Ethanol Enhances the Stimulatory Effects of Insulin and Insulin-like Growth Factor-I on DNA Synthesis in NIH 3T3 Fibroblasts

Tomono, Masahiro; Kiss, Zoltán

doi:10.1006/bbrc.1995.1305

Cited by 26 publications

(12 citation statements)

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“…The eŠect of two compounds in the mixture was judged to be synergistic when the FIC value was smaller than 0.9 and additive when it was 0.9 to 1.1, according to the method of Mucsi et al 12) Incorporation of radioactive precursors into bacterial macromolecules. To elucidate whether ‰avo-noids could inhibit the synthesis of DNA in the test bacteria, the incorporation of radioactive thymidine into bacterial cells was studied according to the method of Tomono et al 21) An aliquots (0.1 ml) of the culture that had been incubated overnight at 379 C was added to 0.4 ml of Mueller-Hinton broth containing 1 mM [methyl-3 H]thymidine (ˆnal concentration of 1 mCi) and a ‰avonoid (0 to 50 mg). After incubating at 379 C for 2 h, the culture was centrifuged at 10,000 rpm for 10 min, and the supernatant was decanted.…”

Section: Methodsmentioning

confidence: 99%

Rutin-enhanced Antibacterial Activities of Flavonoids againstBacillus cereusandSalmonella enteritidis

Arima¹,

Ashida²,

Danno³

2002

Bioscience, Biotechnology, and Biochemistry

202

119

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The antibacterial activities of flavonoids were found by the paper disk method to be enhanced by combining or mixing them. The combinations of quercetin and quercitrin, quercetin and morin, and quercetin and rutin were much more active than either flavonoid alone. Although rutin did not show activity in itself, the antibacterial activities of quercetin and morin were enhanced in the presence of rutin. The antibacterial activities of flavonoids, in combination with morin and rutin, were evaluated, based on the minimum inhibition concentration (MIC) in a liquid culture, by using Salmonella enteritidis and Bacillus cereus as the test bacteria. The activities of galangin, kaempherol, myricetin and fisetin were each enhanced in the presence of rutin when S. enteritidis was used as the test bacterium. The MIC value for kaempherol was markedly decreased by the addition of rutin. Morin inhibited DNA synthesis, and this effect was promoted by rutin at a concentration of 25 microg/ml.

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Section: Methodsmentioning

confidence: 99%

Rutin-enhanced Antibacterial Activities of Flavonoids againstBacillus cereusandSalmonella enteritidis

Arima¹,

Ashida²,

Danno³

2002

Bioscience, Biotechnology, and Biochemistry

202

119

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“…Like bovine spleen NMT [43], human NMT was also found to be activated several folds with organic solvents such as ethanol and acetonitrile [43]. It has been reported that ethanol enhances the stimulatory effect of insulin and insulin-like growth factor-1 on DNA synthesis in NIH 3T3 fibroblasts [44]. It has also demonstrated that ethanol enhances the expression of cell surface class I major histocompatibility complex antigens in a variety of cell lines [45].…”

Section: Resultsmentioning

confidence: 99%

Biochemical Characterization of Bovine Brain Myristoyl‐CoA:Protein N‐Myristoyltransferase Type 2

Selvakumar

Lakshmikuttyamma

Sharma

2009

BioMed Research International

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Protein N-myristoylation is a lipidic modification which refers to the covalent attachment of myristate, a 14-carbon saturated fatty acid, to the N-terminal glycine residue of a number of mammalian, viral, and fungal proteins. In this paper, we have cloned the gene coding for myristoyl-CoA:protein N-myristoyltransferase (NMT) from Bos tarus brain. The open reading frame codes for a 410-amino-acid protein and overexpressed in Escherichia coli. Kinetic studies suggested that bovine brain NMT2 and human NMT1 show significant differences in their peptide substrate specificities. The metal ion Ca2+ had stimulatory effects on NMT2 activity while Mn2+ and Zn2+ inhibited the enzyme activity. In addition, NMT2 activity was inhibited by various organic solvents and other detergents while NMT1 had a stimulatory effect. Biochemical characterization suggested that both forms of NMT have unique characteristics. Further analysis towards functional role NMT2 will lead the development of therapeutic target for the progression of various diseases such as cancer, cardiovascular diseases, and neurodegenerative diseases.

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“…Maximum stimulatory effects were visible at 0.01% and at higher concentrations the stimulatory effects decreased. Stimulatory effects were also visible for fibroblasts (e.g., Tomono, 1995;Kiss et al, 1998). In a few studies, a biphasic dose-depending ethanol effect was described.…”

Section: Discussionmentioning

confidence: 97%

Effects of Ethanol Treatment on Proliferation and Differentiation in a Head and Neck Squamous Cell Carcinoma Cell Line

Kornfehl

Temmel

Formanek

et al. 1999

Alcoholism Clin & Exp Res

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Ethanol Enhances the Stimulatory Effects of Insulin and Insulin-like Growth Factor-I on DNA Synthesis in NIH 3T3 Fibroblasts

Cited by 26 publications

References 0 publications

Rutin-enhanced Antibacterial Activities of Flavonoids againstBacillus cereusandSalmonella enteritidis

Rutin-enhanced Antibacterial Activities of Flavonoids againstBacillus cereusandSalmonella enteritidis

Biochemical Characterization of Bovine Brain Myristoyl‐CoA:Protein N‐Myristoyltransferase Type 2

Effects of Ethanol Treatment on Proliferation and Differentiation in a Head and Neck Squamous Cell Carcinoma Cell Line

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