In this paper the effect of extractant on the biochemical composition of Brazilian Cerrado biome plant species (Sapindus saponaria L., Guazuma ulmifolia Lamb., Eugenia dysenterica Mart. and Stryphnodendron adstringens Mart.) was presented. About 50% of S. saponaria, G. ulmifolia, E. dysenterica and S. adstringens were mainly consisted of lipids. There was a great variety of protein content among the tested species and presence of peroxidase activity (903.54 EU/g). About the antinutritional factors, considerable quantity of lectins (10,752 UH/g) and trypsin inhibitor was also detected (139 IU/g). These plants were considered as source of biomolecules that can largely be explored for biotechnological applications.