Estimativa da concentração espermática do sêmen de peixe pelo método de espermatócrito

Sanches, Eduardo Antônio; Marcos, Ronan Maciel; Baggio, Diego Mendes; Tessaro, Lucélia; Balen, Rafael Ernesto; Bombardelli, Robie Allan

doi:10.1590/s1516-35982011000600001

Cited by 22 publications

(9 citation statements)

References 11 publications

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“…In mammals, higher STR values could reflect in a better straightness capacity of the sperm cells, which could favour the transit of the sperm through the uterus and oviducts and hence, fertilization capacity (Arruda, Ball, Gravance, & Liu, ). However, in fish, the function of this variable is poorly known and its effects must be studied (Sanches et al., ). According to Rurangwa et al.…”

Section: Discussionmentioning

confidence: 99%

Effects of supplemental dietary vitamin C on quality of semen from Nile tilapia (Oreochromis niloticus) breeders

Sarmento

Martins

Costa

et al. 2016

Reprod Domestic Animals

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The objective of this study was to evaluate the effects of vitamin C on growth and quality of semen from Oreochromis niloticus breeders. One hundred and sixty males were fed with different levels of vitamin C (0, 261, 599 and 942 mg/kg diet). The higher weight values were recorded for 599 (166 g) and 942 (175 g) mg of vitamin C/kg diet. Sperm motility, vigour and concentration were higher with 599 and 942 mg of vitamin C/kg diet. The semen volume, gonadosomatic index and plasma protein data from the last week showed a direct relationship with increasing levels of vitamin C. No changes were observed in the hepatosomatic index and blood glucose. The haematocrit and erythrocyte showed higher values estimated by equations derived at 850 and 638 mg vitamin C/kg diet, respectively. The leucocytes were inversely proportional to the increasing levels of vitamin C. After 100 days of feeding, animals fed the diet containing 942 mg vitamin C/kg diet had higher sperm motility, linearity, curvilinear velocity, straight line velocity and average path velocity (p < .05). Higher values of beat cross-frequency were observed in broodfish fed diets containing 942 and 599 mg vitamin C/kg. The different vitamin C levels did not cause differences in straightness, lateral head displacement and sperm morphology. For Nile tilapia males on intensive rearing and handling conditions, vitamin C levels between 599 and 942 mg/kg may be used for a better performance and quality of semen.

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Section: Discussionmentioning

confidence: 99%

Effects of supplemental dietary vitamin C on quality of semen from Nile tilapia (Oreochromis niloticus) breeders

Sarmento

Martins

Costa

et al. 2016

Reprod Domestic Animals

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“…The hormonal doses vary greatly, mainly in males, taking into account factors such as species, number application of latency time and the hormone used. Thus, we can find, in the literature, concentrations used from 1 to 6 mg/kg of pituitary brute extract of Cyprinus carpio (Maria et al, 2006;Oliveira et al, 2007;Sanches et al, 2011;Viveiros et al, 2011Viveiros et al, , 2012, 3-5 UI/g of fish of human chorionic gonadotropin (HCG) (Fogli da Silveira et al, 1990;Kavamoto et al, 1996;Andrade-Talmelli et al, 2001;Caneppele et al, 2009) and in the case of GnRHm analogues, approximately 0.1-1.0 pellet/fish kg (Kucharczyk et al, 2001;Pardo-Carrasco et al, 2006;Carneiro & Mikos, 2008;Cejko et al, 2010;Targońska et al, 2011). D-Ala 6 , Pro 9 Net-mGnRH has already been tested by many researchers (i.e.…”

Section: Discussionmentioning

confidence: 99%

Hormonal induction of Brycon cephalus (Characiformes, Characidae) to spermiation using D-ala6, pro9net-mGnRH + metoclopramide

Bashiyo‐Silva

Costa

Ribeiro

et al. 2015

Zygote

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This study aimed to establish a hormonal induction protocol for spermiation of Brycon cephalus males, using Ala6, Pro9Net-mGnRH + metoclopramide (Ovopel®). Thus, 20 males were used divided into three inductor treatments [⅓ pellet/kg (T1), ⅔ pellet/kg (T2) and 1⅓ pellet/kg (T3)] and one control group (CO), which only received physiological solution applications (0.9% NaCl). All treatments were applied in a single dose. For evaluation of the availability of the treatment, the following seminal parameters were analyzed: seminal volume, subjective spermatic motility, duration of motility, pH, osmolality and spermatic concentration. T3 showed the highest seminal volume (4.66 ± 1.52 ml), and was significantly different in comparison with T1 (2.0 ± 0.9 ml), T2 (3.5 ± 1.3 ml) and CO (2.3 ± 1.2 ml). In relation to spermatic motility, T2 and T3 showed significantly higher levels [5, (81-100%)]. However, T3 showed significantly lower average sperm motility duration than T1, T2 and CO (30 ± 7 s; 28 ± 6 s; 32 ± 8 s, respectively). With regard to the seminal parameters of spermatic concentration, pH and osmolality, no significant variation was verified among treatments. In conclusion, mGnRH + metoclopramide used for hormonal induction of B. cephalus reproduction does not induce changes related to spermatic concentration, pH and osmolality parameters of the seminal fluid and the most adequate doses among tested treatments were ⅔ pellet/kg live fish.

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“…Briefly, sperm concentration was determined through the observation of a 10 µl aliquot of the diluted sperm in a Neubauer‐type hemocytometer chamber (Boeco, Hamburg, Germany) under a light microscope (Olympus ® CX22LED, Tokyo, Japan) at ×400 magnification. The number of sperm cells visualized on ten squares from the chamber and the dilution was considered to calculate sperm concentration (Sanches et al, 2011). Fresh sperm morphologic analysis was performed as described below.…”

Section: Methodsmentioning

confidence: 99%

Sperm cryopreservation of Prochilodus lineatus throughout the same reproductive season

et al. 2021

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The aim of this study was to determine the effect of seasonality on post‐thaw sperm quality in Prochilodus lineatus. Sperm from 43 males was cryopreserved throughout the reproductive season (November–March). Fresh sperm quality, concentration, volume, seminal plasma pH, osmolality and ion concentration were analysed. Post‐thaw sperm was analysed for its motility rate, velocities, beat‐cross frequency, cell morphology, oxidative stress and fertilization capacity. Prochilodus lineatus fresh sperm yielded high‐quality during the reproductive period (motility >80%). Post‐thaw sperm quality changed throughout the season, with higher motility (63.2%–72.3%) and curvilinear velocity (VCL) (55.9–59.2 µm/s) from December to March. Sperm concentration was negatively correlated with post‐thaw sperm motility and VCL, and positively correlated with seminal plasma Ca2+ concentration, catalase activity (CAT) and reactive oxygen species (ROS). Seminal plasma Ca2+ was positively correlated with ROS and negatively correlated with post‐thaw sperm motility. Post‐thaw sperm CAT and fertility correlated positively. Although post‐thaw sperm quality was reduced in November, the increased CAT was efficient in maintaining its fertilization capacity. In order to face seasonal influence, the optimal period to cryopreserve P. lineatus spermatozoa is from December to March, when sperm exhibits characteristics which make spermatozoa more prone to resist cryodamage.

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Estimativa da concentração espermática do sêmen de peixe pelo método de espermatócrito

Cited by 22 publications

References 11 publications

Effects of supplemental dietary vitamin C on quality of semen from Nile tilapia (Oreochromis niloticus) breeders

Effects of supplemental dietary vitamin C on quality of semen from Nile tilapia (Oreochromis niloticus) breeders

Hormonal induction of Brycon cephalus (Characiformes, Characidae) to spermiation using D-ala6, pro9net-mGnRH + metoclopramide

Sperm cryopreservation of Prochilodus lineatus throughout the same reproductive season

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