Estradiol-Cellular Interaction in Tissue Culture.

Abstract

N-methylhydrazine. Their inhibitory action is approximaiely 40 times higher than, e.g., that of L-a-methyldopa.

Abrogated Thymoma Development and Increased Amyloid Development in Estrogenized Mice Grafted Spleen and Bone Marrow Cells

“…A great part of the grafted cells are probably quickly being removed by host macrophages. Perhaps estrogen may increase the breakdown of donor cells ( 3 ) and, in addition, impede the division of recipient macrophages (15,16), something which may be relevant to amyloid development (22,23).…”

“…Blood lymphocytes are, however, only moderately reduced in number and their response to PAH does not point to an inferior function of the lymphocytes in cisrculation. A more important alteration may be a reduced supply of marrow-derived cells to the thymus ( 7 , 14) due to the mitosis-inhibiting effect of estrogen (15,16) and/or the reduced volume of the marrow cavity in estrogen-treated mice (17). The latter possibility is rendered less likely by the observation that there is no correlation between the tendency to skeletal changes and thymoma development in different mouse strains after estrogen treatment ( 18).…”

EFFECTS OF 17-ß-ESTRADIOL AND OF GROWTH HORMONE ON THE PRODUCTION OF ACID MUCOPOLYSACCHARIDES BY HUMAN EMBRYONAL FIBROBLASTS IN VITRO

“…This is in agreement with the observation that in cultures of with 17-B-estradiol with the GI of the control cultures, it appears that the hormone has caused a depression of the growth rate. Several reports in the past have indicated that estradiol exerts in vitro an inhibitory effect on the growth of various cell types (12)(13)(14)(15)(16). With the exception of an inhibitory effect exerted by estradiol on the incorporation of S '5 in cartilage and aorta of rats (17,18), by and large the estrogens seem to induce an increase in AMPS of the ground substance of various organs (10,(19)(20)(21)(22)(23)(24)(25).…”

Tissue Culture in the Study of the Effects of Drugs

“…Kuchler and his co-workers have put forward an interesting theory on the mechanism whereby the corticosteroids achieve their effects (158,159). The writers postulate, on the evidence obtained from experiments with synthetic steroids, such as estradiol and stilbestrol, that the drug molecules form a monolayer on the surface of the cell, thus preventing by occlusion of active sites, the transfer of ionized or highly polar substances across the cell membrane, as for example, amino acids.…”