Estrogen effects on thyroid iodide uptake and thyroperoxidase activity in normal and ovariectomized rats

Lima, Lívia P.; Barros, I. A.; Lisboa, Patrícia Cristina; Araújo, Rutênio Luiz Castro de; Silva, Alba C.M.; Rosenthal, Doris; Ferreira, Andrea Cláudia Freitas; Carvalho, Denise P.

doi:10.1016/j.steroids.2006.03.007

Cited by 59 publications

(52 citation statements)

References 27 publications

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“…We have previously shown that the measurement of radioiodide uptake 15 min after 125 I-NaI administration (shortterm iodide uptake) reflects iodide transport through the NIS, without influence of in vivo thyroid iodine organification activity (Ferreira et al 2005, Lima et al 2006. Thus, in order to evaluate the in vivo NIS function, the animals received Na-125 I (3700 Bq, i.p., Amersham) 15 min before decapitation.…”

Section: Thyroid Radioiodide Contentmentioning

confidence: 99%

Retinoic acid modulation of thyroid dual oxidase activity in rats and its impact on thyroid iodine organification

Mühlbauer¹,

Silva²,

Marassi³

et al. 2010

Journal of Endocrinology

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The sodium-iodide symporter (NIS) mediates iodide uptake into the thyrocytes, which is important for the diagnosis and therapy of thyroid disorders. Decreased ability to uptake iodide in thyroid carcinomas reduces the efficacy of radioiodine therapy, and retinoic acid (RA) treatment reinduces iodide uptake. The effectiveness of treatment depends not only on iodide uptake but also on the ability of thyrocytes to organify iodine, which is catalyzed by thyroperoxidase (TPO) in the presence of H 2 O 2 . Our goal was to determine the influence of RA on thyroid iodide uptake, iodine organification, and TPO and dual oxidase (DuOx) activities. Normal rats were treated with all-trans-RA or 13-cis-RA (100 or 1500 mg/100 g body weight (b.w.), s.c.) for 14 and 28 days. The 2 h thyroid radioiodine content significantly decreased in rats treated with all-trans-RA (100 mg/100 g b.w.) for 14 days. In this group, NIS function and TPO activity were unchanged, whereas DuOx activity was significantly decreased, which might have contributed to the decrease in iodine organification. Both doses of 13-cis-RA for 28 days increased the 15 min thyroid radioiodine uptake, while the 2 h radioiodide uptake increased only in rats treated with the highest dose of 13-cis-RA. While TPO activity did not change, H 2 O 2 generation was increased in this group, and serum thyroxine levels were normal. Since radioiodine half-life in the thyroid gland is important for treatment efficacy, our results highlight the importance of correctly choosing the RA isomer, the time and the dose of treatment, in order to improve the efficacy of radioiodine therapy.

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Section: Thyroid Radioiodide Contentmentioning

confidence: 99%

Retinoic acid modulation of thyroid dual oxidase activity in rats and its impact on thyroid iodine organification

Mühlbauer¹,

Silva²,

Marassi³

et al. 2010

Journal of Endocrinology

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“…Altogether, these data suggest that estrogen stimulates both NIS and TPO activities in the thyroid of ovariectomized rats. These effects appear not to be mediated by serum TSH, which was unchanged by estrogen, and could probably occur through a direct action of estradiol on these two essential steps of thyroid hormone synthesis (42).…”

Section: Estrogenmentioning

confidence: 88%

“…Radioiodide uptake was also increased in prepubertal animals that received the higher dose of estradiol, whereas TPO activity was unchanged in prepubertal rats treated with estradiol (42). The mechanism underlying the specific action of estradiol on thyroid NIS activity in prepubertal rats remains to be elucidated.…”

Section: Estrogenmentioning

confidence: 95%

The importance of sodium/iodide symporter (NIS) for thyroid cancer management

Carvalho

Ferreira

2007

Arq Bras Endocrinol Metab

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The thyroid gland has the ability to uptake and concentrate iodide, which is a fundamental step in thyroid hormone biosynthesis. Radioiodine has been used as a diagnostic and therapeutic tool for several years. However, the studies related to the mechanisms of iodide transport were only possible after the cloning of the gene that encodes the sodium/iodide symporter (NIS). The studies about the regulation of NIS expression and the possibility of gene therapy with the aim of transferring NIS gene to cells that normally do not express the symporter have also become possible. In the majority of hypofunctioning thyroid nodules, both benign and malignant, NIS gene expression is maintained, but NIS protein is retained in the intracellular compartment. The expression of NIS in non-thyroid tumoral cells in vivo has been possible through the transfer of NIS gene under the control of tissue-specific promoters. Apart from its therapeutic use, NIS has also been used for the localization of metastases by scintigraphy or PET-scan with 124 I. In conclusion, NIS gene cloning led to an important development in the field of thyroid pathophysiology, and has also been fundamental to extend the use of radioiodine for the management of non-thyroid tumors. A glândula tireóide tem capacidade de captar e concentrar iodeto, etapa fundamental na biossíntese dos hormônios tireóideos. O uso de iodo radioativo para fins de diagnóstico e terapia das doenças da tireóide vem sendo feito há muitos anos. Entretanto, somente após a clonagem do gene que codifica o cotransportador de sódio/iodeto (NIS) houve aumento significativo dos estudos relacionados ao mecanismo de transporte de iodeto. Os estudos sobre a regulação da expressão do NIS e a possibilidade de terapia gênica visando à transferência do gene NIS para células que normalmente não expressam esse transportador, foram também viabilizados. Na maior parte dos nódulos tireóideos hipofuncionantes, tanto benignos quanto malignos, a expressão do gene do NIS está presente, mas a proteína NIS fica retida no compartimento intracelular. A transferência do gene usando-se promotores tecido-específicos possibilitou a expressão do NIS em células tumorais não-tireóideas in vivo. Além do seu uso terapêutico, o NIS também vem sendo usado para a localização de metástases tumorais através da cintilografia ou do PET-scan usando-se 124 I. Em conclusão, a clonagem do NIS possibilitou enorme avanço na área de fisiopatologia tireóidea e foi também fundamental para estender o uso do radioiodo para tumores não tireóideos.

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“…In the thyroid gland, E2 provokes a considerable increase in the thyroid weight, stimulates thyroid iodide uptake, enhances thyroperoxidase activity, and increases the level of T3 (Lima et al, 2006). ERK1/2 regulate various cellular activities, such as gene expression, mitosis, differentiation, proliferation, and cell survival/apoptosis (Roberts and Der, 2007;Dunn et al, 2005).…”

Section: Target Molecules Of Estrogen Receptors In the Thyroid Glandmentioning

confidence: 99%

Estrogen Signaling and Thyrocyte Proliferation

Arciuch¹,

Cristofano²

2012

Thyroid and Parathyroid Diseases - New Insights Into Some Old and Some New Issues

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Estrogen effects on thyroid iodide uptake and thyroperoxidase activity in normal and ovariectomized rats

Cited by 59 publications

References 27 publications

Retinoic acid modulation of thyroid dual oxidase activity in rats and its impact on thyroid iodine organification

Retinoic acid modulation of thyroid dual oxidase activity in rats and its impact on thyroid iodine organification

The importance of sodium/iodide symporter (NIS) for thyroid cancer management

Estrogen Signaling and Thyrocyte Proliferation

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