Estrogen and Luteinizing Hormone Secretion in Diverse Primate Species from Simplified Urinary Analysis

Abstract

A practical approach for monitoring urinary estrogen and luteinizing hormone (LH) in various primate species is described. Total immunoreactive estrogen (E() levels, measured without chromatography, provided a convenient estimation of estrone, estradiol and estriol as the sum of their separated parts. Bioactive LH in urine was measured by the rat interstitial cell testosterone (RICT) in vitro bioassay. Correction for urinary creatinine content allowed for the comparison of hormone production from small volumes of urine collected from single daily voidings. Using these methods, urinary Et and LH profiles through the reproductive cycles of a human, gorilla, chimpanzee and capuchin monkey have been determined.

Comparison of urinary oestrogen excretion in South American primates

“…This paper extends the use of a recent methodological approach (Hodges et al, 1979) (Czekala et ai, 1980). Isolated fractions containing the 'unidentified' oestrogen from capuchin urine are active in the rat uterine cytosol receptor assay (unpublished data).…”

“…Data on the capuchin cycle have been published (Hodges et al, 1979) but hormone profiles in the spider monkey and white-lipped tamarin have not been previously reported. The cycle lengths for the 2 females studied are similar to values from other studies of New World monkeys (see Preslock et al, 1973;Hearn et al, 1978;Bonney et al, 1979).…”

“…After hydrolysis of 0-5 ml urine, the hydrolysates were separated into four 0-1 ml aliquots. One aliquot was used for measurement of total immunoreactive oestrogen as previously described (Hodges, Czekala & Lasley, 1979 51-0 ± 2-2, 63-0 ± 6-1 and 59 ± 5-3% respectively (4 experiments). (Tulchinsky & Abraham, 1971).…”

Urinary Excretion of Oestradiol-17β in the Female Cycle of Goeldi’s Monkeys (Callimico goeldii)

“…Such a trough has not been reported previously for oestrogen cycles de termined for other New World monkeys: Saimiri [35,36], Aotus [37][38][39][40], Cebus [41][42][43], Saguinus [19,20], Callithrix [6,7,15]. Nevertheless, if urine samples are not col lected regularly every day, such a transitory trough might easily be missed.…”

Avaliação da eficiência da eletroforese capilar como técnica analítica na prospecção de metabólitos de esteróides em extratos fecais de onça-pintada (Panthera onca)

“…Salientando ainda a sobreposição na biossíntese de esteróides pela adrenal, acrescente-se que também a estrona, a progesterona e a 17α-diidroprogesterona foram localizadas entre os inúmeros esteróides da adrenal (FRIEDEN;LIPNER, 1975 Por mais de 25 anos, laboratórios independentes desenvolveram técnicas pioneiras para avaliação de padrões hormonais em fezes e urina ( HODGES et al, 1979;BAMBERG et al, 1984;MONFORT et al, 1990;KIRKPATRICK 1991;GARNIERA et al, 1998;SCHWARZENBERGER et al, 1998) ou mesmo em saliva CALLISON 1996). Metabólitos hormonais excretados nas fezes e urina refletem acuradamente os padrões hormonais no sangue, com, é claro, a demora apropriada na passagem do sangue para a excreta.…”