Estimativa de validade de um novo método de isolamento de vírus rábico

Nogueira, Yeda L.

doi:10.1590/s0034-89102004000200023

Cited by 5 publications

(3 citation statements)

References 14 publications

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“…12,14,15 In the case of the IFA-BM, used in visceral leishmaniasis surveillance and control programs, the 1:40 dilution is usually recommended as dilution cut-off, although considering the 1+ reading as positive. Thus, the estimated sensitivity and specificity value of 57.50% results in a difference in specificity of almost 40% and, after reclassification, specificity was 97.50%.…”

Section: Discussionmentioning

confidence: 99%

Análise TG-ROC de testes de imunofluorescência no diagnóstico de leishmaniose visceral canina

et al. 2009

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TG-ROC analysis of immunofluorescence assays in canine visceral leishmaniasis diagnosis ABSTRACT OBJECTIVE:To analyze the accuracy of the diagnosis of two protocols of indirect immunofluorescence assays for canine visceral leishmaniasis. METHODS:Dogs from the seroepidemiological survey conducted in an endemic area of the cities of Araçatuba and Andradina, in Northwestern São Paulo state, in 2003, and in a non-endemic area of the metropolitan region of São Paulo, were used to assess two protocols of indirect immunofluorescence assay (IFA) for leishmaniasis: one using a Leishmania major heterologous antigen (IFA-BM) and another using a Leishmania chagasi homologous antigen (IFA-CH). Two-graph receiver operating characteristic (TG-ROC) analysis was used to estimate accuracy. TG-ROC analysis compared 1:20 dilution readings of the homologous antigen (IFA-CH), considered as reference test, with IFA-BM dilutions (heterologous antigen). RESULTS:The 1:20 dilution used in the IFA-CH test showed the best contingency coefficient (0.755) and the highest strength of association between the two variables studied (chi-square=124.3). Thus, it was considered the test reference dilution in comparisons with different IFA-BM test dilutions. The best IFA-BM results were obtained from 1:40 dilutions with the best contingency coefficient (0.680) and highest strength of association (chi-square=80.8). With the change in the cut-off point, recommended for the IFA-BM 1:40 dilution in this analysis, the specificity parameter value rose from 57.5% to 97.7%, even though the 1:80 dilution showed the best sensitivity estimate (80.2%), with the new cut-off point.CONCLUSIONS: TG-ROC analysis can provide important information about diagnostic tests, in addition to offering suggestions on cut-off points that can improve test sensitivity and specificity estimates and assessing these tests in terms of the best cost-benefit ratio.

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Section: Discussionmentioning

confidence: 99%

Análise TG-ROC de testes de imunofluorescência no diagnóstico de leishmaniose visceral canina

et al. 2009

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“…Of these samples, three (50%) were also positive on the RT‐PCR test, suggesting that they were not false positives. The other three samples were positive solely on RTCIT, which could indicate false‐positive results possibly related to contamination or differences in reading at the microscope (Nogueira, 2004) WHO et al., 2018). However, it is important to point out that in this study, MIT was performed later than RTCIT, and viral viability could have been lost due to more extended storage and the fact that the samples were thawed for RTCIT, RT‐PCR and FTA ® imprint.…”

Section: Discussionmentioning

confidence: 99%

“…Animal use in science should be carefully evaluated to be reduced as much as possible, especially when alternative methods are available (WHO et al., 2018). Nonetheless, implementing new techniques should be carried out with caution so that parameters such as applicability and reliability are accurate while also understanding that no test will be flawless (Nogueira, 2004). In the present study, we validated reliable diagnostic tools, which will allow the reduction of animal use in rabies diagnosis.…”

Section: Discussionmentioning

confidence: 99%

Laboratory validation of confirmatory tests for rabies diagnosis: Approaches to reduce animal use and facilitate sample collection

Cappelari

Godinho

Silva

et al. 2022

Transbounding Emerging Dis

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Rabies is an encephalitis caused by rabies virus, whose transmission occurs upon contact with infected animals' saliva. The diagnosis is usually performed post-mortem through a direct fluorescent antibody test (DFAT). If the DFAT results are negative, they must be confirmed with an isolation test, usually the mouse inoculation test (MIT), which implies the suffering and death of the animals, high costs and most importantly, up to 28 days to confirm a negative result. Another issue related to rabies diagnosis is the sample collection and storage, which is critical for the rabies virus' RNA genome.Thus, this study aimed to evaluate (i) reverse transcriptase polymerase chain reaction (RT-PCR) and Rabies Tissue Culture Infection Tests (RTCIT) in comparison to DFAT and MIT and (ii) FTA ® cards as an alternative sample collection and preservation method.Eighty animal samples were evaluated through DFAT, RTCIT and RT-PCR; MIT was performed only in DFAT-negative samples. FTA ® cards were evaluated with a subset of 64

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Nyctinomops laticaudatus bat-associated Rabies virus causes disease with a shorter clinical period and has lower pathogenic potential than strains isolated from wild canids

Fuoco¹,

Fernandes²,

Guedes³

et al. 2019

Arch Virol

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Estimativa de validade de um novo método de isolamento de vírus rábico

Abstract: Objective

Cited by 5 publications

References 14 publications

Análise TG-ROC de testes de imunofluorescência no diagnóstico de leishmaniose visceral canina

Análise TG-ROC de testes de imunofluorescência no diagnóstico de leishmaniose visceral canina

Laboratory validation of confirmatory tests for rabies diagnosis: Approaches to reduce animal use and facilitate sample collection

Nyctinomops laticaudatus bat-associated Rabies virus causes disease with a shorter clinical period and has lower pathogenic potential than strains isolated from wild canids

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