterologous inulinase gene into S. cerevisiae could improve its inulin utilization and ethanol production.…”

Section: Introductionmentioning

confidence: 98%

Enhanced expression of the codon-optimized exo-inulinase gene from the yeast Meyerozyma guilliermondii in Saccharomyces sp. W0 and bioethanol production from inulin

Liu

Chi

2014

Appl Microbiol Biotechnol

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In the present study, after the exo-inulinase gene INU1 from Meyerozyma guilliermondii was optimized according to the codon usage bias of Saccharomyces cerevisiae, both the optimized gene INU1Y and the native gene INU1 were ligated into the homologous integration expression vector pMIRSC11 and expressed in Saccharomyces sp. W0. It was determined that the inulinase activity of the recombinant yeast Y13 with the optimized gene INU1Y was 43.84 U/mL, which was obviously higher than that (31.39 U/mL) produced by the recombinant yeast EX3 with the native gene INU1. Moreover, it was indicated that the recombinant yeast Y13 could produce 126.30 mg/mL ethanol from 300.0 g/L inulin while the recombinant yeast EX3 and Saccharomyces sp. W0 produced 122.75 mg/mL and 114.15 mg/mL ethanol, respectively, under the same conditions. In addition, the ethanol productivity of the recombinant yeast Y13 was 2.25 mg/mL/h within 48 h of the fermentation, which was obviously higher than that of the recombinant yeast EX3 (1.97 mg/mL/h) and Saccharomyces sp. W0 (1.77 mg/mL/h) within the same period. The results demonstrated that the recombinant yeast Y13 had higher ethanol production and productivity than the recombinant yeast EX3 and Saccharomyces sp. W0. Therefore, it was concluded that the codon optimization of the exo-inulinase gene from M. guilliermondii effectively enhanced inulinase activity and improved ethanol production from inulin by Saccharomyces sp. W0 carrying the optimized inulinase gene.

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Invertase SUC2 Is the Key Hydrolase for Inulin Degradation in Saccharomyces cerevisiae

Cited by 36 publications

References 24 publications

A one-step bioprocess for production of high-content fructo-oligosaccharides from inulin by yeast

A one-step bioprocess for production of high-content fructo-oligosaccharides from inulin by yeast

Cloning and Characterization of an Inulinase Gene From the Marine Yeast Candida membranifaciens subsp. flavinogenie W14-3 and Its Expression in Saccharomyces sp. W0 for Ethanol Production

Enhanced expression of the codon-optimized exo-inulinase gene from the yeast Meyerozyma guilliermondii in Saccharomyces sp. W0 and bioethanol production from inulin

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