Ethanol enhanced in vivo gene delivery with non-ionic polymeric micelles inhalation

Bioavailability Effect of Methylprednisolone by Polymeric Micelles

“…The formation of PEO-PPO-PEO PM was confirmed by a fluorescence probe technique using pyrene; the partition of pyrene into the micellar phase was determined using the ratio of peak I 1 /peak I 3 of the pyrene spectrum as previously reported (25,31,32). The fluorescence emission spectra of pyrene in the PEO-PPO-PEO polymeric micelles solutions were measured from 350 to 500 nm using a fixed excitation wavelength of 339 nm with a constant pyrene concentration of 6 Â 10 j7 M. The concentration of PEO-PPO-PEO polymers used varied from 1 Â 10 j4 to 10% (w/w).…”

“…The average particle size and Zeta potential of MP/PM were determined by quasielastic laser dynamic light scattering (DLS) (Zetasizer 3000; Malvern Instruments, Malvern, UK), using an assumed refractive index ratio of 1.33 and a viscosity of 0.88 (31,32). The sampling time for each sample was 10 ms and the experimental duration was 100 s. All measurements were performed at 25-C at a measurement angle of 90-and results were presented as mean T S.E.M.…”

“…The AFM (CP-II; Digital Instruments/Veeco Metrology Group, Santa Barbara, CA) was operated in constant tapping mode, as described in the previous study (31). The cantilevers were standard NanoProbe silicon single-rectangular cantilevers (NSC15/AIBS 230 mm) (MikroMasch, Estonia); the constant force mode was used with a typical scan frequency of 328 kHz.…”

Phosphatidylcholine embedded micellar systems: Enhanced permeability through rat skin

“…8). This result has generally been expected, since the incorporation of a lipophilic phase, especially ethanol, enhances drug permeability through the skin [42]. A comparison between O/W-like formulation without ethanol and a W/O-like formulation containing MCT and ethanol ( Fig.…”

Tissue-Clearing Techniques Enable Three-Dimensional Visualization of Aerosolized Model Compound and Lung Structure at the Alveolar Scale

“…Various drug carriers for the pulmonary delivery to the focus site have been investigated. [4][5][6][7] As part of the development processes of these systems, drug delivery efficiency in the lungs must be evaluated via visualization at the alveolar scale. Although X-ray computed tomography (CT), positron emission tomography (PET), and single-photon emission computed tomography (SPECT) enable the three-dimensional (3D) visualization of lung structure and the distribution of contrast agents administrated intrapulmonarily on a macroscopic tissue scale, 8) visualization at the alveolar scale for the evaluation of drug distribution at focus sites is impossible with present technologies owing to their low resolution.…”