Estrogen-induced cell signaling in the sexually dimorphic nucleus of the rat preoptic area: Potential involvement of cofilin in actin dynamics for cell migration

Wada‐Kiyama, Yuko; Suzuki, Chiaki; Hamada, Toshihiro; Rai, Dilip; Kiyama, Ryoiti; Kaneda, Makoto; Sakuma, Yasuo

doi:10.1016/j.bbrc.2013.02.117

Cited by 6 publications

(4 citation statements)

References 29 publications

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“…It can also activate Rac/PAK signaling in neurons (14), but the role of this pathway in cofilin phosphorylation and actin polymerization has not yet been directly determined. Similarly, the time dependency for estradiol regulation of these pathways is uncertain.…”

Section: Resultsmentioning

confidence: 99%

Estrogen Regulates Protein Synthesis and Actin Polymerization in Hippocampal Neurons through Different Molecular Mechanisms

Briz

Baudry

2014

Front. Endocrinol.

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Estrogen rapidly modulates hippocampal synaptic plasticity by activating selective membrane-associated receptors. Reorganization of the actin cytoskeleton and stimulation of mammalian target of rapamycin (mTOR)-mediated protein synthesis are two major events required for the consolidation of hippocampal long-term potentiation and memory. Estradiol regulates synaptic plasticity by interacting with both processes, but the underlying molecular mechanisms are not yet fully understood. Here, we used acute rat hippocampal slices to analyze the mechanisms underlying rapid changes in mTOR activity and actin polymerization elicited by estradiol. Estradiol-induced mTOR phosphorylation was preceded by rapid and transient activation of both extracellular signal-regulated kinase (ERK) and protein kinase B (Akt) and by phosphatase and tensin homolog (PTEN) degradation. These effects were prevented by calpain and ERK inhibitors. Estradiol-induced mTOR stimulation did not require activation of classical estrogen receptors (ER), as specific ERα and ERβ agonists (PPT and DPN, respectively) failed to mimic this effect, and ER antagonists could not block it. Estradiol rapidly activated both RhoA and p21-activated kinase (PAK). Furthermore, a specific inhibitor of RhoA kinase (ROCK), H1152, and a potent and specific PAK inhibitor, PF-3758309, blocked estradiol-induced cofilin phosphorylation and actin polymerization. ER antagonists also blocked these effects of estrogen. Consistently, both PPT and DPN stimulated PAK and cofilin phosphorylation as well as actin polymerization. Finally, the effects of estradiol on actin polymerization were insensitive to protein synthesis inhibitors, but its stimulation of mTOR activity was impaired by latrunculin A, a drug that disrupts actin filaments. Taken together, our results indicate that estradiol regulates local protein synthesis and cytoskeletal reorganization via different molecular mechanisms and signaling pathways.

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Section: Resultsmentioning

confidence: 99%

Estrogen Regulates Protein Synthesis and Actin Polymerization in Hippocampal Neurons through Different Molecular Mechanisms

Briz

Baudry

2014

Front. Endocrinol.

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“…This study led to the identification of a new estrogen-responsive signaling pathway, the PKCδ/Rac1/PAK1/LIMK1/cofilin pathway, in the rat preoptic area ( Fig. 2E; [100]), which suggests that estrogen directs cell migration by enhancing actin dynamics to establish sexual dimorphisms. The involvement of cell migration in the differentiation of the brain was investigated and confirmed by live-cell, fluorescent video microscopy [101].…”

Section: Genes Responding To Physiological Estrogensmentioning

confidence: 97%

“…Alternatively, appropriate standardization would be needed to compare data from different DNA microarray platforms or protocols and to solve many of the problems of microarray data, especially when they are used for humans, such as a risk assessment of endocrine disruptors. [77]), zearalenone (panel C; [83]), bisphenol A (panel D; [86]) and estrogen in the rat brain (panel E; [100]). The genes (proteins) related to but not listed as estrogen-responsive genes identified are parenthesized in panel A.…”

Section: Conclusion and Future Prospectsmentioning

confidence: 98%

Estrogen-responsive genes for environmental studies

Kiyama

Zhu

Kawaguchi

et al. 2014

Environmental Technology & Innovation

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“…GPR30 activation phosphorylate the classical ERα, showing that crosstalk with ERα is important in the display of this and other behaviors, many of which are absent in ERα-null mice [ 25 ]. GPR30-mediated phosphorylation may be also involved in the estrogen-dependent masculinization of the POA during development [ 26 ].…”

Section: Neural Axis For Lordosis Behaviormentioning

confidence: 99%

Preoptic and hypothalamic regulation of multi-tiered, chronologically arranged female rat sexual behavior

Sakuma

2023

J Physiol Sci

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As in many mammalian behaviors, sexual behavior exhibits structure. Each modular components of the structure, that are linked together over time, occur in probabilistic manner. Endocrine milieu, in particular sex hormones, define the probability to synchronize the behavior with the production of gametes. Developmental experience and environmental cues affect the hormonal milieu of the brain. This is especially true in female mammals, in which ova mature with certain intervals along with ovarian secretion of sex hormones. Estrogens secreted by mature ovarian follicles support both affiliative and executive components of female sexual behavior. In the absence of the ovarian steroids, females avoid males when possible, or antagonize and reject males when put together. Female sexual behavior is intimately linked with the estrous cycle in many species such that females are only receptive for a brief period at the estrus stage surrounding ovulation. Thus, in the rat, females strongly influence the outcome of mating encounter with a male. Affiliative or solicitatory behavior shown by females in estrus leads to the female adapting the lordosis posture, which is characterized by hindleg postural rigidity and lordotic dorsiflexion of the spine, in response to touch-pressure somatosensory stimuli on the skin of the flanks, rump-tail base, perineum region given by male partner. The posture facilitates intromission and consequently fertilization. Although dependence on estrogens is the most important feature of female rat sexual behavior, cervical probing combined with palpation of the hindquarter skin acts as a supranormal stimulus to elicit lordosis. Thus, lordosis behavior is a hub of multi-tiered, chronologically arranged set of behaviors and estrogen appear to alter excitability of neural network for lordosis.

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Estrogen-induced cell signaling in the sexually dimorphic nucleus of the rat preoptic area: Potential involvement of cofilin in actin dynamics for cell migration

Cited by 6 publications

References 29 publications

Estrogen Regulates Protein Synthesis and Actin Polymerization in Hippocampal Neurons through Different Molecular Mechanisms

Estrogen Regulates Protein Synthesis and Actin Polymerization in Hippocampal Neurons through Different Molecular Mechanisms

Estrogen-responsive genes for environmental studies

Preoptic and hypothalamic regulation of multi-tiered, chronologically arranged female rat sexual behavior

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