Ethanol extract of Plantago asiatica L. controls intracellular fat accumulation and lipid metabolism in 3T3-L1 Adipocytes

Abstract

Objectives : The effects of ethanol extract of Plantago asiatica L. were investgated on adipocyte differentiation, lipopogenesis, lipolysis and apoptosis using differnentiated 3T3-L1 adipocytes.Methods : Plantago asiatica L. was extracted with ethanol (CCE). We carried on MTT assay for cell proliferation, Oil Red O staining for determination of cell differentiation and intracelluar adipogenesis. TUNEL staining assay for cell apoptosis, and Western blot analysis for measurement of pAMPK and pACC, C/EBPα , PPARγ protein expressions were performed. Results : The addition of CCE up to 0.2 mg/ml into cell culture media showed no cytotoxicity. Treatment of 0.2 mg/ml CCE significantly inhibited differentiation in 3T3-L1 preadipocytes. Lipid accumulation of the CCE treated cells was decreased compared with that of control. Induction of cell apoptosis was increased in CCE treated cells compared with that of control. AMPK and ACC levels of the cells with 0.2 mg/ml CCE were led to phosphorylation and also expressions of C/EBPα and PPARγ , as adipogenic transcription factors, were suppressed compared with those of control. Conclusions : Taken together, these results provide evidence that CCE has a regulatory role in lipid metabolism that is related to differentiation into adipocytes, adipogenesis and apoptosis.

Inhibition Effect of Adipogenesis and Lipogenesis via Activation of AMPK in Preadipocytes Treated with Canavalia gladiata Extract

“…The CGE did not inhibit the growth of preadipocytes at ≤1.0 mg/mL, indicating that these concentrations did not inhibit cell viability. The above percentages were 4.64 times higher than that obtained in a previous study by Jeon et al, where a treatment with Plantago asiatica L. extracts at 1.0 mg/mL resulted in a cell viability of 20.0%, proving that the CGE is less cytotoxic to preadipocytes at this concentration compared to other existing natural products [14]. Therefore, 1.0 mg/mL was set as the maximum CGE concentration that would not inhibit preadipocyte growth during the subsequent experiments on the inhibition of preadipocyte differentiation and gene expression.…”

“…where a treatment with Plantago asiatica L. extracts at 1.0 mg/mL resulted in a cell viability of 20.0%, proving that the CGE is less cytotoxic to preadipocytes at this concentration compared to other existing natural products [14]. Therefore, 1.0 mg/mL was set as the maximum CGE concentration that would not inhibit preadipocyte growth during the subsequent experiments on the inhibition of preadipocyte differentiation and gene expression.…”

Effect of Centella asiatica Extract on Anti-obesity Suppression via Inhibition of Adipogenesis-related Gene Expression in Preadipocyte

“…A previous study by Jeon et al, where adipocytes were treated with 1.0 mg/mL of Plantago asiatica extract, reported a cell survival rate of 18.4%, which is lower than that obtained in the present study. Moreover, the extract was considered as a highly safe natural material due to its higher non-toxicity (Jeon et al, 2014).…”