Ethanol Concentrations in Antemortem Blood Samples Under Controlled Conditions

Petković, Stojan; Savić, Slobodan; Zgonjanin, Dragana; Samojlik, Isidora

doi:10.1093/alcalc/agn072

Cited by 18 publications

(12 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In one study loss of ethanol from opened tubes (mean 0.015 g/L) was greater than from unopened tubes (mean 0.010 g/L), after storage in a refrigerator for 13–39 months [17] . All bloods that were initially negative for ethanol content remained negative after short-term [22] and long-term storage under various conditions of time and temperature [15] , [17] .…”

Section: Discussionmentioning

confidence: 99%

Decreases in blood ethanol concentrations during storage at 4 °C for 12 months were the same for specimens kept in glass or plastic tubes

Jones

Ericsson

2016

Practical Laboratory Medicine

View full text Add to dashboard Cite

BackgroundThe stability of ethanol was investigated in blood specimens in glass or plastic evacuated tubes after storage in a refrigerator at 4 °C for up to 12 months.MethodsSterile blood, from a local hospital, was divided into 50 mL portions and spiked with aqueous ethanol (10% w/v) to give target concentrations of 0.20, 1.00, 2.00 and 3.00 g/L. Ethanol was determined in blood by headspace gas chromatography (HS-GC) with an analytical imprecision of <3% (coefficient of variation, CV%). Aliquots of blood were re-analysed after 2, 7, 14, 28, 91, 182 and 364 days of storage at 4 °C.ResultsThe standard deviation (SD) of analysis by HS-GC was 0.0059 g/L at 0.20 g/L and 0.0342 g/L at 3.00 g/L, corresponding to CVs of 2.9% and 1.1%, respectively. The decreases in blood ethanol content were analytically significant after 14–28 days of storage for both glass and plastic tubes The mean (lowest and highest) loss of ethanol after 12 months storage was 0.111 g/L (0.084–0.129 g/L) for glass tubes and 0.112 g/L (0.088–0.140 g/L) for plastic tubes. The corresponding percentage losses of ethanol were 43–45% at a starting concentration of 0.20 g/L and 3.9–4.1% at 3.00 g/L.ConclusionThe concentration of ethanol in blood gradually decreases during storage at 4 °C. After 12 months storage the absolute decrease in concentration was ~0.11 g/L when the starting concentration ranged from 0.20 to 3.0 g/L. Decreases in ethanol content were the same for specimens kept in glass or plastic evacuated tubes.

show abstract

Section: Discussionmentioning

confidence: 99%

Decreases in blood ethanol concentrations during storage at 4 °C for 12 months were the same for specimens kept in glass or plastic tubes

Jones

Ericsson

2016

Practical Laboratory Medicine

View full text Add to dashboard Cite

show abstract

“…Results of many studies have shown that microorganisms as found in our tested samples can cause glucose to ethanol conversion. 4,7,12–14 So it is important to recognize that ethanol is formed as a result of the microorganisms’ action, not drinking an alcoholic beverage. Also, it is important to correctly interpret the urine ethanol concentration, especially if the blood sample was not taken.…”

Section: Resultsmentioning

confidence: 99%

“…The amount of generated ethanol depends on the species of microorganisms present, the available substrates, the temperature and time of storage, and the presence of preservatives added to the specimens. 3,4…”

Section: Introductionmentioning

confidence: 99%

Microbial ethanol production in postmortem urine sample

et al. 2013

View full text Add to dashboard Cite

We present a case in which postmortem blood ethanol concentration was 0.02 g/kg and acetone concentration was 0.51 g/kg, while urine ethanol concentration was 6.0 g/kg and acetone concentration was 0.63 g/kg. In the urine sample, sodium fluoride was not added. The urinary ethanol concentration continued to increase without any remarkable increase of isopropanol concentration and external contamination was excluded. Species of bacteria and yeasts, including Candida glabrata, were isolated from urine and blood samples. A few days after the collection of samples, we received the information that the patient was diabetic and did not receive insulin therapy regularly. To prevent postmortem microbial ethanol production and incorrect diagnosis of the cause of death, it is necessary to add sodium fluoride to blood and urine samples collected from diabetic patients.

show abstract

“…Kanitin kommnasini saglamak için hasta giysiletini çikanrken odadan çikilmamahdir. Klinik alanlarda, kilidi hulunan camli ve rafli bir dolapta kanitlann teslim edilene kadar sakli tutulmasi kanitlann korunmasinda son derece önemlidir [38,39]. Kiyafetler olabildigince az katlanmah, sallanmamah ve yen degiçtirilmemelidir.…”

Section: C Elbiseler Ve Di § Ortaindan Biyolojik Numune Toplanmasiunclassified

Collection And Transfer Of Biological Materials From Forensic Cases In Emergency Units

Karadayı¹,

Kolusayin²,

Kaya³

et al. 2013

MMJ

View full text Add to dashboard Cite

Ethanol Concentrations in Antemortem Blood Samples Under Controlled Conditions

Abstract: Surprisingly, this study showed no ethanol production in improperly processed antemortem blood samples in healthy and sober individuals who were subjected to overnight fasting.

Cited by 18 publications

References 7 publications

Decreases in blood ethanol concentrations during storage at 4 °C for 12 months were the same for specimens kept in glass or plastic tubes

Decreases in blood ethanol concentrations during storage at 4 °C for 12 months were the same for specimens kept in glass or plastic tubes

Microbial ethanol production in postmortem urine sample

Collection And Transfer Of Biological Materials From Forensic Cases In Emergency Units

Contact Info

Product

Resources

About