Estimation of the level of tumor necrosis factor-α in gingival crevicular fluid and serum in periodontal health and disease: A biochemical study

Gokul, Kalpana; Faizuddin, Mohamed; Pradeep, A.R.

doi:10.4103/0970-9290.102221

Cited by 33 publications

(42 citation statements)

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“…In periodontal disease, TNF-α levels rise, and TNF-α is held responsible for bone destruction, especially in inflammatory conditions [35]. In some studies, high TNF-α levels were reported in gingivitis as well as in periodontitis [36,37]. Similarly, in our study, TNF-α levels were found to be higher in gingivitis and periodontitis compared to those of the healthy control group, while there was no difference between gingivitis and periodontitis.…”

Section: Discussioncontrasting

confidence: 53%

Evaluation of the salivary levels of visfatin, chemerin, and progranulin in periodontal inflammation

Özcan¹,

Saygun²,

Serdar

et al. 2014

Clin Oral Invest

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Section: Discussioncontrasting

confidence: 53%

Evaluation of the salivary levels of visfatin, chemerin, and progranulin in periodontal inflammation

Özcan¹,

Saygun²,

Serdar

et al. 2014

Clin Oral Invest

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“…In other studies there was substantial elevation in TNF- α concentration from healthy to diseased sites [14, 70, 91]. Thus, TNF- α concentrations may also be used as a predictor of disease progression.…”

Section: Discussionmentioning

confidence: 90%

“…Reis et al [91] showed that TNF- α levels were significantly higher in diseased sites compared to nondiseased sites. Gokul [14] showed a highly significant difference between healthy subjects and patients with gingivitis and CP. Kurtiş et al [70] showed highly significant difference between healthy subjects and patients with CP and GAgP.…”

Section: Resultsmentioning

confidence: 99%

Identification of Gingival Crevicular Fluid Sampling, Analytical Methods, and Oral Biomarkers for the Diagnosis and Monitoring of Periodontal Diseases: A Systematic Review

et al. 2016

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Background. Several studies in the last decades have focused on finding a precise method for the diagnosis of periodontal disease in its early stages. Aim. To evaluate from current scientific literature the most common and precise method for gingival crevicular fluid (GCF) sample collection, biomarker analytical methods, and the variability of biomarker quantification, even when using the same analytical technique. Methodology. An electronic search was conducted on in vivo studies that presented clinical data on techniques used for GCF collection and biomarker analysis. Results. The results showed that 71.1%, 24.7%, and 4.1% of the studies used absorption, microcapillary, and washing techniques, respectively, in their gingival crevicular fluid collection. 73.1% of the researchers analyzed their samples by using enzyme-linked immunosorbent assay (ELISA). 22.6%, 19.5%, and 18.5% of the researchers included interleukin-1 beta (IL-1β), matrix metalloproteinase-8 (MMP-8), and tumor necrosis factor-alpha (TNF-α), respectively, in their studies as biomarkers for periodontal disease. Conclusion. IL-1β can be considered among the most common biomarkers that give precise results and can be used as an indicator of periodontal disease progression. Furthermore, paper strips are the most convenient and accurate method for gingival crevicular fluid collection, while enzyme-linked immunosorbent assay can be considered the most conventional method for the diagnosis of biofluids.

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“…In comparison to our study, Reis et al considered only PPD to define chronic periodontal disease while it was unclear what criteria was used for non‐diseased sites. Gokul et al compared gingivitis, chronic periodontitis, and healthy individuals showing significantly elevated levels of TNF‐α in gingivitis and chronic periodontitis groups when compared to healthy subjects, while no significant differences were detected between gingivitis and periodontitis groups …”

Section: Discussionmentioning

confidence: 99%

Sclerostin and WNT‐5a gingival protein levels in chronic periodontitis and health

Chatzopoulos

Mansky

Lunos

et al. 2019

J of Periodontal Research

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Background and Objective Wnt signaling pathways regulate osteoblast differentiation and bone formation and are associated with inflammatory responses driven by innate and adaptive immunity via the NF‐κB pathway. The aim of this study was to compare the levels of sclerostin (SOST), WNT‐5a, and TNF‐α between chronic periodontitis and periodontally healthy sites and determine their value as diagnostic markers of chronic periodontitis. Material and Methods In a cross‐sectional assessment 25 chronic periodontitis cases and 25 periodontally healthy controls were selected upon clinical and radiographic periodontal evaluation. Gingival crevicular fluid (GCF) was collected cross‐sectionally from diseased and healthy sites in periodontitis patients and from healthy sites in each control subject. In a subgroup analysis, ten patients with generalized moderate and severe chronic periodontitis and ten generalized periodontally healthy individuals were included. The protein levels of SOST, WNT‐5a, and TNF‐α in GCF were measured by sandwich ELISA. The Shapiro‐Wilk test was utilized to assess the normality of the distribution and non‐parametric comparisons were performed. Results The protein levels of SOST were significantly higher in the generalized moderate and severe chronic periodontitis subgroup when compared to the generalized healthy (P = 0.002), while the WNT‐5a and TNF‐α GCF total amounts were similar (P > 0.05). Diseased sites in the periodontitis patients exhibited significantly higher total protein levels of WNT‐5a than in healthy sites (P = 0.017), whereas no differences were detected for SOST and TNF‐α (P > 0.05). The total protein levels of SOST, WNT‐5a, and TNF‐α in GCF were similar in periodontitis and non‐periodontitis patients (P > 0.05). Conclusions Sclerostin and WNT‐5a gingival protein levels demonstrated a high diagnostic value for generalized moderate and severe chronic periodontitis, while a low accuracy was detected for localized chronic periodontitis.

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Estimation of the level of tumor necrosis factor-α in gingival crevicular fluid and serum in periodontal health and disease: A biochemical study

Cited by 33 publications

References 0 publications

Evaluation of the salivary levels of visfatin, chemerin, and progranulin in periodontal inflammation

Evaluation of the salivary levels of visfatin, chemerin, and progranulin in periodontal inflammation

Identification of Gingival Crevicular Fluid Sampling, Analytical Methods, and Oral Biomarkers for the Diagnosis and Monitoring of Periodontal Diseases: A Systematic Review

Sclerostin and WNT‐5a gingival protein levels in chronic periodontitis and health

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