This study describes extracellular biosynthesis of cadmium sulfide quantum dots by Fusarium oxysporum f. sp. lycopersici. Mycelia was incubated with a cadmium sulfate solution at 30°C and after 12 days the mixture became yellow, then the biomass was discarded through paper filtration. The filtrate containing extracellular cadmium sulfide quantum dots displayed increased UV-Vis absorption from 300 - 500 nm and fluorescence at 520 nm which was not shown when incubated without cadmium sulfide, thus indicating the presence of biologically synthesized quantum dots. Transmission electron microscope analysis of biologically synthesized quantum dots evinced individual 2 - 6 nm diameter circular nanoparticles of uniform size. Energy dispersive spectroscopy confirmed the presence of S and Cd. Additionally, this study showed the relevance in the use of positive and negative controls when evaluating the biosynthesis of CdS quantum dots using UV-Vis and fluorescence spectrophotometry.