Estrogen-like anabolic effects of cyclic 3′,5′ adenosine monophosphate and other nucleotides in isolated rat uterus

Hechter, Oscar; Yoshinaga, Koji; Halkerston, I. D. K.; Birchall, Kathleen

doi:10.1016/0003-9861(67)90219-6

Cited by 70 publications

(18 citation statements)

References 25 publications

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“…Early reports demonstrated that estrogen administered to ovariectomized rats elevated cAMP in uterine cells [13,23,241 and stimulated uterine adenylate cyclase activity [21], and that theophylline potentiated this estrogen effect uterine tissue adenyl cyclase is high during proestrus and then declines during estrus and metestrus [4]. Thus, it could be postulated that uterine tissue cAMP levels should be elevated prior to detection of rising cAMP levels in the genital tract fluids.…”

Section: Discussionmentioning

confidence: 99%

Vaginal Fluid Adenosine 3',5'-Cyclic Monophosphate (cAMP) in the Rat: Interaction with Sperm cAMP-Dependent Protein Kinase Regulatory Subunits

Atherton

Culver

Seitz

et al. 1986

Archives of Andrology

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Section: Discussionmentioning

confidence: 99%

Vaginal Fluid Adenosine 3',5'-Cyclic Monophosphate (cAMP) in the Rat: Interaction with Sperm cAMP-Dependent Protein Kinase Regulatory Subunits

Atherton

Culver

Seitz

et al. 1986

Archives of Andrology

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“…Adenosine 3',5'-monophosphate (cyclic AMP) has been shown to mimic some of the early anabolic effects of oestradiol in the rat uterus (Hechter, Yoshinaga, Halkerston & Birchall, 1967;Griffin & Szego, 1968;Mohla & Prasad, 1970). The studies reported here were carried out to determine whether cyclic AMP could substitute for the nidatory action of oestradiol in the mouse.…”

Section: Introductionmentioning

confidence: 99%

Implantation in Ovariectomized Mice Treated With Dibutyryl Adenosine 3',5'-Monophosphate (Dibutyryl Cyclic Amp)

Webb¹

1975

Reproduction

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“…It is, therefore, interesting to examine whether the same mediators might be implicated in the growth-promoting action of hormones such as steroids on epithelial tissues. Despite earlier results (8), estrogens do not seem to affect cyclic AMP levels in the uterus (9,10), but treatment with cyclic AMP induces a response that can mimic estrogen effects (11)(12)(13). Indeed, the endometrium contains a cyclic AMPdependent protein kinase (14).…”

mentioning

confidence: 77%

Phosphoprotein Phosphatase Activity Associated with Estrogen-Induced Protein in Rat Uterus

Vokaer

Iacobelli

Kram

1974

Proc. Natl. Acad. Sci. U.S.A.

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Estrogen-induced protein was purified from rat uteri and assayed for several enzymatic activities involved in the metabolism and action of cyclic nucleo tides. No adenylate and guanylate cyclase (EC 4.6 One of the earliest effects of estrogen on rat uterus is the induction within 1 hr after hormone administration, of the synthesis of a specific protein (IP) (1). IP represents a very minor component of uterine soluble proteins that can only be demonstrated on the basis of increased incorporation of labeled amino acids in this protein after estrogen treatment. IP has been purified by ion exchange chromatography and preparative gel electrophoresis as a polypeptide of 45,000 molecular weight (2).The biological function of this induced protein (IP) is still unknown, although it has been implicated by some authors to play a decisive role in the subsequent increase in RNA and protein synthesis involved in the trophic effects of the hormone on its target tissues (3).Cyclic AMP and cyclic GMP control several processes related to cell growth in fibroblastic and lymphoid cell lines in titro (4-7). It is, therefore, interesting to examine whether the same mediators might be implicated in the growth-promoting action of hormones such as steroids on epithelial tissues. Despite earlier results (8), estrogens do not seem to affect cyclic AMP levels in the uterus (9, 10), but treatment with cyclic AMP induces a response that can mimic estrogen effects (11-13). Indeed, the endometrium contains a cyclic AMPdependent protein kinase (14). Although cyclic AMP does not influence the early synthesis of IP (1), cyclic nucleotides might play a role in subsequent effects of estrogens.We have investigated whether IP has enzymatic activity related to the regulation of uterine cell functions by the intracellular mediators, cyclic AMP and cyclic GMP. In this study, we report that IP exhibits a significant phosphoprotein phosphatase (EC 3.1.3.16; phosphoprotein phosphohydrolase) activity strongly inhibited by Zn++ and unaffected by either cyclic AMP or cyclic GMP. This phosphatase activity shows great substrate specificity, being much more efficient on phosphorylated protamine and histone F1 than on phosvitin. MATERIALS AND METHODSIsolation of Purified IP. IP was prepared following the described general procedure (2) with a slight modification. Immature female rats, 21-24 days old (Wistar), were injected intraperitoneally with 5 ug of 17T-estradiol; 1 hr later, the animals were killed and the excised uteri were incubated for 1 hr with [14C]leucine (100 ,Ci/ml). Uteri from control rats were incubated with' [3H]leucine (500 ,Ci/ml). At the termination of the incubation period, control and treated uteri were rinsed and combined with unincubated uteri from 40 to 50 mature virgin female rats. After homogenization in 1.5 mM EDTA (pH 7.6), the homogenates were centrifuged at 105,000 X g for 30 min, and the combined cytosol fractions were applied to a DEAE-cellulose column (1 X 20 cm). After the column was washed, fractionation was performed by...

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Estrogen-like anabolic effects of cyclic 3′,5′ adenosine monophosphate and other nucleotides in isolated rat uterus

Cited by 70 publications

References 25 publications

Vaginal Fluid Adenosine 3',5'-Cyclic Monophosphate (cAMP) in the Rat: Interaction with Sperm cAMP-Dependent Protein Kinase Regulatory Subunits

Vaginal Fluid Adenosine 3',5'-Cyclic Monophosphate (cAMP) in the Rat: Interaction with Sperm cAMP-Dependent Protein Kinase Regulatory Subunits

Implantation in Ovariectomized Mice Treated With Dibutyryl Adenosine 3',5'-Monophosphate (Dibutyryl Cyclic Amp)

Phosphoprotein Phosphatase Activity Associated with Estrogen-Induced Protein in Rat Uterus

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