Estudo comparativo de éguas repetidoras ou não de cio através da avaliação histológica do endométrio e das concentrações plasmáticas de progesterona

Abstract

Comparative study of repeat breeds and healthy mares through endometrial Comparative study of repeat breeds and healthy mares through endometrial Comparative study of repeat breeds and healthy mares through endometrial Comparative study of repeat breeds and healthy mares through endometrial Comparative study of repeat breeds and healthy mares through endometrial histology and plasmatic progesterone concentrations histology and plasmatic progesterone concentrations histology and plasmatic progesterone concentrations histology and plasmatic progesterone concentrations histology and plasmatic progesterone concentrations.] Estudo comparativo de éguas repetidoras ou não de cio através da avaliação histológica do endométrio e das concentrações plasmáticas de progesterona. Pesquisa Veterinária Brasileira 27 (12) The study aimed to compare endometrial histology and plasmatic progesterone (P4) concentration of repeat breeds and healthy mares. The hypothesis was that there is a correlation between infertility and endometrial histology and P4 concentration in both groups. A total of 36 Campolina and Mangalarga Marchador mares in reproductive age (3-23 years) were used, 11 of them were healthy mares (Control group, 7 embryo recipient and 4 embryo donors), and 25 repeat breeders (10 embryo recipient and 15 embryo donors), classified as based on their reproductive history. Endometrial and blood samples were collected for respectively histological and plasma progesterone concentration evaluation. The endometrial samples obtained after biopsy were fixed in Bouin's fluid, processed, included in paraffin, and stained with HematoxylinEosin (HE) for histopathological examination. Plasmatic progesterone concentrations were evaluated by enzyme immunoessay (ELISA). There was no correlation between progesterone concentration and fertility. But there was a positive correlation between age and fertility, as older mares had major tendency of subfertility than younger ones. There was also a correlation between biopsy categories and fertility, as more histological alterations were found, higher were the chances for the mares to be subfertile. However not all mares classified as Category I and II maintained pregnancy until parturition. Other factors could influence pregnancy maintenance. In the same way, not all mares in Category III were infertile. The endometrial biopsy was shown to be an easy and cheap diagnostic technique with minimal discomfort to the animals and, together with other data, to be a very important component in the investigation of mare fertility.

Morphological and morphometric evaluation of prepubertal gilt ovaries, uterine tubes and uterus at different oestrus cycle stages

“…Following, the material obtained was fixed in Bouin solution (75 % saturated picric acid solution, 25% formaldehyde and 5% glacial acetic acid) for a period of 24 hours (Eigenheer- Moreira et al 2007, Moraes et al 2007, Aleixo et al 2011. Then, the samples were dehydrated with increasing concentrations of ethanol, cleared in xylene and included in paraffin (Drummond et al 2004).…”

The gynecological examination in Pantaneiro mares

“…Moreover, not all mares with more severe changes present reproductive failure (Silva et al, 1987). However, subfertility was directly related to the animal's age (Eigenheer-Moreira et al, 2007).…”

“…The only mare that had an endometrium close to normal was mare 6, in which no change of any kind was observed regarding inflammatory infiltrates and fibrosis, and was therefore classified into category I. A study by Eigenheer-Moreira et al (2007) concluded that the animals that presented the most severe endometritis and fibrosis as histological changes were those with more estrus repetitions. The presence of mild to moderate changes does not necessarily imply that the mare will not be able to carry a pregnancy to term.…”

The association between the endometrial biopsy grade and selected epidemiological and reproductive variables in a population of subfertile mares

“…In the hands of a reasonably experienced operator, this procedure has few complications and side effects (Van Camp, 1988), and there is no apparent negative effects on reproductive performance (Kenney, 1978). Endometrial biopsy is an established minor surgical procedure that is modestly invasive, easy and a cheap diagnostic technique, with the most common sequelae being slight bleeding at the biopsy site (Eigenheer‐Moreira et al, 2007; Sertich, 1996; Snider et al, 2011). This rapid technique is frequently included in the examination for breeding soundness evaluation and additionally is capable of estimating the mare's ability to carry a foal to term (Snider et al, 2011).…”