Estrogen Mitigates the Negative Effects of Arsenic Contamination in an<i>In Vitro</i>Wound Model

Pinto, Bronson I.; Lujan, Oscar R.; Ramos, Stephan A.; Propper, Catherine R.; Kellar, Robert S.

doi:10.1089/aivt.2017.0020

Cited by 8 publications

(6 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Rippon et al, 2016 demonstrated a counter-intuitive model about healing wounds using the "hyper-hydration" of tissue, in which tissue is hydrated beyond the normally acceptable therapeutic level. Another study has reported that hydrotherapy provides an optimal healing environment through autolytic debridement and absorption of wound fluid, which enables re-epithelialization to occur in an unrestricted fashion (Pinto et al, 2018;Lin et al, 2017). However, in this work, Zamzam water showed potent wound healing properties, which may be due to the presence of a trace level of many minerals.…”

Section: Wound Healing Propertiesmentioning

confidence: 66%

Physical characterization and wound healing properties of Zamzam water

et al. 2022

View full text Add to dashboard Cite

The objective of the study was to evaluate the quality of Zamzam water, holy water for Muslims and consumed for its medicinal value. The present study demonstrates the physicochemical characterization and wound healing property of Zamzam water. The physicochemical characterization of Zamzam water samples was analyzed for dissolved oxygen, pH, conductivity, total dissolved solids, redox potential, zeta potential, polydispersity index, and zeta size. The microbial quality of Zamzam water was also assessed by exposing water samples to open air. In this work, Zamzam water was also screened for the medicinal value through wound healing properties in Wistar rats. Zamzam water exhibited a unique physicochemical characterization with high levels of dissolved oxygen, zeta potential, polydispersity index, redox potential, total dissolved solids, and conductivity before exposure to open air. After open air exposure, Zamzam water resisted the growth of bacteria. The wound healing properties of Zamzam water in vivo showed a 96% of healing effect on 12th day observation. The wound healing was achieved by modulating pro-inflammatory cytokine such as interleukin -1β (IL-1β), interleukin-6 (IL-6), and tumor necrosis factor -α (TNF-α). Followed by the level of apoptosis markers caspase-9 and caspase-3 were reduced. The present study proved that Zamzam water is a good-quality water and showed excellent wound healing property. Therefore, Zamzam water can be used for pharmaceutical formulations.

show abstract

Section: Wound Healing Propertiesmentioning

confidence: 66%

Physical characterization and wound healing properties of Zamzam water

et al. 2022

View full text Add to dashboard Cite

show abstract

“…Application of this assay provides a high-throughput and timely evaluation of cellular migration and may directly translate to complex physiological systems 5,6,7 . For this reason, the proposed bench-top model has been tuned and practiced to evaluate the effects of various therapeutic agents and environmental toxins on wound healing…”

Section: Discussionmentioning

confidence: 99%

“…. Another common technique, described in this study, is the use of inverted light microscopy with phase contrast 6,7 . This alternative to cell dyes and fluorescence allows the user to live track cells without having to terminally fix cells prior to imaging and also allows for capturing images of the same individual wells containing wounded monolayers of cells across time points.…”

mentioning

confidence: 99%

<em>In Vitro</em> Scratch Assay to Demonstrate Effects of Arsenic on Skin Cell Migration

Pinto¹,

Cruz²,

Lujan³

et al. 2019

JoVE

Self Cite

View full text Add to dashboard Cite

Understanding the physiologic mechanisms of wound healing has been the focus of ongoing research for many years. This research directly translates into changes in clinical standards used for treating wounds and decreasing morbidity and mortality for patients. Wound healing is a complex process that requires strategic cell and tissue interaction and function. One of the many critically important functions of wound healing is individual and collective cellular migration. Upon injury, various cells from the blood, surrounding connective, and epithelial tissues rapidly migrate to the wound site by way of chemical and/or physical stimuli. This migration response can largely dictate the outcomes and success of a healing wound. Understanding this specific cellular function is important for translational medicine that can lead to improved wound healing outcomes. Here, we describe a protocol used to better understand cellular migration as it pertains to wound healing, and how changes to the cellular environment can significantly alter this process. In this example study, dermal fibroblasts were grown in media supplemented with fetal bovine serum (FBS) as monolayer cultures in tissue culture flasks. Cells were aseptically transferred into tissue culture treated 12-well plates and grown to 100% confluence. Upon reaching confluence, the cells in the monolayer were vertically scratched using a p200 pipet tip. Arsenic diluted in culture media supplemented with FBS was added to individual wells at environmentally relevant doses ranging 0.1-10 μM. Images were captured every 4 hours (h) over a 24 h period using an inverted light microscope to observe cellular migration (wound closure). Images were individually analyzed using image analysis software, and percent wound closure was calculated. Results demonstrate that arsenic slows down wound healing. This technique provides a rapid and inexpensive first screen for evaluation of the effects of contaminants on wound healing.

show abstract

“…Wound healing assay was performed according to the method of Pinto and Ramenzoni with modification (Pinto BI et al, 2018, Ramenzoni et al, 2017. The CL-6 cells (1.0×10 4 cell/well) were seeded onto a 96-well plate and incubated overnight at 37 o C under 5% CO 2 atmosphere.…”

Section: Wound Healing Assaymentioning

confidence: 99%

Cytotoxicity, Cell Cycle Arrest, and Apoptosis Induction Activity of Ethyl-p-methoxycinnamate in Cholangiocarcinoma Cell

Muhamad

Panrit

Chai่jaroenkul

et al. 2020

Asian Pac J Cancer Prev

View full text Add to dashboard Cite

Objective: To investigate cytotoxic activity of ethyl-p-methoxycinnamate (EPMC) including its effect on p-glycoprotein (multidrug resistance-1: mdr-1 gene) in human cholangiocarcinoma cell. Methods: Cytotoxic activity of EPMC against human cholangiocarcinoma (CL-6), fibroblast (OUMS-36T-1F), and colon cancer (Caco-2) cell lines were assessed using MTT assay. Selectivity index (SI) was determined as the ratio of IC 50 (concentration that inhibits cell growth by 50%) of EPMC in OUMS-36T-1F and that in CL-6 cell. Cell cycle arrest and apoptosis in CL-6 cells were investigated by flow cytometry and fluorescent microscopy. Effect of EPMC on mdr-1 gene expression in CL-6 and Caco-2 was determined by real-time PCR. Results: The median (95% CI) IC 50 values of EPMC in CL-6, OUMS-36T-1F, and Caco-2 were 245.5 (243.1-266.7), 899.60 (855.8-966.3) and 347.0 (340.3-356.9) µg/ml, respectively. The SI value of the compound for the CL-6 cell was 3.70. EPMC at IC 50 inhibited CL-6 cell division and induced apoptosis compared to untreated control. EPMC exposure did not induce mdr-1 gene expression in both CL-6 and Caco-2 cells. Conclusion: The results suggest the potential role of EPMC in cholangiocarcinoma with a low possibility of drug resistance induction.

show abstract

Estrogen Mitigates the Negative Effects of Arsenic Contamination in anIn VitroWound Model

Cited by 8 publications

References 47 publications

Physical characterization and wound healing properties of Zamzam water

Physical characterization and wound healing properties of Zamzam water

<em>In Vitro</em> Scratch Assay to Demonstrate Effects of Arsenic on Skin Cell Migration

Cytotoxicity, Cell Cycle Arrest, and Apoptosis Induction Activity of Ethyl-p-methoxycinnamate in Cholangiocarcinoma Cell

Contact Info

Product

Resources

About