Estrogenicity of the isoflavone genistein pigeon pie seeds (<i>Cajanus cajan</i>L. Mill sp.) on reproductive organs in rat

Primiani, Cicilia Novi; Pujiati, Pujiati; Hardani,

doi:10.1088/1742-6596/1025/1/012061

Cited by 3 publications

(3 citation statements)

References 31 publications

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“…Tumbuhan memiliki peranan penting dalam kehidupan manusia, selain sebagai sumber bahan makanan, sandang, bahan bakar juga bahan-bahan industri 1 . Berbagai bahan kimia yang terdapat dalam tumbuhan juga dapat dimanfaatkan untuk obat-obatan, insektisida, dan kosmetika 2 . Bahan-bahan kimia tersebut bagi tumbuhan itu sendiri berfungsi sebagai media interaksi antara sesama tumbuhan maupun dengan makhluk hidup lain di sekitarnya serta untuk mempertahankan diri dari berbagai pengaruh luar 1 .…”

Section: Pendahuluanunclassified

Uji Skrining Fitokimia Ekstrak Etanol Daun Ubi Jalar Ungu (Ipomoea Batatas L)

Susanto

Hardani

Rahmawati

2019

arteri

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Purple sweet potato is a very familiar plant for us, and the most common is white sweet potato, purple, yellow ororange. The advantages of colored purple sweet potatoes contain anthocyanins. Anthocyanins are a secondarymetabolite of flavonoids and polyphenols that can act as antioxidants. This concentration of anthocyanins is whatcauses some kind of purple potato to have a gradient of different shades of purple. Anthocyanins provide excellenthealth effects namely as antioxidants and anticancer due to electron deficiency in its chemical structure so that it isreactive to resist free radicals. Sweet potato leaves can also be utilized as food ingredients as well as potentiallymedicines for various diseases. Purple sweet Potato (Ipomoea batatas L) is one of the agricultural commodities inIndonesia that has a number of production is quite abundant and can be used as a traditional medicine. Theproblem formulation in this study is whether purple sweet potato leaf extract (Ipomoea batatas L.) contains thechemical compounds of alkaloids, falovonoids, tannins and saponins.The purpose of this research is to know thecontent of chemical compounds in purple sweet potato leaf (Ipomoea batatas L). This research is an experimentalstudy that is by observing and conducting experimental observations of the group in various treatment conditions.The sample used in this study is the purple sweet potato leaf (Ipomoea batatas L). The results of this study showedthat the purple sweet potato leaves were positively contained.

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Section: Pendahuluanunclassified

Uji Skrining Fitokimia Ekstrak Etanol Daun Ubi Jalar Ungu (Ipomoea Batatas L)

Susanto

Hardani

Rahmawati

2019

arteri

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“…No literature was found to report the detection of these iso avones from the seeds of the C. cajan using chromatographic techniques, particularly HPLC. Genistein extracted from C. cajan seeds were found to have estrogenic activity on rat reproductive organs [27] and antibacterial activity when tested against Staphylococcus aureus strains [28]. In our study, methanolic seed extracts of C cajan were analysed with the optimized SPE-HPLC procedure.…”

Section: Discussionmentioning

confidence: 99%

High-Performance Liquid Chromatography (HPLC) Method Validation for Simultaneous Quantitation of Five Phytoestrogenic Flavonoids

Cruz

Quiming

Nicolas

et al. 2023

Preprint

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A Reversed Phase High Performance Liquid Chromatography (RP-HPLC) method for simultaneous quantitation of four isoflavone standards daidzein, genistein, formononetin and biochanin A, and one flavone standard quercetin was developed and validated through the evaluation of linearity, accuracy, precision, specificity, limit of detection and limit of quantitation in accordance with the ICH guidelines. The analysis was performed in a C18 column (150 x 4.6mm, 5µm) with an optimised gradient elution using acetonitrile-water (0.1% trifluoroacetic acid) at a flow rate of 1.0mL min-1 and sample injection volume of 10uL.The retention times of the standards in the order daidzein, quercetin, genistein, formononetin and biochanin A were 4.42, 5.24, 7.85, 10.06 and 13.55 minutes, respectively with tailing factors ranging from 1.09 – 1.12 and minimum resolution value of 3.74. Detection limits range from 0.339 to 0.964 ug/mL and quantitation limits range from 1.027 to 2.922 ug/mL with good linearity (R2 ≥ 0.9967) with a linear range of 1.25 - 20ug/mL for all standards. The method was also found to be accurate and precise based on percentage recovery ranging from 96.96% to 106.87% (intraday, n=3) and relative standard deviation of %RSD≤1.45% (intra-day, n=3) and %RSD≤2.35% (inter-day, n=5). The specificity of the method was evaluated based on the positivity of the minimum peak purity index during the quantitation of the target compounds from the spiked hydrolysed and unhydrolyzed extract of Cajanus cajan ICPL 7035.

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“…Lacy 23 reported that the structure of genistein and estrogen is almost similar and has an estrogenic effect that can bind to estrogen receptors (ER). According to Primiani et al 24 genistein is an isoflavone derivative and is the most estrogenic phytoestrogen while the isoflavone derivative content of combined A. sativum, C. mangga and A. calamus is not elucidated yet and still needs further research. E: endometrium, M: myometrium, P: perimetrium C-(no treatment), C + (clomiphene citrate 0.9 mg/kg BW), composition 1: T1 (50 mg/kg BW), T2 (75 mg /kg BW), T3 (100 mg/kg BW), composition 2: T4 (50 mg/ kg BW), T5 (75 mg/kg BW), T6 (100 mg/kg BW), T7 (Subur kandungan herb™ of 75 mg/kg BW).…”

Section: Level Of Estrogenmentioning

confidence: 99%

Efficacy of Allium sativum, Curcuma mangga and Acorus calamus Extract Combination on Rat Fertility

Muchtaromah¹,

Mutiah²,

Yusmalasari³

et al. 2020

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Objective: to evaluate the efficacy of Allium sativum, Curcuma mangga, and Acorus calamus extract combination on female rat fertility. Methods: Thirty-six female rats were randomized divided into 9 groups (n=4): C-(no treatment); C+ (clomiphene citrate); composition 1 (A. sativum 36%: C. mangga 36%: A. calamus 28%) with 3 doses i.e T1, T2 & T3 of 50, 75 & 100 mg/kg BW; composition 2 (A. sativum 35%: C. mangga 40%: A. calamus 25%) with 3 doses i.e T4, T5 & T6 of 50, 75 & 100 mg/kg BW and T7 (Subur Kandungan herb™). Treatment was given every day for 15 days. Results: The results revealed that all treatments affected reproductive hormone and uterine histology profile. However, T1 produced the highest estrogen and progesterone levels, and T2 produced the highest endometrial and myometrium thickness, as well as the number of endometrial glands. Furthermore, the hormonal profile in T1 and histological profile of uterus in T2 were not significantly different from those of T7 group. Conclusions: Combined A. sativum, C. mangga, and A. calamus extracts could be used to increase fertility in female rat. The most effective treatment in this study was dose of 50-75 mg/BW in both compositions.

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Estrogenicity of the isoflavone genistein pigeon pie seeds (Cajanus cajanL. Mill sp.) on reproductive organs in rat

Cited by 3 publications

References 31 publications

Uji Skrining Fitokimia Ekstrak Etanol Daun Ubi Jalar Ungu (Ipomoea Batatas L)

Uji Skrining Fitokimia Ekstrak Etanol Daun Ubi Jalar Ungu (Ipomoea Batatas L)

High-Performance Liquid Chromatography (HPLC) Method Validation for Simultaneous Quantitation of Five Phytoestrogenic Flavonoids

Efficacy of Allium sativum, Curcuma mangga and Acorus calamus Extract Combination on Rat Fertility

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