Estrogen receptor analyses in breast cancer: Comparison of monoclonal immunohistochemical and biochemical methods

Estrogen and progesterone receptors in breast cancer: Comparison between enzyme immunoassay and computer-assisted image analysis of immunocytochemical assay

“…Several studies have shown good correlation between EIA and DCC (9,12,14,22) and ICA and DCC (2, 11,17,18,20). Recent data have also shown that there is a good correlation between results obtained with EIA and ICA for ER evaluation ( 5 , 6 ) and, more recently, for ER and PR as well (3).…”

Estrogen and progesterone receptors in breast cancer: Comparison between enzyme immunoassay and computer-assisted image analysis of immunocytochemical assay

“…Several studies have shown good correlation between EIA and DCC (9,12,14,22) and ICA and DCC (2, 11,17,18,20). Recent data have also shown that there is a good correlation between results obtained with EIA and ICA for ER evaluation ( 5 , 6 ) and, more recently, for ER and PR as well (3).…”

Assessment of Tissue Estrogen and Progesterone Receptor Levels: A Survey of Current Practice, Techniques, and Quantitation Methods

“…Steroid hormone receptor assay by IHC appears robust enough to maintain the correlation with prognosis established by DCC, despite the many variations discussed. This interpretation is supported by the observation that the majority of studies in the literature using variable techniques, antibodies, titers, and cutoff points still report good correlation of the IHC results with DCC assays and demonstrate good predictive and prognostic value for the test (1,6–17). As in many areas of surgical pathology, reliability may exceed reproducibility (24).…”

“…More recently, ER and PgR assessment by IHC has become popular, if not the predominant technique. Many studies have correlated the results of IHC with those obtained by the ligand binding method (6–12), and have confirmed the relationship of ER and PgR with patient prognosis and response to hormonal therapy (1,14–17). Despite the near uniformity in finding a high correlation between IHC and ligand binding assay results and good predictive value for IHC, these studies have used a variety of antibodies, antibody dilutions, staining and quantitation techniques, and cutoff points (23).…”

“…Many studies have compared the results of immunohistochemically determined steroid receptor values with those obtained by ligand binding analysis. In general, the correlation has been good (6–11). Despite agreement of results derived from individual antibodies used in immunohistochemistry (IHC) determinations and the ligand binding technique, significant variability has been documented in the results obtained by IHC using different commercially available antibodies (12,13).…”

Estrogen and progesterone receptors in epithelial ovarian tumours

“…The significance of this factor is unknown but it is probably the number of unoccupied receptors which determines the effect of hormonal treatment. Many studies have found a high degree of qualitative correlation between the results obtained by the biochemical assay and those of immunocytochemical assays (21). The quantitative correlation between immunocytochemical and biochemical analysis is more controversial (2 l).…”

Estrogen and progesterone receptors in breast cancer: Comparison between enzyme immunoassay and computer-assisted image analysis of immunocytochemical assay

“…Several studies have shown good correlation between EIA and DCC (9,12,14,22) and ICA and DCC (2, 11,17,18,20). Recent data have also shown that there is a good correlation between results obtained with EIA and ICA for ER evaluation ( 5 , 6 ) and, more recently, for ER and PR as well (3).…”

Assessment of Tissue Estrogen and Progesterone Receptor Levels: A Survey of Current Practice, Techniques, and Quantitation Methods

“…Steroid hormone receptor assay by IHC appears robust enough to maintain the correlation with prognosis established by DCC, despite the many variations discussed. This interpretation is supported by the observation that the majority of studies in the literature using variable techniques, antibodies, titers, and cutoff points still report good correlation of the IHC results with DCC assays and demonstrate good predictive and prognostic value for the test (1,6–17). As in many areas of surgical pathology, reliability may exceed reproducibility (24).…”

“…More recently, ER and PgR assessment by IHC has become popular, if not the predominant technique. Many studies have correlated the results of IHC with those obtained by the ligand binding method (6–12), and have confirmed the relationship of ER and PgR with patient prognosis and response to hormonal therapy (1,14–17). Despite the near uniformity in finding a high correlation between IHC and ligand binding assay results and good predictive value for IHC, these studies have used a variety of antibodies, antibody dilutions, staining and quantitation techniques, and cutoff points (23).…”

“…Many studies have compared the results of immunohistochemically determined steroid receptor values with those obtained by ligand binding analysis. In general, the correlation has been good (6–11). Despite agreement of results derived from individual antibodies used in immunohistochemistry (IHC) determinations and the ligand binding technique, significant variability has been documented in the results obtained by IHC using different commercially available antibodies (12,13).…”

Estrogen and progesterone receptors in epithelial ovarian tumours

“…The significance of this factor is unknown but it is probably the number of unoccupied receptors which determines the effect of hormonal treatment. Many studies have found a high degree of qualitative correlation between the results obtained by the biochemical assay and those of immunocytochemical assays (21). The quantitative correlation between immunocytochemical and biochemical analysis is more controversial (2 l).…”

Estrogen and progesterone receptors in breast cancer: Comparison between enzyme immunoassay and computer-assisted image analysis of immunocytochemical assay

“…Several studies have shown good correlation between EIA and DCC (9,12,14,22) and ICA and DCC (2, 11,17,18,20). Recent data have also shown that there is a good correlation between results obtained with EIA and ICA for ER evaluation ( 5 , 6 ) and, more recently, for ER and PR as well (3).…”

Assessment of Tissue Estrogen and Progesterone Receptor Levels: A Survey of Current Practice, Techniques, and Quantitation Methods

“…Steroid hormone receptor assay by IHC appears robust enough to maintain the correlation with prognosis established by DCC, despite the many variations discussed. This interpretation is supported by the observation that the majority of studies in the literature using variable techniques, antibodies, titers, and cutoff points still report good correlation of the IHC results with DCC assays and demonstrate good predictive and prognostic value for the test (1,6–17). As in many areas of surgical pathology, reliability may exceed reproducibility (24).…”

“…More recently, ER and PgR assessment by IHC has become popular, if not the predominant technique. Many studies have correlated the results of IHC with those obtained by the ligand binding method (6–12), and have confirmed the relationship of ER and PgR with patient prognosis and response to hormonal therapy (1,14–17). Despite the near uniformity in finding a high correlation between IHC and ligand binding assay results and good predictive value for IHC, these studies have used a variety of antibodies, antibody dilutions, staining and quantitation techniques, and cutoff points (23).…”

“…Many studies have compared the results of immunohistochemically determined steroid receptor values with those obtained by ligand binding analysis. In general, the correlation has been good (6–11). Despite agreement of results derived from individual antibodies used in immunohistochemistry (IHC) determinations and the ligand binding technique, significant variability has been documented in the results obtained by IHC using different commercially available antibodies (12,13).…”

Estrogen and progesterone receptors in epithelial ovarian tumours

“…The significance of this factor is unknown but it is probably the number of unoccupied receptors which determines the effect of hormonal treatment. Many studies have found a high degree of qualitative correlation between the results obtained by the biochemical assay and those of immunocytochemical assays (21). The quantitative correlation between immunocytochemical and biochemical analysis is more controversial (2 l).…”