Retinoic acid modulation of thyroid dual oxidase activity in rats and its impact on thyroid iodine organification

Mühlbauer, Mônica; Silva, Alba Cenélia Matos da; Marassi, Michelle P.; Lourenço, Alexandre Lopes; Ferreira, Andrea Cláudia Freitas; Carvalho, Denise P.

doi:10.1677/joe-09-0421

Cited by 19 publications

(15 citation statements)

References 30 publications

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“…Thyroids were homogenized in the same buffer, and then centrifuged at 100,000 g for 1h at 4°C. The pellet was suspended in 0.5 ml of 50 mM sodium phosphate buffer, pH 7.2, containing 0.25 M sucrose, 2 mM MgCl 2 , 5 µg/ml aprotinin and 34.8 µg/ml PMSF and stored at -70°C until H 2 O 2 generation measurements [34]. …”

Section: Methodsmentioning

confidence: 99%

Flavonoid Rutin Increases Thyroid Iodide Uptake in Rats

et al. 2013

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Thyroid iodide uptake through the sodium-iodide symporter (NIS) is not only an essential step for thyroid hormones biosynthesis, but also fundamental for the diagnosis and treatment of different thyroid diseases. However, part of patients with thyroid cancer is refractory to radioiodine therapy, due to reduced ability to uptake iodide, which greatly reduces the chances of survival. Therefore, compounds able to increase thyroid iodide uptake are of great interest. It has been shown that some flavonoids are able to increase iodide uptake and NIS expression in vitro, however, data in vivo are lacking. Flavonoids are polyhydroxyphenolic compounds, found in vegetables present in human diet, and have been shown not only to modulate NIS, but also thyroperoxidase (TPO), the key enzyme in thyroid hormones biosynthesis, besides having antiproliferative effect in thyroid cancer cell lines. Therefore, we aimed to evaluate the effect of some flavonoids on thyroid iodide uptake in Wistar rats in vivo. Among the flavonoids tested, rutin was the only one able to increase thyroid iodide uptake, so we decided to evaluate the effect of this flavonoid on some aspects of thyroid hormones synthesis and metabolism. Rutin led to a slight reduction of serum T4 and T3 without changes in serum thyrotropin (TSH), and significantly increased hypothalamic, pituitary and brown adipose tissue type 2 deiodinase and decreased liver type 1 deiodinase activities. Moreover, rutin treatment increased thyroid iodide uptake probably due to the increment of NIS expression, which might be secondary to increased response to TSH, since TSH receptor expression was increased. Thus, rutin might be useful as an adjuvant in radioiodine therapy, since this flavonoid increased thyroid iodide uptake without greatly affecting thyroid function.

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Section: Methodsmentioning

confidence: 99%

Flavonoid Rutin Increases Thyroid Iodide Uptake in Rats

et al. 2013

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“…Furthermore, such knowledge in mammalian species is still scarce. While VA impact on thyroid homeostasis has been reported in vivo (Silva et al, 2009; Mühlbauer et al, 2010) and in vitro (Fröhlich et al, 2004), only a recent study suggested that TH signaling might also impact VA signaling/homeostasis (Li et al, 2015). …”

Section: Introductionmentioning

confidence: 99%

Vitamin A Affects Flatfish Development in a Thyroid Hormone Signaling and Metamorphic Stage Dependent Manner

et al. 2017

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Vitamin A (VA) and retinoid derivatives are known morphogens controlling vertebrate development. Despite the research effort conducted during the last decade, the precise mechanism of how VA induces post-natal bone changes, and particularly those operating through crosstalk with the thyroid hormones (THs) remain to be fully understood. Since effects and mechanisms seem to be dose and time-dependent, flatfish are an interesting study model as they undergo a characteristic process of metamorphosis driven by THs that can be followed by external appearance. Here, we studied the effects of VA imbalance that might determine Senegalese sole (Solea senegalensis) skeletogenetic phenotype through development of thyroid follicles, THs homeostasis and signaling when a dietary VA excess was specifically provided during pre-, pro- or post-metamorphic stages using enriched rotifers and Artemia as carriers. The increased VA content in enriched live prey was associated to a higher VA content in fish at all developmental stages. Dietary VA content clearly affected thyroid follicle development, T3 and T4 immunoreactive staining, skeletogenesis and mineralization in a dose and time-dependent fashion. Gene expression analysis showed that VA levels modified the mRNA abundance of VA- and TH-specific nuclear receptors at specific developmental stages. Present results provide new and key knowledge to better understand how VA and TH pathways interact at tissue, cellular and nuclear level at different developmental periods in Senegalese sole, unveiling how dietary modulation might determine juvenile phenotype and physiology.

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“…The H 2 O 2 generation was quantified in thyroid particulate fractions by the Amplex red/horseradish peroxidase assay (Molecular Probes, Invitrogen, Carlsbad, CA, USA), which detects the accumulation of a fluorescent oxidized product, as previously described. 43 The excised thyroids were processed according to Mühlbauer et al (2010). 44…”

Section: Thyroid H 2 O 2 Productionmentioning

confidence: 99%

Regulation of thyroid sodium‐iodide symporter in different stages of goiter: Possible involvement of reactive oxygen species

Matos

Penha

Cardoso-Weide

et al. 2017

Clin Exp Pharma Physio

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Na /I symporter (NIS) transports iodide into thyrocytes, a fundamental step for thyroid hormone biosynthesis. Our aim was to evaluate NIS regulation in different status of goitrogenesis and its underlying mechanisms. Wistar rats were treated with methimazole (MMI) for 5 and 21 days, to achieve different status of goiter. We then evaluated the effect of MMI removal for 1 day (R1d), after 5 (R1d-5d) or 21 (R1d-21d) days of MMI treatment. MMI increased thyroid weight, iodide uptake and in vitro TPO activity in a time-dependent way. Although MMI removal evoked a rapid normalization of TPO activity in R1d-5d, it was still high in R1d-21d. On the other hand, iodide uptake was rapidly down-regulated in R1d-21d, but not in R1d-5d, suggesting that the increased TPO activity in R1d-21d led to increased intraglandular organified iodine (I-X), which is known to inhibit iodide uptake. Since TGFβ has been shown to mediate some effects of I-X, we evaluated TGFβ and TGFβ receptor mRNA levels, which were increased in R1d-21d. Moreover, it has been demonstrated that TGFβ stimulates NOX4. Accordingly, our data revealed increased NOX4 expression and H O generation in R1d-21d. Finally, we evaluated the effect of H O on NIS function and mRNA levels in PCCL3 thyroid cell line, which were reduced. Thus, the present study suggests that there is a relationship between the size of the goiter and NIS regulation and that the mechanism might involve I-X, TGFβ, NOX4 and increased ROS production.

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Retinoic acid modulation of thyroid dual oxidase activity in rats and its impact on thyroid iodine organification

Cited by 19 publications

References 30 publications

Flavonoid Rutin Increases Thyroid Iodide Uptake in Rats

Flavonoid Rutin Increases Thyroid Iodide Uptake in Rats

Vitamin A Affects Flatfish Development in a Thyroid Hormone Signaling and Metamorphic Stage Dependent Manner

Regulation of thyroid sodium‐iodide symporter in different stages of goiter: Possible involvement of reactive oxygen species

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