Estimation of total alkaloid in Chitrakadivati by UV-Spectrophotometer

Abstract

Background:Herbal formulation standardization by adopting newer technique is need of the hour in the field of Ayurvedic pharmaceutical industry. As very few reports exist. These kind of studies would certainly widen the herbal research area. Chitrakadivati is one such popular herbal formulation used in Ayurveda. Many of its ingredients are known for presence of alkaloids.Methodology:Presence of alkaloid was tested qualitatively by Dragondroff's method then subjected to quantitative estimation by UV-Spectrophotometer. This method is based on the reaction between alkaloid and bromocresol green (BCG).Results and Conclusion:Study discloses that out of 16 ingredients, 9 contain alkaloid. Chitrakadivati has shown 0.16% of concentration of alkaloid and which is significantly higher than it's individual ingredients.

Concentraciones de alcaloides, glucósidos cianogénicos, polifenoles y saponinas en plantas medicinales seleccionadas en Ecuador y su relación con la toxicidad aguda contra Artemia salina

“…Determinación cuantitativa de alcaloides: Se utilizó el método basado en la reacción con verde de bromocresol (BCG) (Shamsa, Monsef, Ghamooshi, & Verdian-rizi, 2008;Ajanal, Gundkalle, & Nayak, 2012), a partir de una solución de atropina (1 mg/mL), diluida 1:10, se prepararon seis soluciones (0.01-0.1 mg/mL) para la elaboración de la curva de calibración. Para las determinaciones en los extractos de las hojas de las plantas, 5 mL de cada extracto se transfirieron a un embudo de separación, al cual se le agregaron 5 mL de solución de BCG y 5 mL una solución buffer de fosfato a pH 4.7.…”

Phytochemical Evaluation and In vitro Antioxidant Activity of Various Solvent Extracts of Leucas aspera (Willd.) Link Leaves

“…12 Small quantity of extract was dissolved in 2N hydrochloric acid and filtered. One mL of this solution washed with about 10 mL chloroform for three times in a separation funnel.…”

Antioxidant Potential Profile of Pajanelia Longifolia (Willd.) K. Schuman.; Potential New Sources of Natural Antioxidant.

“…5 ml of bromocresol green solution and 5 ml of phosphate buffer were added. The mixture was extracted with chloroform by shaking; the extracted mixture was then diluted with chloroform [9]. The absorbance was measured at spectrum of 470 nm, and the result was expressed as μg atropine equivalent/mg of plant extract.…”