Estrogen Receptors in Stromal and Epithelial Fractions of the Ventral Prostate of Rats

Purvis, K.; Mørkås, L.; Rui, Hallgeir; Attramadal, Arne

doi:10.3109/01485018508986904

Cited by 9 publications

(3 citation statements)

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“…Scatchard analysis revealed that the hormone appeared to exert effects on one of the two binding components which can be demonstrated in the cytosol, the relatively lower-affinity high-capacity component which appears to be distinct from the classical oestrogen receptor (Purvis et al, 1985). Because the analysis was carried out on pools of tissue it was not possible in this study to obtain statistical proof for the apparent effect on the low affinity component.…”

Section: Discussionmentioning

confidence: 93%

Short-term effects of prolactin on prostatic function in rats with lisuride-induced hypoprolactinaemia

Rui¹,

Haug²,

Mevåg³

et al. 1985

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The effects of a single injection of ovine prolactin on prostatic function were monitored in intact, intact androgenized and castrated-androgenized rats rendered hypoprolactinaemic after 7 days of treatment with a potent dopamine agonist, lisuride. Hypoprolactinaemia was associated with reductions in ventral prostate weight, polyamine levels, lateral lobe zinc and the concentration of the ventral prostate protein prostatein, but an elevation in the level of cytosolic oestradiol binding. Whether these differences attained statistical significance depended on whether the animals were intact, intact-androgenized or castrated-androgenized. With the exception of ventral prostate weight and lateral lobe zinc concentrations, a single injection of prolactin restored or reversed these changes towards control levels within 12 h, which could not be explained by an indirect effect of the hormone on adrenal or testicular function. No effects of lisuride or prolactin were observed with regard to the content of fructose in the coagulating gland or in the degree of prolactin binding to prostatic membranes.

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Section: Discussionmentioning

confidence: 93%

Short-term effects of prolactin on prostatic function in rats with lisuride-induced hypoprolactinaemia

Rui¹,

Haug²,

Mevåg³

et al. 1985

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“…Indeed, a wide variety of hormones and drugs have been shown to induce ODC in other tissues (Russell -1980), and it is unlikely that androgens constitute the only stimulus to its activation. Prostatic epithelium is equipped with other classes of receptors including those for oestrogen (Purvis et al -1985) and prolactin (Charreau et d. -1977) and the latter hormones have been shown to interact with ODC in other organs (Richards -1975;Rorke et al -1984).…”

Section: Discussionmentioning

confidence: 99%

Androgen Interaction with the Polyamine System of the Rat Prostate

et al. 2009

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The ventral and dorsal lobes of the rat prostate contain larger quantities of the aliphatic amines putrescine, spermidine and spennine and higher activities of the enzyme omithine decarboxylase (ODC; EC 4.1.1.17) than other accessory sex glands. In contrast, the coagulating glands and the seminal vesicles contain only small quantities of the amines but the highest activities of the arginase (ARG; EC 3.5.3.1). Lineweaver-Burk plots indicated that the Km-values for ARG in the coagulating gland and ODC in the ventral prostate lobe were 20 mM and 0.2 mM, respectively. Castration decreased ODC and ARG activities to 3 and 50% of control levels, respectively, after 3 days, whilst the Kmvalues were unaffected. Daily administration of 3 mg dihydrotestosterone (DHT) prevented these castrational changes. Oestrogen treatment alone had no effect on the activities of the enzymes, but appeared to exert a synergistic effect with androgen on the ODC. Administration of androgen to intact rats for 7 days caused a dose-related alteration in the ratios of the various amines, particularly the spermine: putrescine ratio. A minor but significant decrease was also recorded in the activity of the ODC, which was mirrored by an increase in the levels of putrescine in the tissue. The data suggest that androgen control of the polyamine pathway is biphasic, first stimulatory and later inhibitory with lesions occurring at the ODC, possibly via short loop feedback of its product putrescine, but also at subsequent enzymic steps in spermidine and spermine biosynthesis. lnteraktion der Androgene mit dem Polyaminstoffwechsel der Rattenprostata Zusammenfassung: Der ventrale und dorsale Lappen der Rattenprostata enthalt grofiere Mengen an aliphatischen Aminen wie Putrescin, Spermidin und Spermin, und auch grofiere Aktivitaten des Enzyms Omithindecarboxylase (ODC; EC 4.1 .I .17) als andere accessorische Geschlechtsdriisen. Im Gegensatz dazu enthalten die Koagulationsdriisen und die Samenblasen nur geringe Mengen an Aminen, aber die hochsten Aktivitaten von Arginase (ARG; EC 3.5.3.1). Berechnungen nach Lineweaver-Burk zeigten, dai3 die Km-Werte fur ARG in der Koagulationsdriise und ODC im ventralen Prostatalappen 20 mM beziehungsweise 50% der Kontrollgruppenwerte nach 3 Tagen, wahrend die Km-Werte nicht beeinflufit waren. Die tagliche Gabe von 3 mg Dihydrotestosteron verhinderte die Veranderungen unter Kastration. Alleinige ostrogenbehandlung hatte keinen Effekt auf die Enzymaktivitaten, abei-es zeigte sich ein synergistischer Effekt zusammen mit Androgen auf die ODC. Wurden gesunde Ratten iiber 7 Tage mit Androgenen behandelt, bewirkte das eine dosisabhangige Veranderung der Aminkonzentrationen, im besonderen das Verhaltnis Sper-Androgen Interaction 135 min zu Putrescin. Ein geringer jedoch signifikanter Abfall wurde auch fur die ODC-Aktivitat registriert, welches sich in einem Anstieg der Putrescin-Werte im Gewebe widerspiegelte. Diese Daten lassen vermuten, d& die durch Androgene bewirkte Kontrolle auf den Polyaminstoffwechsel biphasisch ist, zu Begi...

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“…Estrogen and progestin receptors have also been detected in both epithelium and stroma (173,189). Estrogen receptors appear to be elevated in the stroma (180, 186,187,193,194). Thus, there is now general agreement that stromal cells are clearly important hormone targets which are metabolically active and thus may play a fundamental role in hormonal response.…”

Section: Regulation Of Prostatic Growthmentioning

confidence: 96%

The Endocrinology and Developmental Biology of the Prostate*

et al. 1987

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Estrogen Receptors in Stromal and Epithelial Fractions of the Ventral Prostate of Rats

Abstract: The cytosol from rat ventral prostates contains two estrogen-binding components that can be separated in a glycerol gradient: a highaffinity (KD = 4.2 X lo-'' M), low-capacity

Cited by 9 publications

References 14 publications

Short-term effects of prolactin on prostatic function in rats with lisuride-induced hypoprolactinaemia

Short-term effects of prolactin on prostatic function in rats with lisuride-induced hypoprolactinaemia

Androgen Interaction with the Polyamine System of the Rat Prostate

The Endocrinology and Developmental Biology of the Prostate*

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