Estrogen and Progesterone Receptor Contents in ThinPrep-Processed Fine-Needle Aspirates of Breast

Leung, Stephen Wan; Bédard, Yvan C.

doi:10.1093/ajcp/112.1.50

Cited by 44 publications

(39 citation statements)

References 5 publications

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“…11,26 In contrast, other reports showed that microwave-stimulated epitope retrieval can be applied successfully on smears fixed in alcohol, formalin, or processed by ThinPrep. 15,16,27,28 The low ER immunoreactivity in our initial smears, however, prompted us to try heat-induced epitope retrieval on all negatively staining smears that had been fixed in 10% formalin, followed by repeat ER staining. A negative-to-positive conversion was observed in 18 of 20 smears with an increased nuclear staining intensity.…”

Section: Discussionmentioning

confidence: 99%

Optimal fixation conditions for immunocytochemical analysis of estrogen receptor in cytologic specimens of breast carcinoma

Gong

Symmans

Krishnamurthy

et al. 2003

Cancer

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Section: Discussionmentioning

confidence: 99%

Optimal fixation conditions for immunocytochemical analysis of estrogen receptor in cytologic specimens of breast carcinoma

Gong

Symmans

Krishnamurthy

et al. 2003

Cancer

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“…The present study shows that the diagnostic accuracy of ThinPrep-processed breast FNA biopsy specimens is as good as conventionally prepared specimens. ThinPrep processing has the added benefits of allowing flow cytometry 42 and immunohistochemistry, 43 -44 including estrogen and progesterone receptors, 45 to be performed. ThinPrep also has been extremely well accepted by clinicians because of the ease of specimen preparation, and it has remained the method of choice at our institution for the processing of breast FNAs…”

Section: Discussionmentioning

confidence: 99%

Breast Fine-Needle Aspiration: A Comparison of ThinPrep and Conventional Smears

Bédard

Pollett

1999

Am J Clin Pathol

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A b s t r a c t Fine-needle aspiration (FNA) of the breast has been used in our institution since 1969. In August 1993 Multiple studies and reviews have shown that fine-needle aspiration (FNA) of the breast is a safe, useful tool for the examination of breast lesions.1-5 Unfortunately, the unsatisfactory rates range from 0% to 32%, often necessitating repeated aspirations, prolonged patient anxiety, and delay in diagnosis. Breast FNA has been used at our institution since 1969, where it is performed as an outpatient clinic procedure. The aspirates were prepared using a conventional smear method until August 1993 when ThinPrep (Cytyc Corp, Boxborough, MA) processing of breast FNA material was introduced in an attempt to reduce the air-drying artifact often seen in the conventionally smeared specimens. The present study compares the cytohistologic correlation and unsatisfactory rates of breast FNA biopsy specimens that have been conventionally prepared with those processed using the ThinPrep method. MethodsThe results from all breast FNA biopsies from 1969 to 1976 and January 1, 1980, to September 30, 1997, were used. Cytohistologic correlations previously compiled for publication 6 and internal review were available.

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“…Excellent results can also be achieved using the LBC method. Using TP slides from malignant breast aspirates, Leung and Bėdard [31] reported an overall accuracy of 97 and 89% for the detection of ER and PR, respectively. In another study, stronger immunostaining was observed on TP slides in comparison to histological samples [17].…”

Section: Ancillary Techniquesmentioning

confidence: 99%

“…Although not statistically significant, a higher number of cases positive for ER and PR was detected on slides prepared by the TP technique in comparison to CS [32]. Microwave antigen retrieval pretreatment before immunostaining is usually advocated for TP slides [31,32]. In fact, a recent multinational study demonstrated a great variability in the detection of ER and PR in breast FNA specimens because of the different types of cytological preparations and the diverse methods of fixation and immunostaining protocols used by the laboratory participants.…”

Section: Ancillary Techniquesmentioning

confidence: 99%

Liquid-Based Cytology in Fine-Needle Aspiration of Breast Lesions: A Review

Gerhard

Schmitt²

2014

Acta Cytologica

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Objective: Fine-needle aspiration (FNA) is a safe and cost-effective technique for the diagnosis of breast lesions, especially when correlated with clinical and imaging studies. However, the success of breast FNA is highly dependent on the adequate preparation of cytological conventional smears (CS). The liquid-based cytology (LBC) technique consists of an automated method for preparing thin-layer cytological samples from cell suspensions collected in alcohol-based preservative. LBC is designed to improve CS by avoiding limiting factors such as obscuring material, air-drying and smearing artifacts. Study Design: We performed a review of the published literature about LBC applied to breast FNA. Results: LBC preparations of breast aspirates demonstrated better cellular preservation, less cell overlapping and elimination of blood and excessive inflammation compared to CS. Conversely, alterations in architecture and cell morphology as well as loss of myoepithelial cells and stromal elements have been described in LBC specimens, requiring training before applying this technique for diagnosis. Studies have shown a similar accuracy between LBC and CS for the diagnosis of breast lesions. LBC also permits the use of residual material for ancillary tests, which is an important advantage compared to CS. Conclusions: LBC can be safely applied to breast FNA, showing a similar diagnostic accuracy to CS.

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Estrogen and Progesterone Receptor Contents in ThinPrep-Processed Fine-Needle Aspirates of Breast

Abstract: A b s t r a c t

Cited by 44 publications

References 5 publications

Optimal fixation conditions for immunocytochemical analysis of estrogen receptor in cytologic specimens of breast carcinoma

Optimal fixation conditions for immunocytochemical analysis of estrogen receptor in cytologic specimens of breast carcinoma

Breast Fine-Needle Aspiration: A Comparison of ThinPrep and Conventional Smears

Liquid-Based Cytology in Fine-Needle Aspiration of Breast Lesions: A Review

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