Ethanol extract of Tephrosia bracteolata leaves and its fractions ameliorates alloxan-induced diabetes and its associated complications in Wistar rat model

Idakwoji, Precious A.; Ekpo, Daniel Emmanuel; Joshua, Parker Elijah; Njoku, O. U.; Nwodo, O. F. C.

doi:10.1007/s13410-020-00900-w

Cited by 4 publications

(4 citation statements)

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“…In our previous study, we reported the antidiabetic potentials of the ethanol extract of Tephrosia bracteolata leaves (Fig. 1) [27]. Based on the result of the penultimate studies, the ethyl acetate fraction (EAF) was subjected to further fractionation by using column chromatographic technique.…”

Section: Discussionmentioning

confidence: 99%

Antidiabetic Activity, Molecular Identification and In silico Docking of Compounds in Chromatographic Fractions of Tephrosia bracteolata Guill. & Perr. (Fabaceae) Leaves

Idakwoji

Oniemola

Onoyima

et al. 2023

Preprint

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Background Recent efforts for the complementary treatment of diabetes have focused on medicinal plants and their bioactive compounds. Tephrosia bracteolata is one of such plants used in the management of diabetes but its anti-diabetic principles are yet to be identified. This study was aimed at identifying the compounds responsible for the antidiabetic activity of the ethylacetate fraction of Tephrosia bracteolata leaves and subsequently, carryout an in silico molecular docking of these compounds against key targets in the pathophysiology of diabetes. Methods The ethylacetate fraction (EAF) of T. bracteolata leaves was fractionated using Silica gel column chromatography to yield 100 fractions. Pooling together of fractions with similar thin layer chromatographic (TLC) mobility profile afforded seven major fractions (SF1- SF7). Preliminary phytochemical studies were carried out on the fractions using standard methods. The antidiabetic activity of the fractions was subsequently evaluated (at a dose of 200 mg/kg) against alloxan- induced diabetes in adult mice. GC-MS analysis was carried out on the fraction with the highest activity. Subsequently, some of the identified active compounds were docked against key targets in the pathology of diabetes using Auto Dock tool. Results Preliminary phytochemical analysis revealed the presence of terpenoids, saponins, steroids, glycosides, flavonoids, tannins and alkaloids in varying proportions in the fractions. The sub-fractions produced varying degrees of significant (p < 0.05) decrease in FBS at 12h and 24h- post-treatment. GC-MS analysis of the most active fraction (SF5) revealed the presence of thirty- six compounds among which are some that have been reported to possess direct or indirect antidiabetic properties. These are Mome-inositol, 2-methoxy-4-vinylphenol, 1-D-thio-glucitol, 4-Piperidinone, Hexadecanoic acid, 9- octadecanoic acid, n- hexadecanoic acid and D- allose. Molecular docking studies (Auto Dock tool) between Mome inositol, 1-D-thio-glucitol and alpha-glucosidase showed that Mome inositol (− 6.7 kcal/mol) had a stronger affinity to the enzyme. Similarly, for sodium glucose co-transporter 2 (SGLT 2), Mome inositol (− 6.5 kcal/mol) had a stronger affinity than 1-D-thio-glucitol. Conclusions The identified compounds in the fraction could be responsible for the observed antidiabetic properties of the fraction of T. bracteolata.

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Section: Discussionmentioning

confidence: 99%

Antidiabetic Activity, Molecular Identification and In silico Docking of Compounds in Chromatographic Fractions of Tephrosia bracteolata Guill. & Perr. (Fabaceae) Leaves

Idakwoji

Oniemola

Onoyima

et al. 2023

Preprint

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“…intra-peritoneally. 12,13 Rats with fasting blood glucose (FBG) level greater than 200 mg/dl 72 h post induction were considered diabetic and included in the investigation.…”

Section: Induction Of Diabetes In Ratsmentioning

confidence: 99%

“…Polyphenols such as phenolics and flavonoids present in guava puree possess antioxidant properties and could be responsible for inhibiting the scavenging effect of free radicals produced by alloxan administration, the regeneration of the beta cells, release of insulin and consequently a decline in the blood glucose level. 13 Also, the guava purée could have facilitated the uptake of glucose by the peripheral cells, leading to a decline in the blood glucose level, 18 or promoting insulin secretion by closure of K+ ATP channels, membrane depolarization, and stimulation of calcium influx into the cells, which is an important step in secretion of insulin. 19 This finding is in consonance with the report of 20,10 .…”

Section: Effect Of White Psidium Guajava Purée Treatment On Random Bl...mentioning

confidence: 99%

Antidiabetic and Hepatoprotective Effects of Psidium guajava L Fruit Puree on Alloxan-induced Diabetes in Wistar Rats

2022

TJNPR

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Psidium guajava L. (fam. Myrtaceae) is a semi-deciduous tree endemic to tropical and subtropical areas. The leaves, roots and bark have long been used for folkloric and medicinal purposes such as in the treatment of hypertension, fever, toothache and diarrhoea. The purpose of this study was to evaluate the antidiabetic and hepatoprotective effects of Psidium guajava fruits in alloxan-induced diabetic rats. Fresh guava fruits were chopped and blended into a smooth paste (purée). The purée was weighed, dissolved in freshly prepared normal saline in appropriate stock concentrations and used in a 21-day treatment. Acute toxicity studies of the fruit purée conducted in mice showed no toxicity up to 5000 mg/kg. Experimental animals were acclimatized for 7 days while diabetes was induced using alloxan monohydrate (150 mg/kg) intra-peritoneally. After a 21-day treatment of diabetic rats with 200 and 400 mg/kg of Psidium guajava fruit purée, the fasting and random blood glucose levels decreased significantly (p < 0.05) relative to that of the diabetic control. Also, there was a significant reduction in serum aspartate aminotransferase, alanine aminotransferase and alkaline phosphatase levels of rats treated with 200 and 400 mg/kg of Psidium guajava fruit purée compared to the diabetic control. Thus, it could be inferred that Psidium guajava fruit purée is safe at high doses, suppresses hyperglycemia and protects the liver by decreasing lipid peroxidation and improve antioxidant status in alloxan-induced diabetic rats. These results provide the scientific evidence for its use as a nutraceutical agent in the management of diabetes and hepatotoxicity.

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“…Medicinal plants possess numerous phytocompounds that might lead to the discovery of new drugs that may be used to prevent and manage diseases (Lahlou, 2013;Idakwoji et al, 2021a;2021b). Anogeissus leiocarpa (DC.)…”

Section: Introductionmentioning

confidence: 99%

Pharmacological studies of anti-inflammatory, anti-nociceptive and anti-pyretic compounds found in chromatographic fractions of Anogeissus leiocarpa (DC). Guill. & Perr. leaves

Idakwoji¹,

Atanu²,

Nweje-Anyalowu³

et al. 2022

J Pharm Pharmacogn Res

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Context: The anti-nociceptive and anti-pyretic properties of the crude aqueous extract of Anogeissus leiocarpa (DC). Guill. & Perr. leaves have been reported. Aims: To investigate the anti-inflammatory, anti-nociceptive, and anti-pyretic properties of aqueous extract of A. leiocarpa leaves (AEAL) and its fractions to identify the bioactive compound(s). Methods: AEAL was fractionated successively and eluted in gradients of solvent mixture (n-hexane, ethylacetate, and methanol). Eluates, which showed similar TLC profiles were pooled to afford 5 fractions (F1-F5). AEAL and the fractions were subjected to phytochemical analysis and investigated for anti-inflammatory, anti-nociceptive, and anti-pyretic effects using carrageenan-induced paw edema and Brewer’s yeast-induced pyrexia in rats, and acetic acid-induced writhing in mouse models, respectively. Results: Phytochemical analysis indicated presence of terpenoids, saponins, steroids, glycosides, flavonoids, tannins and alkaloids. At 200 mg/kg b.w, AEAL and F1-F5 recorded significant (p<0.05) improvements in paw edema, abdominal constrictions and pyrexia, which is comparable to the standard (ketoprofen or Aspirin), relative to the control groups, with F4 observed to be the most potent fraction. GC-MS analysis of F4 reveal the presence of hexadecenoic acid, 15-methyl-, methylester, 9-oxabicyclo (6.1.0) nonane, cis-, cholest-4-en-3-one, cholesterol and 3-diazo-1-methyl-1, 3-dihydro-indol-2-one. Conclusions: The results indicate that identified compounds, particularly 3-dihydro-indol-2-one, could be responsible for the anti-inflammatory, anti-nociceptive, and anti-pyretic properties of the extract.

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Ethanol extract of Tephrosia bracteolata leaves and its fractions ameliorates alloxan-induced diabetes and its associated complications in Wistar rat model

Cited by 4 publications

References 16 publications

Antidiabetic Activity, Molecular Identification and In silico Docking of Compounds in Chromatographic Fractions of Tephrosia bracteolata Guill. & Perr. (Fabaceae) Leaves

Antidiabetic Activity, Molecular Identification and In silico Docking of Compounds in Chromatographic Fractions of Tephrosia bracteolata Guill. & Perr. (Fabaceae) Leaves

Antidiabetic and Hepatoprotective Effects of Psidium guajava L Fruit Puree on Alloxan-induced Diabetes in Wistar Rats

Pharmacological studies of anti-inflammatory, anti-nociceptive and anti-pyretic compounds found in chromatographic fractions of Anogeissus leiocarpa (DC). Guill. & Perr. leaves

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Ethanol extract of Tephrosia bracteolata leaves and its fractions ameliorates alloxan-induced diabetes and its associated complications in Wistar rat model

Cited by 4 publications

References 16 publications

Antidiabetic Activity, Molecular Identification and In silico Docking of Compounds in Chromatographic Fractions of Tephrosia bracteolata Guill. &amp; Perr. (Fabaceae) Leaves

Antidiabetic Activity, Molecular Identification and In silico Docking of Compounds in Chromatographic Fractions of Tephrosia bracteolata Guill. &amp; Perr. (Fabaceae) Leaves

Antidiabetic and Hepatoprotective Effects of Psidium guajava L Fruit Puree on Alloxan-induced Diabetes in Wistar Rats

Pharmacological studies of anti-inflammatory, anti-nociceptive and anti-pyretic compounds found in chromatographic fractions of Anogeissus leiocarpa (DC). Guill. & Perr. leaves

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Antidiabetic Activity, Molecular Identification and In silico Docking of Compounds in Chromatographic Fractions of Tephrosia bracteolata Guill. & Perr. (Fabaceae) Leaves

Antidiabetic Activity, Molecular Identification and In silico Docking of Compounds in Chromatographic Fractions of Tephrosia bracteolata Guill. & Perr. (Fabaceae) Leaves