Estradiol regulates estrogen receptor mRNA stability

Saceda, Miguel; Lindsey, Ralph K.; Solomon, Harrison B.; Angeloni, Stephen V.; Martin, Mary Beth

doi:10.1016/s0960-0760(98)00049-1

Cited by 68 publications

(42 citation statements)

References 45 publications

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“…Estrogen also increased ERa mRNA levels in ZR-75 (Clayton et al, 1997) and Ishikawa cells (Robertson et al, 2002). However, estrogen treatment decreased ERa mRNA in ECC-1 (Farnell and Ing, 2003) and MCF-7 cells (Saceda et al, 1998). This effect was believed to be caused by the relatively low stability of ERa mRNA.…”

Section: Discussionmentioning

confidence: 94%

“…Estrogen may stabilize endometrial ER mRNAs by inducing proteins to bind to specific sequences in the 3'-UTR. However, studies show that treatment with estrogen induces down-regulation of ERα mRNA that appears to be caused by its reduced stability (Borras et al, 1994;Saceda et al, 1998). Relatively little is known about the ability of estrogen to regulate the expression of ERs in pigeon oviduct epithelial cells (POECs).…”

Section: Introductionmentioning

confidence: 99%

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Molecular cloning, expression, and regulation of estrogen receptors in pigeon oviduct epithelial cells

Zhang¹,

Chen²,

Li³

et al. 2014

Genet. Mol. Res.

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ABSTRACT. Estrogen regulates reproductive behavior and drives the proliferation and differentiation of several cell types. These physiological functions of estrogen are mediated by estrogen receptors (ERs), and each ER isoform plays a distinct role. To clarify the molecular mechanism of estrogen action and to evaluate the effect of ERs on the secretion of ovalbumin (OVA) in pigeon oviduct epithelial cells (POECs), we determined the complete coding sequences encoding ER alpha (ERα) and ER beta (ERβ) in pigeons. The abundance of pigeon ERα and ERβ mRNA was detected using quantitative polymerase chain reaction. These results revealed that pigeon ERα is highly expressed in the oviduct, while pigeon ERb is highly expressed in the ovary and kidney. We hypothesize that ERα mRNA predominates over that of ERβ in the oviduct. The expression of ERα can be down- 1927©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 13 (1): 1926-1937 (2014 Characterization and regulation of pigeon ERs regulated by 17β-estradiol, and the knockdown of ERα promoted OVA mRNA expression in cultured POECs, indicating that ERα may play an important role in OVA secretion.

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Section: Discussionmentioning

confidence: 94%

Section: Introductionmentioning

confidence: 99%

Molecular cloning, expression, and regulation of estrogen receptors in pigeon oviduct epithelial cells

Zhang¹,

Chen²,

Li³

et al. 2014

Genet. Mol. Res.

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“…In view of the reduced E max , this may seem to be an apparent contradiction. Estrogen does, however, in some tissues decrease the half-life of mRNA molecules (El Meskini et al 1997, Saceda et al 1998 and therefore the net result of estrogen domination may be an attenuated translation of 2 AR transcripts. In addition, mRNA reduction as a mechanism of receptor down-regulation has primarily been observed in cell culture lines, where rapid cell division necessitates continuous receptor synthesis (Hadcock & Malbon 1988, Bouvier et al 1989.…”

Section: Discussionmentioning

confidence: 99%

Desensitization of beta2-adrenoceptor function in non-pregnant rat myometrium is modulated by sex steroids

Engstrøm

Vilhardt²,

Bratholm³

et al. 2001

Journal of Endocrinology

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The effects of in vivo treatment with estrogen and progesterone on isoproterenol-induced uterine relaxation and 2 -adrenoceptor ( 2 AR) mRNA production in nonpregnant rat myometrium were investigated. Whether homologous myometrial desensitization of 2 AR function was dependent on or modulated by the two steroids was also examined.Estrogen treatment alone or in combination with progesterone reduced maximal relaxation (E max ) of isolated uterine strips subsequently challenged with isoproterenol whereas progesterone alone had no effect on this parameter. The reduction was accompanied by an enhanced 2 AR mRNA concentration. The concentration of isoproterenol giving half-maximal relaxing response (EC 50 ) increased following estrogen treatment and this effect was curbed by progesterone.Isoproterenol had no effect on 2 AR transcription irrespective of the steroid regimes employed. E max of isolated uterine strips was reduced following prolonged in vivo treatment with isoproterenol but the effect was found only when estrogen alone was administered concomitantly. Finally, in vivo treatment with isoproterenol increased EC 50 of uterine strips subsequently stimulated with isoproterenol in vitro. This effect was independent of steroid treatment.We conclude that homologous desensitization of 2 AR function in non-pregnant rat myometrium in terms of sensitivity (EC 50 ) is independent of sex steroids but in terms of maximal response (E max ) occurs only in the presence of estrogen. We speculate whether progesterone withdrawal in connection with the well-known estrogen dominance at rat parturition may strengthen the desensitization induced by 2 AR activation and thus contribute to the transformation of the uterus from a quiescent to a highly contractile organ.

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“…The most common model for studying autoregulation of ER gene expression is the MCF7 breast cancer cell line. However, it shows down-regulation of ER mRNA and protein with E 2 treatment and the effect appears to be due to reduced stability of ER mRNA (Saceda et al 1988, Berkenstam et al 1989, Ree et al 1989, Borras et al 1994, Saceda et al 1998. This contrasts with the natural up-regulation of ER gene expression by estrogens in uterus and breast during estrous and menstrual cycles (Anderson et al 1998).…”

Section: Introductionmentioning

confidence: 99%

Estradiol up-regulates estrogen receptor messenger ribonucleic acid in endometrial carcinoma (Ishikawa) cells by stabilizing the message

Robertson

Farnell²,

Lindahl³

et al. 2002

Journal of Molecular Endocrinology

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Estrogens up-regulate expression of the estrogen receptor alpha (ER) gene in most mammalian tissues studied. Using the ovariectomized ewe as a model, we determined that estradiol (E 2 ) acted post-transcriptionally to increase endometrial ER mRNA concentrations by enhancing the stability of the message. The purpose of this study was to determine whether a similar E 2 effect occurs in Ishikawa cells, a well-differentiated human endometrial adenocarcinoma cell line. The presence and function of ER protein in Ishikawa cells was demonstrated by transactivation of a transfected plasmid (ERE 2 tkCAT) in response to 10 −9 M E 2 , resulting in a 550% increase in reporter gene RNA. Ishikawa cells also responded to E 2 by up-regulating their ER mRNA concentration an average of 100% between 7 and 24 h of treatment. The effect of E 2 on ER mRNA stability was measured after blocking transcription with actinomycin D to find that the half-life increased from 6 to 10 h in control and E 2 -treated cells respectively. These results are consistent with cell-free studies which showed significant enhancement of the half-life of radiolabeled ER 3′ untranslated region (3′UTR) RNA in extracts from E 2 -treated cells versus those from control cells. Thus, Ishikawa cells provide a relevant model system for the study of E 2 -regulated endometrial gene expression.

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Estradiol regulates estrogen receptor mRNA stability

Cited by 68 publications

References 45 publications

Molecular cloning, expression, and regulation of estrogen receptors in pigeon oviduct epithelial cells

Molecular cloning, expression, and regulation of estrogen receptors in pigeon oviduct epithelial cells

Desensitization of beta2-adrenoceptor function in non-pregnant rat myometrium is modulated by sex steroids

Estradiol up-regulates estrogen receptor messenger ribonucleic acid in endometrial carcinoma (Ishikawa) cells by stabilizing the message

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